Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS35730

Experiment: R2A with Nickel (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS35720 and RR42_RS35725 are separated by 7 nucleotidesRR42_RS35725 and RR42_RS35730 are separated by 222 nucleotidesRR42_RS35730 and RR42_RS35735 are separated by 165 nucleotidesRR42_RS35735 and RR42_RS35740 are separated by 54 nucleotides RR42_RS35720: RR42_RS35720 - flagellar basal body rod protein FlgC, at 3,464,228 to 3,464,635 _RS35720 RR42_RS35725: RR42_RS35725 - flagellar biosynthesis protein FlgB, at 3,464,643 to 3,465,053 _RS35725 RR42_RS35730: RR42_RS35730 - flagellar basal body P-ring biosynthesis protein FlgA, at 3,465,276 to 3,465,995 _RS35730 RR42_RS35735: RR42_RS35735 - flagellar biosynthesis protein FlgM, at 3,466,161 to 3,466,466 _RS35735 RR42_RS35740: RR42_RS35740 - flagellar biosynthesis protein FlgN, at 3,466,521 to 3,466,979 _RS35740 Position (kb) 3465 3466Strain fitness (log2 ratio) -2 -1 0 1 2at 3464.298 kb on + strand, within RR42_RS35720at 3464.298 kb on + strand, within RR42_RS35720at 3464.679 kb on - strandat 3464.942 kb on - strand, within RR42_RS35725at 3464.951 kb on + strand, within RR42_RS35725at 3464.951 kb on + strand, within RR42_RS35725at 3464.951 kb on + strand, within RR42_RS35725at 3465.197 kb on + strandat 3465.197 kb on + strandat 3465.203 kb on - strandat 3465.203 kb on - strandat 3465.203 kb on - strandat 3465.203 kb on - strandat 3465.271 kb on + strandat 3465.271 kb on + strandat 3465.271 kb on + strandat 3465.272 kb on - strandat 3465.272 kb on - strandat 3465.272 kb on - strandat 3465.272 kb on - strandat 3465.272 kb on - strandat 3465.272 kb on - strandat 3465.464 kb on - strand, within RR42_RS35730at 3465.591 kb on + strand, within RR42_RS35730at 3465.592 kb on - strand, within RR42_RS35730at 3465.624 kb on + strand, within RR42_RS35730at 3465.625 kb on - strand, within RR42_RS35730at 3465.627 kb on + strand, within RR42_RS35730at 3465.627 kb on + strand, within RR42_RS35730at 3465.627 kb on + strand, within RR42_RS35730at 3465.627 kb on + strand, within RR42_RS35730at 3465.628 kb on - strand, within RR42_RS35730at 3465.628 kb on - strand, within RR42_RS35730at 3465.628 kb on - strand, within RR42_RS35730at 3466.035 kb on + strandat 3466.036 kb on - strandat 3466.055 kb on + strandat 3466.055 kb on + strandat 3466.055 kb on + strandat 3466.055 kb on + strandat 3466.108 kb on - strandat 3466.108 kb on - strandat 3466.132 kb on - strandat 3466.480 kb on + strandat 3466.507 kb on - strandat 3466.511 kb on + strandat 3466.512 kb on - strandat 3466.586 kb on - strand, within RR42_RS35740at 3466.611 kb on - strand, within RR42_RS35740at 3466.611 kb on - strand, within RR42_RS35740at 3466.921 kb on - strand, within RR42_RS35740

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM
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3,464,298 + RR42_RS35720 0.17 +1.7
3,464,298 + RR42_RS35720 0.17 -0.3
3,464,679 - -0.3
3,464,942 - RR42_RS35725 0.73 +0.4
3,464,951 + RR42_RS35725 0.75 -0.9
3,464,951 + RR42_RS35725 0.75 +0.7
3,464,951 + RR42_RS35725 0.75 +0.9
3,465,197 + +2.7
3,465,197 + -1.3
3,465,203 - -0.2
3,465,203 - +0.3
3,465,203 - +0.1
3,465,203 - +0.7
3,465,271 + +0.4
3,465,271 + +0.5
3,465,271 + +0.5
3,465,272 - +0.1
3,465,272 - +0.3
3,465,272 - -1.1
3,465,272 - -1.7
3,465,272 - +0.5
3,465,272 - -1.3
3,465,464 - RR42_RS35730 0.26 +0.8
3,465,591 + RR42_RS35730 0.44 -0.6
3,465,592 - RR42_RS35730 0.44 +1.0
3,465,624 + RR42_RS35730 0.48 +0.2
3,465,625 - RR42_RS35730 0.48 +1.2
3,465,627 + RR42_RS35730 0.49 +0.5
3,465,627 + RR42_RS35730 0.49 +0.1
3,465,627 + RR42_RS35730 0.49 +0.7
3,465,627 + RR42_RS35730 0.49 -2.7
3,465,628 - RR42_RS35730 0.49 +0.7
3,465,628 - RR42_RS35730 0.49 +0.0
3,465,628 - RR42_RS35730 0.49 +0.7
3,466,035 + -0.3
3,466,036 - -0.3
3,466,055 + +0.5
3,466,055 + -0.0
3,466,055 + -0.5
3,466,055 + -0.0
3,466,108 - +0.6
3,466,108 - +0.2
3,466,132 - +0.1
3,466,480 + -0.9
3,466,507 - +0.1
3,466,511 + -0.2
3,466,512 - +0.1
3,466,586 - RR42_RS35740 0.14 -0.3
3,466,611 - RR42_RS35740 0.20 +0.2
3,466,611 - RR42_RS35740 0.20 +0.3
3,466,921 - RR42_RS35740 0.87 +0.2

Or see this region's nucleotide sequence