Experiment: N2 fixation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01120 and MMJJ_RS01125 are separated by 9 nucleotides MMJJ_RS01125 and MMJJ_RS09255 are separated by 170 nucleotides MMJJ_RS09255 and MMJJ_RS01135 are separated by 64 nucleotides MMJJ_RS01135 and MMJJ_RS01140 are separated by 308 nucleotides MMJJ_RS01140 and MMJJ_RS01150 are separated by 331 nucleotides
MMJJ_RS01120: MMJJ_RS01120 - hypothetical protein, at 217,540 to 217,821
_RS01120
MMJJ_RS01125: MMJJ_RS01125 - hypothetical protein, at 217,831 to 218,205
_RS01125
MMJJ_RS09255: MMJJ_RS09255 - hypothetical protein, at 218,376 to 218,561
_RS09255
MMJJ_RS01135: MMJJ_RS01135 - hypothetical protein, at 218,626 to 222,324
_RS01135
MMJJ_RS01140: MMJJ_RS01140 - helix-turn-helix domain-containing protein, at 222,633 to 222,836
_RS01140
MMJJ_RS01150: MMJJ_RS01150 - hypothetical protein, at 223,168 to 223,587
_RS01150
Position (kb) 218
219
220
221
222
223 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.627 kb on + strand, within MMJJ_RS01120 at 217.627 kb on + strand, within MMJJ_RS01120 at 217.628 kb on - strand, within MMJJ_RS01120 at 217.628 kb on - strand, within MMJJ_RS01120 at 217.667 kb on + strand, within MMJJ_RS01120 at 217.686 kb on - strand, within MMJJ_RS01120 at 217.686 kb on - strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.693 kb on - strand, within MMJJ_RS01120 at 217.694 kb on + strand, within MMJJ_RS01120 at 217.712 kb on + strand, within MMJJ_RS01120 at 217.712 kb on + strand, within MMJJ_RS01120 at 217.722 kb on + strand, within MMJJ_RS01120 at 217.733 kb on + strand, within MMJJ_RS01120 at 217.733 kb on + strand, within MMJJ_RS01120 at 217.774 kb on - strand, within MMJJ_RS01120 at 217.775 kb on + strand, within MMJJ_RS01120 at 217.780 kb on + strand, within MMJJ_RS01120 at 217.819 kb on - strand at 217.820 kb on + strand at 217.851 kb on - strand at 217.851 kb on - strand at 217.851 kb on - strand at 217.852 kb on + strand at 217.855 kb on + strand at 218.067 kb on + strand, within MMJJ_RS01125 at 218.089 kb on - strand, within MMJJ_RS01125 at 218.091 kb on - strand, within MMJJ_RS01125 at 218.094 kb on - strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.096 kb on - strand, within MMJJ_RS01125 at 218.144 kb on - strand, within MMJJ_RS01125 at 218.216 kb on + strand at 218.216 kb on + strand at 218.241 kb on - strand at 218.242 kb on + strand at 218.242 kb on + strand at 218.244 kb on - strand at 218.250 kb on - strand at 218.258 kb on - strand at 218.314 kb on - strand at 218.315 kb on + strand at 218.377 kb on - strand at 218.379 kb on - strand at 218.380 kb on + strand at 218.412 kb on + strand, within MMJJ_RS09255 at 218.414 kb on - strand, within MMJJ_RS09255 at 218.414 kb on - strand, within MMJJ_RS09255 at 218.442 kb on - strand, within MMJJ_RS09255 at 218.502 kb on + strand, within MMJJ_RS09255 at 218.634 kb on - strand at 218.653 kb on - strand at 218.654 kb on + strand at 218.659 kb on + strand at 218.675 kb on + strand at 218.677 kb on + strand at 218.677 kb on + strand at 218.682 kb on - strand at 218.731 kb on + strand at 218.914 kb on + strand at 218.917 kb on - strand at 219.034 kb on - strand, within MMJJ_RS01135 at 219.109 kb on + strand, within MMJJ_RS01135 at 219.121 kb on + strand, within MMJJ_RS01135 at 219.218 kb on - strand, within MMJJ_RS01135 at 219.219 kb on + strand, within MMJJ_RS01135 at 219.246 kb on - strand, within MMJJ_RS01135 at 219.246 kb on - strand, within MMJJ_RS01135 at 219.262 kb on - strand, within MMJJ_RS01135 at 219.264 kb on - strand, within MMJJ_RS01135 at 219.264 kb on - strand, within MMJJ_RS01135 at 219.283 kb on + strand, within MMJJ_RS01135 at 219.340 kb on - strand, within MMJJ_RS01135 at 219.346 kb on - strand, within MMJJ_RS01135 at 219.346 kb on - strand, within MMJJ_RS01135 at 219.346 kb on - strand, within MMJJ_RS01135 at 219.346 kb on - strand, within MMJJ_RS01135 at 219.346 kb on - strand, within MMJJ_RS01135 at 219.347 kb on + strand, within MMJJ_RS01135 at 219.347 kb on + strand, within MMJJ_RS01135 at 219.347 kb on + strand, within MMJJ_RS01135 at 219.379 kb on - strand at 219.380 kb on + strand, within MMJJ_RS01135 at 219.381 kb on - strand, within MMJJ_RS01135 at 219.381 kb on - strand, within MMJJ_RS01135 at 219.385 kb on + strand, within MMJJ_RS01135 at 219.508 kb on - strand, within MMJJ_RS01135 at 219.573 kb on + strand, within MMJJ_RS01135 at 219.643 kb on - strand, within MMJJ_RS01135 at 219.658 kb on + strand, within MMJJ_RS01135 at 219.660 kb on + strand, within MMJJ_RS01135 at 219.660 kb on + strand, within MMJJ_RS01135 at 219.700 kb on - strand, within MMJJ_RS01135 at 219.707 kb on + strand, within MMJJ_RS01135 at 219.740 kb on - strand, within MMJJ_RS01135 at 219.740 kb on - strand, within MMJJ_RS01135 at 219.753 kb on - strand, within MMJJ_RS01135 at 219.754 kb on + strand, within MMJJ_RS01135 at 219.804 kb on - strand, within MMJJ_RS01135 at 219.804 kb on - strand, within MMJJ_RS01135 at 219.827 kb on + strand, within MMJJ_RS01135 at 219.833 kb on + strand, within MMJJ_RS01135 at 219.872 kb on - strand, within MMJJ_RS01135 at 219.884 kb on - strand, within MMJJ_RS01135 at 219.884 kb on - strand, within MMJJ_RS01135 at 220.201 kb on - strand, within MMJJ_RS01135 at 220.202 kb on + strand, within MMJJ_RS01135 at 220.224 kb on - strand, within MMJJ_RS01135 at 220.225 kb on + strand, within MMJJ_RS01135 at 220.225 kb on + strand, within MMJJ_RS01135 at 220.331 kb on - strand, within MMJJ_RS01135 at 220.331 kb on - strand, within MMJJ_RS01135 at 220.404 kb on + strand, within MMJJ_RS01135 at 220.498 kb on + strand, within MMJJ_RS01135 at 220.498 kb on + strand, within MMJJ_RS01135 at 220.673 kb on - strand, within MMJJ_RS01135 at 220.673 kb on - strand, within MMJJ_RS01135 at 220.709 kb on - strand, within MMJJ_RS01135 at 220.714 kb on + strand, within MMJJ_RS01135 at 220.920 kb on - strand, within MMJJ_RS01135 at 220.984 kb on + strand, within MMJJ_RS01135 at 221.044 kb on - strand, within MMJJ_RS01135 at 221.045 kb on + strand, within MMJJ_RS01135 at 221.123 kb on - strand, within MMJJ_RS01135 at 221.284 kb on + strand, within MMJJ_RS01135 at 221.355 kb on + strand, within MMJJ_RS01135 at 221.535 kb on + strand, within MMJJ_RS01135 at 221.557 kb on + strand, within MMJJ_RS01135 at 221.596 kb on + strand, within MMJJ_RS01135 at 221.596 kb on + strand, within MMJJ_RS01135 at 221.610 kb on - strand, within MMJJ_RS01135 at 222.020 kb on - strand at 222.099 kb on - strand at 222.138 kb on + strand at 222.175 kb on + strand at 222.178 kb on + strand at 222.250 kb on + strand at 222.276 kb on - strand at 222.277 kb on + strand at 222.326 kb on - strand at 222.329 kb on - strand at 222.546 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.589 kb on + strand at 222.589 kb on + strand at 222.589 kb on + strand at 222.589 kb on + strand at 222.590 kb on - strand at 222.590 kb on - strand at 222.595 kb on - strand at 222.595 kb on - strand at 222.596 kb on + strand at 222.607 kb on - strand at 222.607 kb on - strand at 222.608 kb on + strand at 222.608 kb on + strand at 222.608 kb on + strand at 222.608 kb on + strand at 222.608 kb on + strand at 222.609 kb on - strand at 222.609 kb on - strand at 222.617 kb on - strand at 222.617 kb on - strand at 222.617 kb on - strand at 222.618 kb on + strand at 222.628 kb on - strand at 222.629 kb on + strand at 222.661 kb on - strand, within MMJJ_RS01140 at 222.662 kb on + strand, within MMJJ_RS01140 at 222.675 kb on - strand, within MMJJ_RS01140 at 222.675 kb on - strand, within MMJJ_RS01140 at 222.705 kb on - strand, within MMJJ_RS01140 at 222.708 kb on + strand, within MMJJ_RS01140 at 222.725 kb on - strand, within MMJJ_RS01140 at 222.737 kb on + strand, within MMJJ_RS01140 at 222.815 kb on + strand, within MMJJ_RS01140 at 222.815 kb on + strand, within MMJJ_RS01140 at 222.894 kb on - strand at 222.910 kb on - strand at 222.910 kb on - strand at 223.030 kb on - strand at 223.031 kb on + strand at 223.098 kb on + strand at 223.137 kb on - strand at 223.144 kb on - strand at 223.145 kb on + strand at 223.179 kb on - strand at 223.179 kb on - strand at 223.184 kb on + strand at 223.184 kb on + strand at 223.258 kb on - strand, within MMJJ_RS01150 at 223.259 kb on + strand, within MMJJ_RS01150 at 223.259 kb on + strand, within MMJJ_RS01150 at 223.270 kb on - strand, within MMJJ_RS01150
Per-strain Table
Position Strand Gene LocusTag Fraction N2 fixation, H2 media remove 217,626 - MMJJ_RS01120 0.30 +0.5 217,626 - MMJJ_RS01120 0.30 +1.0 217,626 - MMJJ_RS01120 0.30 -0.8 217,626 - MMJJ_RS01120 0.30 -0.9 217,626 - MMJJ_RS01120 0.30 +0.8 217,627 + MMJJ_RS01120 0.31 -0.8 217,627 + MMJJ_RS01120 0.31 -0.4 217,628 - MMJJ_RS01120 0.31 +0.1 217,628 - MMJJ_RS01120 0.31 +0.0 217,667 + MMJJ_RS01120 0.45 -0.9 217,686 - MMJJ_RS01120 0.52 +0.1 217,686 - MMJJ_RS01120 0.52 +2.1 217,687 + MMJJ_RS01120 0.52 -0.1 217,687 + MMJJ_RS01120 0.52 -1.2 217,687 + MMJJ_RS01120 0.52 +0.0 217,687 + MMJJ_RS01120 0.52 -0.8 217,693 - MMJJ_RS01120 0.54 -0.1 217,694 + MMJJ_RS01120 0.55 -0.4 217,712 + MMJJ_RS01120 0.61 +1.9 217,712 + MMJJ_RS01120 0.61 +0.4 217,722 + MMJJ_RS01120 0.65 -1.1 217,733 + MMJJ_RS01120 0.68 -0.0 217,733 + MMJJ_RS01120 0.68 +0.4 217,774 - MMJJ_RS01120 0.83 +1.5 217,775 + MMJJ_RS01120 0.83 -0.2 217,780 + MMJJ_RS01120 0.85 +2.9 217,819 - +0.7 217,820 + -0.7 217,851 - -0.5 217,851 - +1.1 217,851 - -0.1 217,852 + +1.1 217,855 + -0.9 218,067 + MMJJ_RS01125 0.63 -1.7 218,089 - MMJJ_RS01125 0.69 -0.1 218,091 - MMJJ_RS01125 0.69 +0.8 218,094 - MMJJ_RS01125 0.70 -1.8 218,095 + MMJJ_RS01125 0.70 +0.8 218,095 + MMJJ_RS01125 0.70 +0.5 218,095 + MMJJ_RS01125 0.70 -0.4 218,096 - MMJJ_RS01125 0.71 -0.5 218,144 - MMJJ_RS01125 0.83 +0.2 218,216 + -1.3 218,216 + -0.5 218,241 - -0.3 218,242 + -0.5 218,242 + -2.2 218,244 - -0.4 218,250 - +0.8 218,258 - +0.7 218,314 - -0.1 218,315 + +1.2 218,377 - +1.9 218,379 - -0.7 218,380 + +0.7 218,412 + MMJJ_RS09255 0.19 -1.8 218,414 - MMJJ_RS09255 0.20 -1.0 218,414 - MMJJ_RS09255 0.20 +0.2 218,442 - MMJJ_RS09255 0.35 +0.5 218,502 + MMJJ_RS09255 0.68 +1.4 218,634 - -2.5 218,653 - -1.5 218,654 + -1.9 218,659 + +3.8 218,675 + -0.4 218,677 + -1.4 218,677 + +0.1 218,682 - -0.5 218,731 + +0.2 218,914 + +0.7 218,917 - +1.5 219,034 - MMJJ_RS01135 0.11 +1.8 219,109 + MMJJ_RS01135 0.13 +0.5 219,121 + MMJJ_RS01135 0.13 +0.9 219,218 - MMJJ_RS01135 0.16 +0.6 219,219 + MMJJ_RS01135 0.16 +0.2 219,246 - MMJJ_RS01135 0.17 -0.9 219,246 - MMJJ_RS01135 0.17 -0.4 219,262 - MMJJ_RS01135 0.17 -1.6 219,264 - MMJJ_RS01135 0.17 -1.7 219,264 - MMJJ_RS01135 0.17 +0.8 219,283 + MMJJ_RS01135 0.18 +0.7 219,340 - MMJJ_RS01135 0.19 -0.4 219,346 - MMJJ_RS01135 0.19 +0.8 219,346 - MMJJ_RS01135 0.19 -0.1 219,346 - MMJJ_RS01135 0.19 +2.3 219,346 - MMJJ_RS01135 0.19 -0.1 219,346 - MMJJ_RS01135 0.19 -0.7 219,347 + MMJJ_RS01135 0.19 -0.6 219,347 + MMJJ_RS01135 0.19 +0.2 219,347 + MMJJ_RS01135 0.19 -0.3 219,379 - +2.9 219,380 + MMJJ_RS01135 0.20 +3.1 219,381 - MMJJ_RS01135 0.20 -0.8 219,381 - MMJJ_RS01135 0.20 -1.3 219,385 + MMJJ_RS01135 0.21 -0.3 219,508 - MMJJ_RS01135 0.24 -0.3 219,573 + MMJJ_RS01135 0.26 +0.3 219,643 - MMJJ_RS01135 0.27 +0.9 219,658 + MMJJ_RS01135 0.28 -1.0 219,660 + MMJJ_RS01135 0.28 -0.1 219,660 + MMJJ_RS01135 0.28 +0.1 219,700 - MMJJ_RS01135 0.29 -0.5 219,707 + MMJJ_RS01135 0.29 -1.6 219,740 - MMJJ_RS01135 0.30 +0.2 219,740 - MMJJ_RS01135 0.30 -2.3 219,753 - MMJJ_RS01135 0.30 -2.4 219,754 + MMJJ_RS01135 0.30 +0.6 219,804 - MMJJ_RS01135 0.32 -1.6 219,804 - MMJJ_RS01135 0.32 +0.2 219,827 + MMJJ_RS01135 0.32 +0.8 219,833 + MMJJ_RS01135 0.33 +0.6 219,872 - MMJJ_RS01135 0.34 +0.6 219,884 - MMJJ_RS01135 0.34 -0.4 219,884 - MMJJ_RS01135 0.34 -0.5 220,201 - MMJJ_RS01135 0.43 -2.5 220,202 + MMJJ_RS01135 0.43 -0.1 220,224 - MMJJ_RS01135 0.43 +0.8 220,225 + MMJJ_RS01135 0.43 +1.6 220,225 + MMJJ_RS01135 0.43 -0.3 220,331 - MMJJ_RS01135 0.46 +0.7 220,331 - MMJJ_RS01135 0.46 -2.0 220,404 + MMJJ_RS01135 0.48 +0.6 220,498 + MMJJ_RS01135 0.51 -1.7 220,498 + MMJJ_RS01135 0.51 +1.2 220,673 - MMJJ_RS01135 0.55 -2.5 220,673 - MMJJ_RS01135 0.55 +1.9 220,709 - MMJJ_RS01135 0.56 +0.1 220,714 + MMJJ_RS01135 0.56 +0.6 220,920 - MMJJ_RS01135 0.62 -1.5 220,984 + MMJJ_RS01135 0.64 -1.6 221,044 - MMJJ_RS01135 0.65 +2.3 221,045 + MMJJ_RS01135 0.65 -2.6 221,123 - MMJJ_RS01135 0.68 +0.4 221,284 + MMJJ_RS01135 0.72 +0.5 221,355 + MMJJ_RS01135 0.74 +1.6 221,535 + MMJJ_RS01135 0.79 -0.5 221,557 + MMJJ_RS01135 0.79 -1.9 221,596 + MMJJ_RS01135 0.80 -0.3 221,596 + MMJJ_RS01135 0.80 +1.9 221,610 - MMJJ_RS01135 0.81 -0.8 222,020 - +1.3 222,099 - +0.3 222,138 + +0.7 222,175 + +1.1 222,178 + -1.1 222,250 + +0.4 222,276 - +0.0 222,277 + +0.2 222,326 - -1.0 222,329 - -0.4 222,546 - -0.6 222,588 - -1.1 222,588 - +2.3 222,588 - -3.8 222,588 - +1.5 222,588 - +1.2 222,588 - +0.7 222,588 - +2.0 222,589 + -1.0 222,589 + +0.4 222,589 + +0.9 222,589 + -0.8 222,590 - +7.7 222,590 - +0.2 222,595 - -0.9 222,595 - +0.4 222,596 + -0.5 222,607 - +0.1 222,607 - +2.9 222,608 + -0.5 222,608 + +0.8 222,608 + -0.9 222,608 + -1.1 222,608 + +0.2 222,609 - -1.6 222,609 - -1.5 222,617 - +1.5 222,617 - -0.7 222,617 - -0.5 222,618 + -0.6 222,628 - -0.6 222,629 + -1.8 222,661 - MMJJ_RS01140 0.14 -1.7 222,662 + MMJJ_RS01140 0.14 -0.6 222,675 - MMJJ_RS01140 0.21 +0.2 222,675 - MMJJ_RS01140 0.21 -1.2 222,705 - MMJJ_RS01140 0.35 +0.5 222,708 + MMJJ_RS01140 0.37 +0.5 222,725 - MMJJ_RS01140 0.45 -2.0 222,737 + MMJJ_RS01140 0.51 -0.2 222,815 + MMJJ_RS01140 0.89 -0.7 222,815 + MMJJ_RS01140 0.89 +3.2 222,894 - -1.8 222,910 - +1.1 222,910 - -0.6 223,030 - -1.0 223,031 + -0.4 223,098 + +0.3 223,137 - +1.3 223,144 - +1.1 223,145 + -1.7 223,179 - -1.1 223,179 - +0.6 223,184 + -0.7 223,184 + -1.0 223,258 - MMJJ_RS01150 0.21 -1.1 223,259 + MMJJ_RS01150 0.22 -0.6 223,259 + MMJJ_RS01150 0.22 -1.2 223,270 - MMJJ_RS01150 0.24 -1.7
Or see this region's nucleotide sequence