Experiment: N2 fixation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04435 and MMJJ_RS04440 are separated by 5 nucleotides MMJJ_RS04440 and MMJJ_RS04445 are separated by 334 nucleotides MMJJ_RS04445 and MMJJ_RS04450 are separated by 84 nucleotides MMJJ_RS04450 and MMJJ_RS04455 are separated by 89 nucleotides
MMJJ_RS04435: MMJJ_RS04435 - phosphotransferase, at 827,051 to 827,977
_RS04435
MMJJ_RS04440: MMJJ_RS04440 - hypothetical protein, at 827,983 to 828,360
_RS04440
MMJJ_RS04445: MMJJ_RS04445 - tRNA-Leu, at 828,695 to 828,779
_RS04445
MMJJ_RS04450: MMJJ_RS04450 - diphthamide biosynthesis enzyme Dph2, at 828,864 to 829,793
_RS04450
MMJJ_RS04455: MMJJ_RS04455 - phosphomethylpyrimidine synthase, at 829,883 to 831,163
_RS04455
Position (kb) 828
829
830 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 827.887 kb on - strand at 827.900 kb on - strand at 827.900 kb on - strand at 827.928 kb on + strand at 827.929 kb on - strand at 827.929 kb on - strand at 827.929 kb on - strand at 827.968 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.981 kb on + strand at 827.981 kb on + strand at 827.985 kb on + strand at 827.985 kb on + strand at 827.986 kb on - strand at 827.987 kb on + strand at 827.992 kb on + strand at 828.010 kb on + strand at 828.222 kb on - strand, within MMJJ_RS04440 at 828.223 kb on + strand, within MMJJ_RS04440 at 828.223 kb on + strand, within MMJJ_RS04440 at 828.225 kb on - strand, within MMJJ_RS04440 at 828.225 kb on - strand, within MMJJ_RS04440 at 828.226 kb on + strand, within MMJJ_RS04440 at 828.226 kb on + strand, within MMJJ_RS04440 at 828.226 kb on + strand, within MMJJ_RS04440 at 828.229 kb on - strand, within MMJJ_RS04440 at 828.230 kb on + strand, within MMJJ_RS04440 at 828.248 kb on - strand, within MMJJ_RS04440 at 828.289 kb on - strand, within MMJJ_RS04440 at 828.306 kb on + strand, within MMJJ_RS04440 at 828.349 kb on - strand at 828.350 kb on + strand at 828.376 kb on - strand at 828.389 kb on - strand at 828.393 kb on + strand at 828.487 kb on + strand at 828.496 kb on + strand at 828.535 kb on - strand at 828.866 kb on + strand at 828.868 kb on - strand at 828.869 kb on + strand at 828.922 kb on - strand at 829.004 kb on - strand, within MMJJ_RS04450 at 829.009 kb on - strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.030 kb on + strand, within MMJJ_RS04450 at 829.044 kb on - strand, within MMJJ_RS04450 at 829.044 kb on - strand, within MMJJ_RS04450 at 829.045 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.070 kb on + strand, within MMJJ_RS04450 at 829.079 kb on - strand, within MMJJ_RS04450 at 829.090 kb on + strand, within MMJJ_RS04450 at 829.180 kb on + strand, within MMJJ_RS04450 at 829.182 kb on - strand, within MMJJ_RS04450 at 829.183 kb on + strand, within MMJJ_RS04450 at 829.259 kb on - strand, within MMJJ_RS04450 at 829.260 kb on + strand, within MMJJ_RS04450 at 829.290 kb on - strand, within MMJJ_RS04450 at 829.296 kb on + strand, within MMJJ_RS04450 at 829.297 kb on - strand, within MMJJ_RS04450 at 829.297 kb on - strand, within MMJJ_RS04450 at 829.298 kb on + strand, within MMJJ_RS04450 at 829.307 kb on - strand, within MMJJ_RS04450 at 829.307 kb on - strand at 829.308 kb on + strand, within MMJJ_RS04450 at 829.308 kb on + strand, within MMJJ_RS04450 at 829.384 kb on + strand, within MMJJ_RS04450 at 829.572 kb on + strand, within MMJJ_RS04450 at 829.765 kb on + strand at 829.772 kb on - strand at 829.773 kb on + strand at 829.794 kb on + strand at 829.794 kb on + strand at 829.796 kb on - strand at 829.797 kb on + strand at 829.813 kb on + strand at 829.816 kb on - strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.832 kb on + strand at 829.832 kb on + strand at 829.834 kb on - strand at 829.834 kb on - strand at 829.835 kb on + strand at 829.838 kb on + strand at 829.849 kb on - strand at 829.869 kb on + strand at 829.871 kb on + strand at 830.174 kb on + strand at 830.249 kb on - strand, within MMJJ_RS04455
Per-strain Table
Position Strand Gene LocusTag Fraction N2 fixation, H2 media remove 827,887 - +0.6 827,900 - +0.8 827,900 - +0.5 827,928 + -0.2 827,929 - +0.0 827,929 - +0.6 827,929 - +0.4 827,968 - +0.5 827,980 - +0.3 827,980 - +0.2 827,980 - -0.5 827,980 - +0.2 827,980 - -1.3 827,980 - +1.0 827,980 - +2.4 827,981 + +0.2 827,981 + +0.3 827,985 + -0.4 827,985 + -1.1 827,986 - -1.7 827,987 + +0.9 827,992 + +0.5 828,010 + -0.1 828,222 - MMJJ_RS04440 0.63 -1.2 828,223 + MMJJ_RS04440 0.63 -1.4 828,223 + MMJJ_RS04440 0.63 -0.2 828,225 - MMJJ_RS04440 0.64 +1.5 828,225 - MMJJ_RS04440 0.64 +2.3 828,226 + MMJJ_RS04440 0.64 -0.0 828,226 + MMJJ_RS04440 0.64 +0.3 828,226 + MMJJ_RS04440 0.64 -1.3 828,229 - MMJJ_RS04440 0.65 -0.4 828,230 + MMJJ_RS04440 0.65 -0.2 828,248 - MMJJ_RS04440 0.70 +0.3 828,289 - MMJJ_RS04440 0.81 +1.2 828,306 + MMJJ_RS04440 0.85 -0.1 828,349 - -0.1 828,350 + -0.5 828,376 - +0.7 828,389 - +1.5 828,393 + +1.3 828,487 + -0.9 828,496 + +1.1 828,535 - +0.8 828,866 + -0.1 828,868 - -2.4 828,869 + -1.0 828,922 - -1.4 829,004 - MMJJ_RS04450 0.15 -1.0 829,009 - MMJJ_RS04450 0.16 +0.4 829,010 + MMJJ_RS04450 0.16 +0.4 829,010 + MMJJ_RS04450 0.16 +0.5 829,010 + MMJJ_RS04450 0.16 -2.7 829,030 + MMJJ_RS04450 0.18 -0.4 829,044 - MMJJ_RS04450 0.19 +0.7 829,044 - MMJJ_RS04450 0.19 +1.8 829,045 + MMJJ_RS04450 0.19 +1.2 829,068 + MMJJ_RS04450 0.22 -1.5 829,068 + MMJJ_RS04450 0.22 -0.7 829,068 + MMJJ_RS04450 0.22 -2.1 829,070 + MMJJ_RS04450 0.22 -1.1 829,079 - MMJJ_RS04450 0.23 -0.2 829,090 + MMJJ_RS04450 0.24 +0.8 829,180 + MMJJ_RS04450 0.34 -0.6 829,182 - MMJJ_RS04450 0.34 +6.8 829,183 + MMJJ_RS04450 0.34 -0.7 829,259 - MMJJ_RS04450 0.42 +0.7 829,260 + MMJJ_RS04450 0.43 +0.3 829,290 - MMJJ_RS04450 0.46 +2.3 829,296 + MMJJ_RS04450 0.46 +2.8 829,297 - MMJJ_RS04450 0.47 +3.7 829,297 - MMJJ_RS04450 0.47 -0.5 829,298 + MMJJ_RS04450 0.47 +0.5 829,307 - MMJJ_RS04450 0.48 -0.1 829,307 - +3.8 829,308 + MMJJ_RS04450 0.48 +0.8 829,308 + MMJJ_RS04450 0.48 +0.6 829,384 + MMJJ_RS04450 0.56 +0.9 829,572 + MMJJ_RS04450 0.76 -1.2 829,765 + +0.5 829,772 - +4.8 829,773 + -1.2 829,794 + -0.9 829,794 + +0.5 829,796 - +0.8 829,797 + -0.1 829,813 + -1.3 829,816 - -0.3 829,828 + -2.0 829,828 + -1.2 829,828 + +1.1 829,828 + -0.2 829,828 + -1.0 829,832 + +1.7 829,832 + -0.6 829,834 - +2.3 829,834 - -0.2 829,835 + +0.7 829,838 + +2.7 829,849 - +0.7 829,869 + +0.8 829,871 + +0.3 830,174 + +1.8 830,249 - MMJJ_RS04455 0.29 +2.3
Or see this region's nucleotide sequence