Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02800 and RR42_RS02805 are separated by 116 nucleotides RR42_RS02805 and RR42_RS02810 overlap by 4 nucleotides RR42_RS02810 and RR42_RS02815 are separated by 17 nucleotides RR42_RS02815 and RR42_RS02820 are separated by 29 nucleotides
RR42_RS02800: RR42_RS02800 - glyoxalase I, at 624,172 to 624,579
_RS02800
RR42_RS02805: RR42_RS02805 - metal-dependent hydrolase, at 624,696 to 625,580
_RS02805
RR42_RS02810: RR42_RS02810 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 625,577 to 626,329
_RS02810
RR42_RS02815: RR42_RS02815 - D,D-heptose 1,7-bisphosphate phosphatase, at 626,347 to 626,925
_RS02815
RR42_RS02820: RR42_RS02820 - glycyl-tRNA synthetase subunit beta, at 626,955 to 629,087
_RS02820
Position (kb) 625
626
627 Strain fitness (log2 ratio) -2
-1
0
1 at 624.597 kb on - strand at 624.619 kb on - strand at 624.641 kb on + strand at 624.641 kb on + strand at 624.642 kb on - strand at 624.698 kb on - strand at 624.951 kb on - strand, within RR42_RS02805 at 624.976 kb on + strand, within RR42_RS02805 at 624.976 kb on + strand, within RR42_RS02805 at 624.977 kb on - strand, within RR42_RS02805 at 624.980 kb on + strand, within RR42_RS02805 at 624.981 kb on - strand, within RR42_RS02805 at 624.981 kb on - strand, within RR42_RS02805 at 625.375 kb on + strand, within RR42_RS02805 at 625.781 kb on + strand, within RR42_RS02810 at 625.782 kb on - strand, within RR42_RS02810 at 625.828 kb on - strand, within RR42_RS02810 at 626.061 kb on + strand, within RR42_RS02810 at 626.956 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 624,597 - +1.0 624,619 - -1.6 624,641 + +0.1 624,641 + +1.3 624,642 - +0.1 624,698 - +0.5 624,951 - RR42_RS02805 0.29 +0.4 624,976 + RR42_RS02805 0.32 +1.2 624,976 + RR42_RS02805 0.32 -1.1 624,977 - RR42_RS02805 0.32 -1.0 624,980 + RR42_RS02805 0.32 +0.2 624,981 - RR42_RS02805 0.32 +0.2 624,981 - RR42_RS02805 0.32 +1.3 625,375 + RR42_RS02805 0.77 -0.4 625,781 + RR42_RS02810 0.27 -2.7 625,782 - RR42_RS02810 0.27 -2.5 625,828 - RR42_RS02810 0.33 -0.8 626,061 + RR42_RS02810 0.64 -1.3 626,956 + -2.2
Or see this region's nucleotide sequence