Experiment: N2 fixation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00360 and MMJJ_RS00365 are separated by 31 nucleotides MMJJ_RS00365 and MMJJ_RS00370 are separated by 96 nucleotides
MMJJ_RS00360: MMJJ_RS00360 - adenosylhomocysteinase, at 68,397 to 69,644
_RS00360
MMJJ_RS00365: MMJJ_RS00365 - dihydroorotate dehydrogenase electron transfer subunit, at 69,676 to 70,443
_RS00365
MMJJ_RS00370: MMJJ_RS00370 - asparagine synthase (glutamine-hydrolyzing), at 70,540 to 72,081
_RS00370
Position (kb) 69
70
71 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8 at 68.679 kb on - strand, within MMJJ_RS00360 at 68.679 kb on - strand, within MMJJ_RS00360 at 68.983 kb on - strand, within MMJJ_RS00360 at 69.378 kb on - strand at 69.378 kb on - strand, within MMJJ_RS00360 at 69.519 kb on - strand, within MMJJ_RS00360 at 70.074 kb on + strand at 70.149 kb on - strand, within MMJJ_RS00365 at 70.150 kb on + strand, within MMJJ_RS00365 at 70.257 kb on - strand, within MMJJ_RS00365 at 70.395 kb on + strand at 70.411 kb on - strand at 70.445 kb on + strand at 70.463 kb on - strand at 70.463 kb on - strand at 70.467 kb on - strand at 70.468 kb on + strand at 70.472 kb on + strand at 70.476 kb on - strand at 70.481 kb on + strand at 70.481 kb on + strand at 70.483 kb on + strand at 70.483 kb on + strand at 70.625 kb on - strand at 71.103 kb on + strand, within MMJJ_RS00370
Per-strain Table
Position Strand Gene LocusTag Fraction N2 fixation, H2 media remove 68,679 - MMJJ_RS00360 0.23 +0.7 68,679 - MMJJ_RS00360 0.23 +1.3 68,983 - MMJJ_RS00360 0.47 +1.1 69,378 - +3.3 69,378 - MMJJ_RS00360 0.79 +1.1 69,519 - MMJJ_RS00360 0.90 +0.9 70,074 + +1.6 70,149 - MMJJ_RS00365 0.62 +8.0 70,150 + MMJJ_RS00365 0.62 +2.1 70,257 - MMJJ_RS00365 0.76 +0.6 70,395 + -0.4 70,411 - +0.2 70,445 + -0.6 70,463 - +0.2 70,463 - -1.1 70,467 - -0.2 70,468 + -0.6 70,472 + +1.1 70,476 - -0.8 70,481 + -1.2 70,481 + +0.3 70,483 + +0.7 70,483 + +0.2 70,625 - +1.9 71,103 + MMJJ_RS00370 0.37 +1.9
Or see this region's nucleotide sequence