Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05890

Experiment: R2A with Nickel (II) chloride 0.35 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05885 and RR42_RS05890 are separated by 258 nucleotidesRR42_RS05890 and RR42_RS05895 overlap by 4 nucleotidesRR42_RS05895 and RR42_RS05900 are separated by 71 nucleotides RR42_RS05885: RR42_RS05885 - hypothetical protein, at 1,291,737 to 1,295,921 _RS05885 RR42_RS05890: RR42_RS05890 - glutamine-synthetase adenylyltransferase, at 1,296,180 to 1,299,140 _RS05890 RR42_RS05895: RR42_RS05895 - rhombosortase, at 1,299,137 to 1,299,772 _RS05895 RR42_RS05900: RR42_RS05900 - peptidase, at 1,299,844 to 1,301,529 _RS05900 Position (kb) 1296 1297 1298 1299 1300Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1295.248 kb on + strand, within RR42_RS05885at 1295.248 kb on + strand, within RR42_RS05885at 1295.248 kb on + strand, within RR42_RS05885at 1295.249 kb on - strand, within RR42_RS05885at 1295.249 kb on - strand, within RR42_RS05885at 1295.250 kb on - strand, within RR42_RS05885at 1295.272 kb on - strand, within RR42_RS05885at 1295.713 kb on + strandat 1295.713 kb on + strandat 1295.713 kb on + strandat 1295.714 kb on - strandat 1295.714 kb on - strandat 1295.714 kb on - strandat 1295.714 kb on - strandat 1295.717 kb on + strandat 1295.718 kb on - strandat 1296.090 kb on + strandat 1296.090 kb on + strandat 1296.091 kb on - strandat 1296.091 kb on - strandat 1296.091 kb on - strandat 1296.091 kb on - strandat 1296.094 kb on - strandat 1296.094 kb on - strandat 1296.173 kb on - strandat 1296.173 kb on - strandat 1296.372 kb on + strandat 1296.372 kb on + strandat 1296.373 kb on - strandat 1296.385 kb on - strandat 1296.385 kb on - strandat 1296.385 kb on - strandat 1296.393 kb on + strandat 1296.393 kb on + strandat 1296.394 kb on - strandat 1296.660 kb on + strand, within RR42_RS05890at 1296.699 kb on + strand, within RR42_RS05890at 1296.700 kb on - strand, within RR42_RS05890at 1296.816 kb on + strand, within RR42_RS05890at 1296.816 kb on + strand, within RR42_RS05890at 1296.817 kb on - strand, within RR42_RS05890at 1296.871 kb on - strand, within RR42_RS05890at 1296.871 kb on - strand, within RR42_RS05890at 1296.871 kb on - strand, within RR42_RS05890at 1296.930 kb on + strand, within RR42_RS05890at 1296.930 kb on + strand, within RR42_RS05890at 1296.931 kb on - strand, within RR42_RS05890at 1296.931 kb on - strand, within RR42_RS05890at 1297.011 kb on + strand, within RR42_RS05890at 1297.011 kb on + strand, within RR42_RS05890at 1297.044 kb on + strand, within RR42_RS05890at 1297.044 kb on + strand, within RR42_RS05890at 1297.045 kb on - strand, within RR42_RS05890at 1297.488 kb on + strand, within RR42_RS05890at 1297.488 kb on + strand, within RR42_RS05890at 1297.488 kb on + strand, within RR42_RS05890at 1298.025 kb on + strand, within RR42_RS05890at 1298.025 kb on + strand, within RR42_RS05890at 1298.025 kb on + strand, within RR42_RS05890at 1298.419 kb on - strand, within RR42_RS05890at 1298.451 kb on + strand, within RR42_RS05890at 1298.461 kb on - strand, within RR42_RS05890at 1298.859 kb on + strandat 1298.859 kb on - strandat 1298.860 kb on - strandat 1298.860 kb on - strandat 1298.860 kb on - strandat 1298.860 kb on - strandat 1298.860 kb on - strandat 1299.195 kb on + strandat 1299.229 kb on + strand, within RR42_RS05895at 1299.229 kb on + strand, within RR42_RS05895at 1299.263 kb on + strand, within RR42_RS05895at 1299.721 kb on - strandat 1299.771 kb on - strandat 1300.074 kb on - strand, within RR42_RS05900at 1300.074 kb on - strand, within RR42_RS05900at 1300.074 kb on - strand, within RR42_RS05900at 1300.074 kb on - strand, within RR42_RS05900

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM
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1,295,248 + RR42_RS05885 0.84 -0.7
1,295,248 + RR42_RS05885 0.84 -2.3
1,295,248 + RR42_RS05885 0.84 -1.3
1,295,249 - RR42_RS05885 0.84 -1.9
1,295,249 - RR42_RS05885 0.84 -0.7
1,295,250 - RR42_RS05885 0.84 -1.0
1,295,272 - RR42_RS05885 0.84 -2.0
1,295,713 + -1.3
1,295,713 + -1.6
1,295,713 + +1.3
1,295,714 - -0.5
1,295,714 - -1.8
1,295,714 - -0.3
1,295,714 - +0.2
1,295,717 + +0.3
1,295,718 - -2.0
1,296,090 + +0.9
1,296,090 + -0.6
1,296,091 - +0.0
1,296,091 - -0.4
1,296,091 - -1.0
1,296,091 - +3.0
1,296,094 - -0.3
1,296,094 - -0.6
1,296,173 - +0.2
1,296,173 - +0.2
1,296,372 + -0.2
1,296,372 + +0.4
1,296,373 - -0.8
1,296,385 - +0.3
1,296,385 - +0.8
1,296,385 - -1.4
1,296,393 + -0.3
1,296,393 + +0.1
1,296,394 - -0.8
1,296,660 + RR42_RS05890 0.16 -0.7
1,296,699 + RR42_RS05890 0.18 -2.2
1,296,700 - RR42_RS05890 0.18 -1.6
1,296,816 + RR42_RS05890 0.21 -0.3
1,296,816 + RR42_RS05890 0.21 -2.7
1,296,817 - RR42_RS05890 0.22 -1.1
1,296,871 - RR42_RS05890 0.23 -0.9
1,296,871 - RR42_RS05890 0.23 -0.7
1,296,871 - RR42_RS05890 0.23 -0.5
1,296,930 + RR42_RS05890 0.25 -1.4
1,296,930 + RR42_RS05890 0.25 -1.3
1,296,931 - RR42_RS05890 0.25 -1.6
1,296,931 - RR42_RS05890 0.25 -1.0
1,297,011 + RR42_RS05890 0.28 -0.7
1,297,011 + RR42_RS05890 0.28 +1.3
1,297,044 + RR42_RS05890 0.29 -0.3
1,297,044 + RR42_RS05890 0.29 +0.7
1,297,045 - RR42_RS05890 0.29 -0.7
1,297,488 + RR42_RS05890 0.44 -0.2
1,297,488 + RR42_RS05890 0.44 +0.5
1,297,488 + RR42_RS05890 0.44 -1.9
1,298,025 + RR42_RS05890 0.62 -0.2
1,298,025 + RR42_RS05890 0.62 -0.5
1,298,025 + RR42_RS05890 0.62 +0.3
1,298,419 - RR42_RS05890 0.76 -0.6
1,298,451 + RR42_RS05890 0.77 +0.2
1,298,461 - RR42_RS05890 0.77 -0.2
1,298,859 + -0.9
1,298,859 - -0.0
1,298,860 - -1.5
1,298,860 - -0.5
1,298,860 - -0.6
1,298,860 - -1.2
1,298,860 - +0.9
1,299,195 + +0.3
1,299,229 + RR42_RS05895 0.14 +0.7
1,299,229 + RR42_RS05895 0.14 -0.8
1,299,263 + RR42_RS05895 0.20 +0.9
1,299,721 - -0.1
1,299,771 - -0.7
1,300,074 - RR42_RS05900 0.14 +1.3
1,300,074 - RR42_RS05900 0.14 -0.2
1,300,074 - RR42_RS05900 0.14 +0.8
1,300,074 - RR42_RS05900 0.14 -0.3

Or see this region's nucleotide sequence