Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06565 and RR42_RS06570 are separated by 14 nucleotides RR42_RS06570 and RR42_RS06575 are separated by 202 nucleotides RR42_RS06575 and RR42_RS06580 are separated by 62 nucleotides RR42_RS06580 and RR42_RS06585 are separated by 15 nucleotides
RR42_RS06565: RR42_RS06565 - metal-binding protein, at 1,435,290 to 1,435,784
_RS06565
RR42_RS06570: RR42_RS06570 - 3-hydroxybutyrate dehydrogenase, at 1,435,799 to 1,436,575
_RS06570
RR42_RS06575: RR42_RS06575 - alpha/beta hydrolase, at 1,436,778 to 1,437,665
_RS06575
RR42_RS06580: RR42_RS06580 - aminoacrylate peracid reductase, at 1,437,728 to 1,438,090
_RS06580
RR42_RS06585: RR42_RS06585 - GTP pyrophosphokinase, at 1,438,106 to 1,440,328
_RS06585
Position (kb)
1436
1437
1438 Strain fitness (log2 ratio)
-2
-1
0
1 at 1435.967 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.570 kb on - strand at 1436.686 kb on + strand at 1436.687 kb on - strand at 1436.687 kb on - strand at 1436.701 kb on - strand at 1436.760 kb on + strand at 1436.760 kb on + strand at 1436.848 kb on - strand at 1436.848 kb on - strand at 1436.956 kb on - strand, within RR42_RS06575 at 1436.956 kb on - strand, within RR42_RS06575 at 1436.978 kb on - strand, within RR42_RS06575 at 1436.978 kb on - strand, within RR42_RS06575 at 1437.022 kb on - strand, within RR42_RS06575 at 1437.036 kb on + strand at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.388 kb on - strand, within RR42_RS06575 at 1437.499 kb on + strand, within RR42_RS06575 at 1437.639 kb on - strand at 1437.717 kb on - strand at 1437.780 kb on - strand, within RR42_RS06580 at 1437.803 kb on + strand, within RR42_RS06580 at 1437.803 kb on + strand, within RR42_RS06580 at 1437.804 kb on - strand, within RR42_RS06580 at 1437.804 kb on - strand, within RR42_RS06580 at 1438.006 kb on + strand, within RR42_RS06580 at 1438.053 kb on - strand, within RR42_RS06580 at 1438.411 kb on + strand, within RR42_RS06585 at 1438.411 kb on + strand, within RR42_RS06585 at 1438.637 kb on + strand, within RR42_RS06585 at 1438.637 kb on + strand, within RR42_RS06585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 1,435,967 - RR42_RS06570 0.22 +0.1 1,436,119 - RR42_RS06570 0.41 -0.4 1,436,119 - RR42_RS06570 0.41 +1.1 1,436,119 - RR42_RS06570 0.41 -0.2 1,436,119 - RR42_RS06570 0.41 -2.4 1,436,570 - +0.1 1,436,686 + +1.3 1,436,687 - -0.1 1,436,687 - -0.8 1,436,701 - +0.7 1,436,760 + +0.1 1,436,760 + +0.4 1,436,848 - -0.5 1,436,848 - -0.3 1,436,956 - RR42_RS06575 0.20 -0.4 1,436,956 - RR42_RS06575 0.20 -0.3 1,436,978 - RR42_RS06575 0.23 +0.1 1,436,978 - RR42_RS06575 0.23 -0.9 1,437,022 - RR42_RS06575 0.27 -0.4 1,437,036 + +0.0 1,437,037 - RR42_RS06575 0.29 +0.2 1,437,037 - RR42_RS06575 0.29 +0.0 1,437,037 - RR42_RS06575 0.29 +0.2 1,437,037 - RR42_RS06575 0.29 -1.7 1,437,037 - RR42_RS06575 0.29 -0.3 1,437,222 + RR42_RS06575 0.50 +1.4 1,437,222 + RR42_RS06575 0.50 +0.5 1,437,223 - RR42_RS06575 0.50 -1.5 1,437,223 - RR42_RS06575 0.50 -0.8 1,437,388 - RR42_RS06575 0.69 +1.3 1,437,499 + RR42_RS06575 0.81 -0.2 1,437,639 - -0.2 1,437,717 - -1.1 1,437,780 - RR42_RS06580 0.14 +0.4 1,437,803 + RR42_RS06580 0.21 -0.7 1,437,803 + RR42_RS06580 0.21 -0.6 1,437,804 - RR42_RS06580 0.21 -1.4 1,437,804 - RR42_RS06580 0.21 +0.0 1,438,006 + RR42_RS06580 0.77 -0.5 1,438,053 - RR42_RS06580 0.90 -1.6 1,438,411 + RR42_RS06585 0.14 +0.8 1,438,411 + RR42_RS06585 0.14 -0.5 1,438,637 + RR42_RS06585 0.24 -0.3 1,438,637 + RR42_RS06585 0.24 -0.7
Or see this region's nucleotide sequence