Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS10965

Experiment: R2A with Nickel (II) chloride 0.35 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS10955 and RR42_RS10960 are separated by 41 nucleotidesRR42_RS10960 and RR42_RS10965 are separated by 11 nucleotidesRR42_RS10965 and RR42_RS10970 overlap by 4 nucleotidesRR42_RS10970 and RR42_RS10975 are separated by 172 nucleotides RR42_RS10955: RR42_RS10955 - GntR family transcriptional regulator, at 2,340,305 to 2,341,057 _RS10955 RR42_RS10960: RR42_RS10960 - 3-hydroxyisobutyrate dehydrogenase, at 2,341,099 to 2,341,995 _RS10960 RR42_RS10965: RR42_RS10965 - membrane protein, at 2,342,007 to 2,343,311 _RS10965 RR42_RS10970: RR42_RS10970 - aldolase, at 2,343,308 to 2,343,946 _RS10970 RR42_RS10975: RR42_RS10975 - MFS transporter, at 2,344,119 to 2,345,450 _RS10975 Position (kb) 2342 2343 2344Strain fitness (log2 ratio) -1 0 1 2at 2341.181 kb on + strandat 2341.181 kb on + strandat 2341.181 kb on + strandat 2341.181 kb on + strandat 2341.181 kb on + strandat 2341.182 kb on - strandat 2341.182 kb on - strandat 2341.532 kb on - strand, within RR42_RS10960at 2341.532 kb on - strand, within RR42_RS10960at 2341.532 kb on - strand, within RR42_RS10960at 2341.576 kb on + strand, within RR42_RS10960at 2341.648 kb on - strand, within RR42_RS10960at 2341.711 kb on + strand, within RR42_RS10960at 2341.711 kb on + strand, within RR42_RS10960at 2341.712 kb on - strand, within RR42_RS10960at 2341.770 kb on + strand, within RR42_RS10960at 2341.771 kb on - strand, within RR42_RS10960at 2341.786 kb on + strand, within RR42_RS10960at 2341.786 kb on + strand, within RR42_RS10960at 2341.809 kb on + strand, within RR42_RS10960at 2341.955 kb on + strandat 2341.955 kb on + strandat 2341.956 kb on - strandat 2341.956 kb on - strandat 2341.956 kb on - strandat 2341.956 kb on - strandat 2342.290 kb on - strand, within RR42_RS10965at 2342.325 kb on - strand, within RR42_RS10965at 2342.644 kb on + strand, within RR42_RS10965at 2342.663 kb on + strand, within RR42_RS10965at 2342.663 kb on + strand, within RR42_RS10965at 2342.663 kb on + strand, within RR42_RS10965at 2342.663 kb on + strand, within RR42_RS10965at 2342.664 kb on - strand, within RR42_RS10965at 2342.977 kb on - strand, within RR42_RS10965at 2343.362 kb on + strandat 2343.362 kb on + strandat 2343.363 kb on - strandat 2343.363 kb on - strandat 2343.397 kb on + strand, within RR42_RS10970at 2343.398 kb on - strand, within RR42_RS10970at 2343.551 kb on + strand, within RR42_RS10970at 2343.586 kb on + strand, within RR42_RS10970at 2343.587 kb on - strand, within RR42_RS10970at 2343.587 kb on - strand, within RR42_RS10970at 2343.668 kb on + strand, within RR42_RS10970at 2343.668 kb on + strand, within RR42_RS10970at 2343.668 kb on + strand, within RR42_RS10970at 2343.669 kb on - strand, within RR42_RS10970at 2343.669 kb on - strand, within RR42_RS10970at 2343.669 kb on - strand, within RR42_RS10970at 2343.669 kb on - strand, within RR42_RS10970at 2343.669 kb on - strand, within RR42_RS10970at 2343.688 kb on + strand, within RR42_RS10970at 2343.689 kb on - strand, within RR42_RS10970at 2343.963 kb on + strandat 2343.964 kb on - strandat 2343.964 kb on - strandat 2343.976 kb on + strandat 2344.072 kb on + strandat 2344.107 kb on - strandat 2344.107 kb on - strandat 2344.107 kb on - strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.124 kb on + strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.125 kb on - strandat 2344.236 kb on + strandat 2344.236 kb on + strandat 2344.237 kb on - strandat 2344.237 kb on - strandat 2344.237 kb on - strandat 2344.237 kb on - strandat 2344.248 kb on + strandat 2344.249 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM
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2,341,181 + -0.0
2,341,181 + +0.0
2,341,181 + +1.7
2,341,181 + -0.0
2,341,181 + +0.2
2,341,182 - -0.9
2,341,182 - -1.0
2,341,532 - RR42_RS10960 0.48 +1.6
2,341,532 - RR42_RS10960 0.48 +0.6
2,341,532 - RR42_RS10960 0.48 +0.6
2,341,576 + RR42_RS10960 0.53 +1.2
2,341,648 - RR42_RS10960 0.61 +0.2
2,341,711 + RR42_RS10960 0.68 +0.0
2,341,711 + RR42_RS10960 0.68 -0.0
2,341,712 - RR42_RS10960 0.68 -0.8
2,341,770 + RR42_RS10960 0.75 +0.3
2,341,771 - RR42_RS10960 0.75 -0.0
2,341,786 + RR42_RS10960 0.77 +0.4
2,341,786 + RR42_RS10960 0.77 +0.1
2,341,809 + RR42_RS10960 0.79 +0.2
2,341,955 + -1.4
2,341,955 + +0.2
2,341,956 - -0.5
2,341,956 - +0.1
2,341,956 - -0.2
2,341,956 - +0.2
2,342,290 - RR42_RS10965 0.22 +0.5
2,342,325 - RR42_RS10965 0.24 +0.5
2,342,644 + RR42_RS10965 0.49 -0.4
2,342,663 + RR42_RS10965 0.50 +0.4
2,342,663 + RR42_RS10965 0.50 +0.7
2,342,663 + RR42_RS10965 0.50 -0.8
2,342,663 + RR42_RS10965 0.50 +0.1
2,342,664 - RR42_RS10965 0.50 +0.1
2,342,977 - RR42_RS10965 0.74 -0.6
2,343,362 + -0.5
2,343,362 + +0.0
2,343,363 - +0.8
2,343,363 - +1.4
2,343,397 + RR42_RS10970 0.14 +2.0
2,343,398 - RR42_RS10970 0.14 -1.7
2,343,551 + RR42_RS10970 0.38 +0.1
2,343,586 + RR42_RS10970 0.44 +0.2
2,343,587 - RR42_RS10970 0.44 +1.3
2,343,587 - RR42_RS10970 0.44 +1.3
2,343,668 + RR42_RS10970 0.56 +0.1
2,343,668 + RR42_RS10970 0.56 -0.6
2,343,668 + RR42_RS10970 0.56 +0.5
2,343,669 - RR42_RS10970 0.56 -1.0
2,343,669 - RR42_RS10970 0.56 +0.3
2,343,669 - RR42_RS10970 0.56 +0.2
2,343,669 - RR42_RS10970 0.56 +1.1
2,343,669 - RR42_RS10970 0.56 +0.6
2,343,688 + RR42_RS10970 0.59 +0.6
2,343,689 - RR42_RS10970 0.60 -0.3
2,343,963 + -1.2
2,343,964 - -0.4
2,343,964 - +0.5
2,343,976 + +0.1
2,344,072 + -0.1
2,344,107 - -1.1
2,344,107 - +1.4
2,344,107 - +0.2
2,344,124 + +0.6
2,344,124 + +0.9
2,344,124 + +0.3
2,344,124 + +1.0
2,344,124 + +0.4
2,344,124 + +0.3
2,344,124 + +0.4
2,344,124 + +0.0
2,344,124 + -0.2
2,344,124 + +0.5
2,344,124 + +0.1
2,344,124 + +0.2
2,344,124 + -0.2
2,344,124 + +0.2
2,344,124 + +0.2
2,344,125 - -0.5
2,344,125 - +0.1
2,344,125 - +0.6
2,344,125 - +0.1
2,344,125 - +0.4
2,344,125 - -0.7
2,344,125 - +0.1
2,344,125 - +0.7
2,344,125 - +0.2
2,344,125 - +0.5
2,344,236 + -1.5
2,344,236 + +0.3
2,344,237 - +0.3
2,344,237 - +0.0
2,344,237 - -0.3
2,344,237 - +0.4
2,344,248 + +0.7
2,344,249 - +0.1

Or see this region's nucleotide sequence