Experiment: N2 fixation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03510 and MMJJ_RS03515 are separated by 127 nucleotides MMJJ_RS03515 and MMJJ_RS03520 are separated by 89 nucleotides MMJJ_RS03520 and MMJJ_RS03525 are separated by 225 nucleotides
MMJJ_RS03510: MMJJ_RS03510 - acyl-CoA dehydratase activase, at 657,879 to 658,619
_RS03510
MMJJ_RS03515: MMJJ_RS03515 - GPR1/FUN34/YaaH family transporter, at 658,747 to 659,340
_RS03515
MMJJ_RS03520: MMJJ_RS03520 - hypothetical protein, at 659,430 to 659,744
_RS03520
MMJJ_RS03525: MMJJ_RS03525 - 2-hydroxyacyl-CoA dehydratase family protein, at 659,970 to 661,091
_RS03525
Position (kb) 659
660 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 658.480 kb on - strand, within MMJJ_RS03510 at 658.481 kb on + strand, within MMJJ_RS03510 at 658.481 kb on + strand, within MMJJ_RS03510 at 658.500 kb on + strand, within MMJJ_RS03510 at 658.523 kb on + strand, within MMJJ_RS03510 at 658.523 kb on + strand, within MMJJ_RS03510 at 658.553 kb on + strand at 658.595 kb on - strand at 658.596 kb on + strand at 658.635 kb on - strand at 658.643 kb on - strand at 658.646 kb on - strand at 658.648 kb on - strand at 658.649 kb on + strand at 658.674 kb on - strand at 658.675 kb on + strand at 658.675 kb on + strand at 658.692 kb on - strand at 658.693 kb on + strand at 658.693 kb on + strand at 658.698 kb on - strand at 658.698 kb on - strand at 658.698 kb on - strand at 658.698 kb on - strand at 658.698 kb on - strand at 658.699 kb on + strand at 658.699 kb on + strand at 658.699 kb on + strand at 658.699 kb on + strand at 658.700 kb on - strand at 658.700 kb on - strand at 658.700 kb on - strand at 658.701 kb on + strand at 658.701 kb on + strand at 658.701 kb on + strand at 658.701 kb on + strand at 658.701 kb on + strand at 658.701 kb on + strand at 658.701 kb on + strand at 658.701 kb on + strand at 658.703 kb on + strand at 658.712 kb on - strand at 658.740 kb on - strand at 658.741 kb on + strand at 658.741 kb on + strand at 658.760 kb on + strand at 658.770 kb on + strand at 658.866 kb on - strand, within MMJJ_RS03515 at 658.867 kb on + strand, within MMJJ_RS03515 at 658.893 kb on + strand, within MMJJ_RS03515 at 658.917 kb on + strand, within MMJJ_RS03515 at 658.928 kb on - strand, within MMJJ_RS03515 at 658.965 kb on + strand, within MMJJ_RS03515 at 658.971 kb on - strand, within MMJJ_RS03515 at 658.972 kb on + strand, within MMJJ_RS03515 at 658.978 kb on - strand, within MMJJ_RS03515 at 658.985 kb on - strand, within MMJJ_RS03515 at 659.087 kb on - strand, within MMJJ_RS03515 at 659.088 kb on + strand, within MMJJ_RS03515 at 659.144 kb on - strand, within MMJJ_RS03515 at 659.162 kb on - strand, within MMJJ_RS03515 at 659.162 kb on - strand, within MMJJ_RS03515 at 659.162 kb on - strand, within MMJJ_RS03515 at 659.165 kb on - strand, within MMJJ_RS03515 at 659.190 kb on - strand, within MMJJ_RS03515 at 659.190 kb on - strand, within MMJJ_RS03515 at 659.190 kb on - strand, within MMJJ_RS03515 at 659.190 kb on - strand, within MMJJ_RS03515 at 659.191 kb on + strand, within MMJJ_RS03515 at 659.210 kb on - strand, within MMJJ_RS03515 at 659.363 kb on + strand at 659.367 kb on - strand at 659.367 kb on - strand at 659.368 kb on + strand at 659.371 kb on - strand at 659.371 kb on - strand at 659.371 kb on - strand at 659.371 kb on - strand at 659.372 kb on + strand at 659.372 kb on + strand at 659.373 kb on - strand at 659.373 kb on - strand at 659.373 kb on - strand at 659.373 kb on - strand at 659.373 kb on - strand at 659.373 kb on - strand at 659.374 kb on + strand at 659.374 kb on + strand at 659.375 kb on - strand at 659.375 kb on - strand at 659.375 kb on - strand at 659.376 kb on + strand at 659.376 kb on + strand at 659.377 kb on - strand at 659.377 kb on - strand at 659.378 kb on + strand at 659.413 kb on - strand at 659.426 kb on + strand at 659.429 kb on - strand at 659.430 kb on + strand at 659.430 kb on + strand at 659.444 kb on + strand at 659.456 kb on + strand at 659.456 kb on + strand at 659.460 kb on + strand at 659.461 kb on - strand at 659.461 kb on - strand at 659.461 kb on - strand at 659.462 kb on + strand, within MMJJ_RS03520 at 659.462 kb on + strand, within MMJJ_RS03520 at 659.493 kb on + strand, within MMJJ_RS03520 at 659.493 kb on + strand, within MMJJ_RS03520 at 659.497 kb on - strand, within MMJJ_RS03520 at 659.498 kb on + strand, within MMJJ_RS03520 at 659.535 kb on + strand, within MMJJ_RS03520 at 659.554 kb on + strand, within MMJJ_RS03520 at 659.566 kb on - strand, within MMJJ_RS03520 at 659.566 kb on - strand, within MMJJ_RS03520 at 659.566 kb on - strand, within MMJJ_RS03520 at 659.566 kb on - strand, within MMJJ_RS03520 at 659.567 kb on + strand, within MMJJ_RS03520 at 659.567 kb on + strand, within MMJJ_RS03520 at 659.583 kb on + strand, within MMJJ_RS03520 at 659.585 kb on + strand, within MMJJ_RS03520 at 659.636 kb on - strand, within MMJJ_RS03520 at 659.636 kb on - strand, within MMJJ_RS03520 at 659.637 kb on + strand, within MMJJ_RS03520 at 659.642 kb on - strand, within MMJJ_RS03520 at 659.643 kb on + strand, within MMJJ_RS03520 at 659.643 kb on + strand, within MMJJ_RS03520 at 659.663 kb on + strand, within MMJJ_RS03520 at 659.698 kb on - strand, within MMJJ_RS03520 at 659.698 kb on - strand, within MMJJ_RS03520 at 659.698 kb on - strand, within MMJJ_RS03520 at 659.698 kb on - strand, within MMJJ_RS03520 at 659.698 kb on - strand, within MMJJ_RS03520 at 659.699 kb on + strand, within MMJJ_RS03520 at 659.699 kb on + strand, within MMJJ_RS03520 at 659.709 kb on - strand, within MMJJ_RS03520 at 659.709 kb on - strand, within MMJJ_RS03520 at 659.709 kb on - strand, within MMJJ_RS03520 at 659.709 kb on - strand, within MMJJ_RS03520 at 659.709 kb on - strand, within MMJJ_RS03520 at 659.710 kb on + strand, within MMJJ_RS03520 at 659.711 kb on - strand, within MMJJ_RS03520 at 659.711 kb on - strand, within MMJJ_RS03520 at 659.711 kb on - strand, within MMJJ_RS03520 at 659.712 kb on + strand, within MMJJ_RS03520 at 659.712 kb on + strand, within MMJJ_RS03520 at 659.712 kb on + strand, within MMJJ_RS03520 at 659.716 kb on - strand at 659.716 kb on - strand at 659.716 kb on - strand at 659.765 kb on + strand at 659.870 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.881 kb on - strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.882 kb on + strand at 659.889 kb on - strand at 659.889 kb on - strand at 659.889 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.891 kb on - strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.892 kb on + strand at 659.900 kb on - strand at 659.900 kb on - strand at 659.900 kb on - strand at 659.900 kb on - strand at 659.900 kb on - strand at 659.900 kb on - strand at 659.900 kb on - strand at 659.900 kb on - strand at 659.900 kb on - strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.901 kb on + strand at 659.981 kb on + strand at 660.098 kb on + strand, within MMJJ_RS03525 at 660.149 kb on - strand, within MMJJ_RS03525 at 660.175 kb on - strand, within MMJJ_RS03525 at 660.276 kb on + strand, within MMJJ_RS03525 at 660.276 kb on + strand, within MMJJ_RS03525 at 660.334 kb on - strand, within MMJJ_RS03525 at 660.334 kb on - strand, within MMJJ_RS03525 at 660.334 kb on - strand, within MMJJ_RS03525 at 660.335 kb on + strand, within MMJJ_RS03525 at 660.335 kb on + strand, within MMJJ_RS03525 at 660.343 kb on - strand, within MMJJ_RS03525 at 660.344 kb on + strand, within MMJJ_RS03525 at 660.410 kb on + strand, within MMJJ_RS03525 at 660.437 kb on - strand, within MMJJ_RS03525 at 660.481 kb on - strand, within MMJJ_RS03525 at 660.481 kb on - strand, within MMJJ_RS03525 at 660.482 kb on + strand, within MMJJ_RS03525 at 660.511 kb on - strand, within MMJJ_RS03525 at 660.512 kb on + strand, within MMJJ_RS03525 at 660.573 kb on - strand, within MMJJ_RS03525 at 660.573 kb on - strand, within MMJJ_RS03525 at 660.586 kb on - strand, within MMJJ_RS03525 at 660.586 kb on - strand, within MMJJ_RS03525 at 660.586 kb on - strand, within MMJJ_RS03525 at 660.586 kb on - strand, within MMJJ_RS03525 at 660.587 kb on + strand, within MMJJ_RS03525 at 660.641 kb on - strand, within MMJJ_RS03525 at 660.658 kb on + strand, within MMJJ_RS03525 at 660.734 kb on - strand, within MMJJ_RS03525
Per-strain Table
Position Strand Gene LocusTag Fraction N2 fixation, H2 media remove 658,480 - MMJJ_RS03510 0.81 -0.6 658,481 + MMJJ_RS03510 0.81 -0.9 658,481 + MMJJ_RS03510 0.81 -1.6 658,500 + MMJJ_RS03510 0.84 +0.6 658,523 + MMJJ_RS03510 0.87 +0.4 658,523 + MMJJ_RS03510 0.87 -0.9 658,553 + +0.2 658,595 - -0.4 658,596 + +0.9 658,635 - -1.0 658,643 - -1.1 658,646 - -0.2 658,648 - -0.3 658,649 + +0.2 658,674 - -1.6 658,675 + +0.6 658,675 + -0.7 658,692 - -0.7 658,693 + +0.5 658,693 + +0.9 658,698 - +1.3 658,698 - +0.4 658,698 - -0.4 658,698 - -0.7 658,698 - +2.9 658,699 + -2.6 658,699 + +0.0 658,699 + -1.7 658,699 + +0.2 658,700 - +1.0 658,700 - +1.0 658,700 - -1.1 658,701 + -0.8 658,701 + -0.2 658,701 + +0.1 658,701 + -1.2 658,701 + +1.8 658,701 + +0.5 658,701 + -0.5 658,701 + -0.6 658,703 + -0.5 658,712 - -0.5 658,740 - -0.7 658,741 + -1.2 658,741 + -0.1 658,760 + -0.3 658,770 + -0.3 658,866 - MMJJ_RS03515 0.20 -2.9 658,867 + MMJJ_RS03515 0.20 -0.9 658,893 + MMJJ_RS03515 0.25 +0.6 658,917 + MMJJ_RS03515 0.29 +0.2 658,928 - MMJJ_RS03515 0.30 +0.2 658,965 + MMJJ_RS03515 0.37 -1.3 658,971 - MMJJ_RS03515 0.38 +0.3 658,972 + MMJJ_RS03515 0.38 -2.5 658,978 - MMJJ_RS03515 0.39 -0.3 658,985 - MMJJ_RS03515 0.40 +0.3 659,087 - MMJJ_RS03515 0.57 +1.4 659,088 + MMJJ_RS03515 0.57 +0.3 659,144 - MMJJ_RS03515 0.67 -0.3 659,162 - MMJJ_RS03515 0.70 -0.1 659,162 - MMJJ_RS03515 0.70 -1.1 659,162 - MMJJ_RS03515 0.70 +2.6 659,165 - MMJJ_RS03515 0.70 +7.0 659,190 - MMJJ_RS03515 0.75 -0.2 659,190 - MMJJ_RS03515 0.75 +1.1 659,190 - MMJJ_RS03515 0.75 -0.4 659,190 - MMJJ_RS03515 0.75 +0.5 659,191 + MMJJ_RS03515 0.75 -0.6 659,210 - MMJJ_RS03515 0.78 +0.5 659,363 + +0.9 659,367 - +1.5 659,367 - +0.5 659,368 + -2.2 659,371 - +0.0 659,371 - -0.4 659,371 - +0.4 659,371 - +0.6 659,372 + -1.2 659,372 + -1.6 659,373 - +0.2 659,373 - +0.9 659,373 - -1.8 659,373 - -0.5 659,373 - -2.7 659,373 - -0.4 659,374 + -1.6 659,374 + +1.9 659,375 - +0.4 659,375 - -0.1 659,375 - +0.1 659,376 + +0.7 659,376 + +0.9 659,377 - -0.6 659,377 - -0.5 659,378 + -0.9 659,413 - +0.3 659,426 + -1.8 659,429 - -2.2 659,430 + -0.1 659,430 + -0.6 659,444 + -0.5 659,456 + +0.0 659,456 + +2.9 659,460 + -1.8 659,461 - +0.7 659,461 - -1.7 659,461 - -0.3 659,462 + MMJJ_RS03520 0.10 -2.4 659,462 + MMJJ_RS03520 0.10 -1.1 659,493 + MMJJ_RS03520 0.20 +0.5 659,493 + MMJJ_RS03520 0.20 -1.3 659,497 - MMJJ_RS03520 0.21 -1.8 659,498 + MMJJ_RS03520 0.22 +1.1 659,535 + MMJJ_RS03520 0.33 -0.3 659,554 + MMJJ_RS03520 0.39 +2.2 659,566 - MMJJ_RS03520 0.43 +1.2 659,566 - MMJJ_RS03520 0.43 -0.7 659,566 - MMJJ_RS03520 0.43 -0.9 659,566 - MMJJ_RS03520 0.43 -0.1 659,567 + MMJJ_RS03520 0.43 +0.2 659,567 + MMJJ_RS03520 0.43 +1.5 659,583 + MMJJ_RS03520 0.49 +0.5 659,585 + MMJJ_RS03520 0.49 +0.5 659,636 - MMJJ_RS03520 0.65 -0.5 659,636 - MMJJ_RS03520 0.65 +0.3 659,637 + MMJJ_RS03520 0.66 -1.6 659,642 - MMJJ_RS03520 0.67 -0.0 659,643 + MMJJ_RS03520 0.68 -0.5 659,643 + MMJJ_RS03520 0.68 +0.5 659,663 + MMJJ_RS03520 0.74 -0.4 659,698 - MMJJ_RS03520 0.85 -2.2 659,698 - MMJJ_RS03520 0.85 +0.2 659,698 - MMJJ_RS03520 0.85 -1.3 659,698 - MMJJ_RS03520 0.85 -0.9 659,698 - MMJJ_RS03520 0.85 -0.4 659,699 + MMJJ_RS03520 0.85 -0.7 659,699 + MMJJ_RS03520 0.85 -0.7 659,709 - MMJJ_RS03520 0.89 -1.0 659,709 - MMJJ_RS03520 0.89 -0.3 659,709 - MMJJ_RS03520 0.89 +0.7 659,709 - MMJJ_RS03520 0.89 -0.3 659,709 - MMJJ_RS03520 0.89 +0.5 659,710 + MMJJ_RS03520 0.89 -0.0 659,711 - MMJJ_RS03520 0.89 -1.1 659,711 - MMJJ_RS03520 0.89 -1.0 659,711 - MMJJ_RS03520 0.89 -0.6 659,712 + MMJJ_RS03520 0.90 +2.5 659,712 + MMJJ_RS03520 0.90 -1.3 659,712 + MMJJ_RS03520 0.90 -0.4 659,716 - -1.5 659,716 - +0.2 659,716 - +0.5 659,765 + +0.8 659,870 - -2.8 659,881 - -1.1 659,881 - -1.6 659,881 - +0.1 659,881 - +1.9 659,881 - +1.2 659,881 - -2.0 659,881 - +0.7 659,881 - -0.3 659,881 - -0.4 659,881 - -1.9 659,881 - -2.3 659,881 - +0.2 659,881 - +0.4 659,881 - +1.6 659,881 - -0.1 659,881 - -0.1 659,881 - -0.9 659,881 - +0.0 659,881 - -1.7 659,881 - -0.0 659,881 - +0.1 659,881 - +1.1 659,881 - +0.1 659,882 + -1.2 659,882 + -0.5 659,882 + -0.3 659,882 + +0.6 659,882 + +0.5 659,882 + +1.1 659,882 + +3.9 659,882 + -1.1 659,882 + -0.1 659,882 + +1.1 659,882 + -1.7 659,882 + -2.7 659,882 + -0.9 659,882 + -0.5 659,882 + -0.2 659,882 + -1.9 659,882 + -0.5 659,882 + -0.5 659,882 + -0.4 659,882 + +1.0 659,882 + -0.6 659,889 - -0.3 659,889 - +1.1 659,889 - +0.1 659,891 - +0.6 659,891 - -0.6 659,891 - -1.1 659,891 - -0.5 659,891 - -0.3 659,891 - +0.7 659,891 - +0.1 659,891 - +0.9 659,891 - +0.8 659,891 - +0.8 659,891 - +0.5 659,891 - -2.6 659,891 - +0.0 659,891 - +0.6 659,891 - +1.0 659,891 - +0.7 659,891 - -0.5 659,891 - +1.6 659,892 + -1.6 659,892 + +2.7 659,892 + -1.0 659,892 + -0.4 659,892 + +0.8 659,892 + -0.2 659,892 + -1.5 659,892 + -1.0 659,892 + +2.1 659,892 + +0.2 659,892 + +0.2 659,892 + -0.5 659,892 + -1.3 659,892 + -0.2 659,892 + +2.3 659,892 + -1.6 659,892 + -1.8 659,892 + +0.4 659,900 - -0.3 659,900 - +1.6 659,900 - -0.3 659,900 - +1.1 659,900 - +0.8 659,900 - -1.0 659,900 - +0.4 659,900 - +7.1 659,900 - -0.3 659,901 + +0.9 659,901 + +0.1 659,901 + -0.5 659,901 + -0.3 659,901 + -0.3 659,901 + -2.8 659,901 + -1.1 659,901 + -0.5 659,901 + +0.2 659,901 + -1.1 659,981 + -0.1 660,098 + MMJJ_RS03525 0.11 +0.3 660,149 - MMJJ_RS03525 0.16 -0.1 660,175 - MMJJ_RS03525 0.18 -0.3 660,276 + MMJJ_RS03525 0.27 +0.0 660,276 + MMJJ_RS03525 0.27 -0.4 660,334 - MMJJ_RS03525 0.32 +0.1 660,334 - MMJJ_RS03525 0.32 -1.1 660,334 - MMJJ_RS03525 0.32 -0.8 660,335 + MMJJ_RS03525 0.33 -0.3 660,335 + MMJJ_RS03525 0.33 -1.2 660,343 - MMJJ_RS03525 0.33 +6.9 660,344 + MMJJ_RS03525 0.33 +0.7 660,410 + MMJJ_RS03525 0.39 -0.3 660,437 - MMJJ_RS03525 0.42 +0.7 660,481 - MMJJ_RS03525 0.46 +3.1 660,481 - MMJJ_RS03525 0.46 -1.7 660,482 + MMJJ_RS03525 0.46 -1.4 660,511 - MMJJ_RS03525 0.48 +1.1 660,512 + MMJJ_RS03525 0.48 +1.1 660,573 - MMJJ_RS03525 0.54 -0.8 660,573 - MMJJ_RS03525 0.54 -0.5 660,586 - MMJJ_RS03525 0.55 -1.4 660,586 - MMJJ_RS03525 0.55 -1.9 660,586 - MMJJ_RS03525 0.55 -0.3 660,586 - MMJJ_RS03525 0.55 -0.1 660,587 + MMJJ_RS03525 0.55 -1.7 660,641 - MMJJ_RS03525 0.60 -2.2 660,658 + MMJJ_RS03525 0.61 +1.3 660,734 - MMJJ_RS03525 0.68 -0.2
Or see this region's nucleotide sequence