Experiment: Glycerol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4 and Pf1N1B4_5 are separated by 183 nucleotides Pf1N1B4_5 and Pf1N1B4_6 are separated by 41 nucleotides Pf1N1B4_6 and Pf1N1B4_7 are separated by 63 nucleotides
Pf1N1B4_4 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 3,678 to 4,598
_4
Pf1N1B4_5 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 4,782 to 4,922
_5
Pf1N1B4_6 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 4,964 to 5,401
_6
Pf1N1B4_7 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 5,465 to 6,319
_7
Position (kb) 4
5
6 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4.673 kb on - strand at 4.673 kb on - strand at 4.673 kb on - strand at 4.673 kb on - strand at 4.673 kb on - strand at 4.673 kb on - strand at 4.724 kb on - strand at 4.724 kb on - strand at 4.724 kb on - strand at 4.736 kb on - strand at 4.736 kb on - strand at 4.852 kb on - strand, within Pf1N1B4_5 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.103 kb on + strand, within Pf1N1B4_6 at 5.104 kb on - strand at 5.104 kb on - strand, within Pf1N1B4_6 at 5.104 kb on - strand, within Pf1N1B4_6 at 5.104 kb on - strand, within Pf1N1B4_6 at 5.104 kb on - strand, within Pf1N1B4_6 at 5.304 kb on + strand, within Pf1N1B4_6 at 5.305 kb on - strand, within Pf1N1B4_6 at 5.361 kb on - strand at 5.361 kb on - strand at 5.361 kb on - strand at 5.512 kb on - strand at 5.512 kb on - strand at 5.552 kb on + strand, within Pf1N1B4_7 at 5.560 kb on - strand, within Pf1N1B4_7 at 5.603 kb on - strand, within Pf1N1B4_7 at 5.753 kb on + strand at 5.753 kb on + strand, within Pf1N1B4_7 at 5.754 kb on - strand, within Pf1N1B4_7 at 5.754 kb on - strand, within Pf1N1B4_7 at 5.754 kb on - strand, within Pf1N1B4_7 at 5.887 kb on + strand, within Pf1N1B4_7 at 5.888 kb on - strand, within Pf1N1B4_7 at 5.928 kb on + strand, within Pf1N1B4_7 at 5.929 kb on - strand, within Pf1N1B4_7 at 5.929 kb on - strand, within Pf1N1B4_7 at 6.247 kb on - strand at 6.305 kb on - strand at 6.305 kb on - strand at 6.308 kb on + strand at 6.308 kb on + strand at 6.308 kb on + strand at 6.309 kb on - strand at 6.309 kb on - strand at 6.309 kb on - strand at 6.309 kb on - strand at 6.310 kb on + strand at 6.311 kb on - strand at 6.357 kb on + strand at 6.357 kb on + strand at 6.357 kb on + strand at 6.358 kb on - strand at 6.358 kb on - strand at 6.358 kb on - strand at 6.358 kb on - strand at 6.359 kb on + strand at 6.359 kb on + strand at 6.359 kb on + strand at 6.359 kb on + strand at 6.359 kb on + strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol (C) remove 4,673 - +0.2 4,673 - +1.1 4,673 - +0.1 4,673 - +0.2 4,673 - -0.3 4,673 - +0.4 4,724 - +1.7 4,724 - +0.2 4,724 - -0.3 4,736 - +0.1 4,736 - +0.3 4,852 - Pf1N1B4_5 0.50 -3.6 5,014 - Pf1N1B4_6 0.11 -3.6 5,014 - Pf1N1B4_6 0.11 -4.7 5,014 - Pf1N1B4_6 0.11 -3.4 5,014 - Pf1N1B4_6 0.11 -3.7 5,014 - Pf1N1B4_6 0.11 -1.3 5,014 - Pf1N1B4_6 0.11 -1.6 5,014 - Pf1N1B4_6 0.11 -1.8 5,014 - Pf1N1B4_6 0.11 -2.8 5,103 + Pf1N1B4_6 0.32 -2.6 5,104 - -1.3 5,104 - Pf1N1B4_6 0.32 -1.3 5,104 - Pf1N1B4_6 0.32 -1.3 5,104 - Pf1N1B4_6 0.32 -2.3 5,104 - Pf1N1B4_6 0.32 -2.8 5,304 + Pf1N1B4_6 0.78 -3.6 5,305 - Pf1N1B4_6 0.78 -2.6 5,361 - -1.3 5,361 - -2.6 5,361 - -1.3 5,512 - -0.4 5,512 - +0.3 5,552 + Pf1N1B4_7 0.10 -0.8 5,560 - Pf1N1B4_7 0.11 -0.3 5,603 - Pf1N1B4_7 0.16 +1.2 5,753 + -0.3 5,753 + Pf1N1B4_7 0.34 +0.7 5,754 - Pf1N1B4_7 0.34 -1.1 5,754 - Pf1N1B4_7 0.34 +0.1 5,754 - Pf1N1B4_7 0.34 +1.1 5,887 + Pf1N1B4_7 0.49 -0.3 5,888 - Pf1N1B4_7 0.49 +0.1 5,928 + Pf1N1B4_7 0.54 -0.7 5,929 - Pf1N1B4_7 0.54 +0.5 5,929 - Pf1N1B4_7 0.54 +1.3 6,247 - +0.3 6,305 - -0.3 6,305 - +1.7 6,308 + -1.3 6,308 + -1.1 6,308 + +0.6 6,309 - -0.3 6,309 - +0.3 6,309 - -0.8 6,309 - +2.1 6,310 + +0.7 6,311 - -1.0 6,357 + -0.3 6,357 + +0.1 6,357 + +2.7 6,358 - -0.3 6,358 - +1.5 6,358 - +2.3 6,358 - -0.7 6,359 + -0.4 6,359 + +0.4 6,359 + +1.0 6,359 + -0.3 6,359 + +2.3 6,360 - +1.2 6,360 - -2.3 6,360 - -0.8 6,360 - -0.7 6,360 - +1.3 6,360 - +0.3 6,360 - +0.5
Or see this region's nucleotide sequence