Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34270 and RR42_RS34275 are separated by 216 nucleotides RR42_RS34275 and RR42_RS34280 are separated by 25 nucleotides RR42_RS34280 and RR42_RS34285 are separated by 219 nucleotides
RR42_RS34270: RR42_RS34270 - Fis family transcriptional regulator, at 3,116,377 to 3,118,302
_RS34270
RR42_RS34275: RR42_RS34275 - virulence protein, at 3,118,519 to 3,118,926
_RS34275
RR42_RS34280: RR42_RS34280 - hypothetical protein, at 3,118,952 to 3,119,224
_RS34280
RR42_RS34285: RR42_RS34285 - alpha/beta hydrolase, at 3,119,444 to 3,120,400
_RS34285
Position (kb)
3118
3119
3120 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3118.121 kb on + strand at 3118.121 kb on + strand at 3118.121 kb on + strand at 3118.121 kb on + strand at 3118.121 kb on + strand at 3118.122 kb on - strand at 3118.122 kb on - strand at 3118.122 kb on - strand at 3118.122 kb on - strand at 3118.179 kb on - strand at 3118.399 kb on + strand at 3118.400 kb on - strand at 3118.449 kb on - strand at 3118.449 kb on - strand at 3118.532 kb on + strand at 3118.548 kb on + strand at 3118.548 kb on + strand at 3118.549 kb on - strand at 3118.549 kb on - strand at 3118.759 kb on - strand, within RR42_RS34275 at 3118.870 kb on + strand, within RR42_RS34275 at 3118.870 kb on + strand, within RR42_RS34275 at 3118.870 kb on + strand, within RR42_RS34275 at 3118.871 kb on - strand, within RR42_RS34275 at 3119.174 kb on + strand, within RR42_RS34280 at 3119.174 kb on + strand, within RR42_RS34280 at 3119.175 kb on - strand, within RR42_RS34280 at 3119.175 kb on - strand, within RR42_RS34280 at 3119.175 kb on - strand, within RR42_RS34280 at 3119.273 kb on + strand at 3119.273 kb on + strand at 3119.274 kb on - strand at 3119.274 kb on - strand at 3119.278 kb on + strand at 3119.279 kb on - strand at 3119.279 kb on - strand at 3119.414 kb on - strand at 3119.414 kb on - strand at 3119.516 kb on + strand at 3119.517 kb on - strand at 3119.633 kb on + strand, within RR42_RS34285 at 3119.633 kb on + strand, within RR42_RS34285 at 3119.634 kb on - strand, within RR42_RS34285 at 3119.634 kb on - strand, within RR42_RS34285 at 3119.717 kb on + strand, within RR42_RS34285 at 3119.718 kb on - strand, within RR42_RS34285 at 3119.742 kb on + strand, within RR42_RS34285 at 3119.742 kb on + strand, within RR42_RS34285 at 3119.743 kb on - strand, within RR42_RS34285 at 3119.759 kb on + strand, within RR42_RS34285 at 3119.759 kb on + strand, within RR42_RS34285 at 3119.759 kb on + strand, within RR42_RS34285 at 3119.759 kb on + strand, within RR42_RS34285 at 3119.760 kb on - strand, within RR42_RS34285 at 3119.760 kb on - strand, within RR42_RS34285 at 3119.760 kb on - strand, within RR42_RS34285 at 3119.943 kb on + strand, within RR42_RS34285 at 3119.944 kb on - strand, within RR42_RS34285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 3,118,121 + -0.2 3,118,121 + -0.4 3,118,121 + -0.1 3,118,121 + +0.4 3,118,121 + -1.2 3,118,122 - +1.1 3,118,122 - +0.5 3,118,122 - +0.9 3,118,122 - +0.5 3,118,179 - -0.6 3,118,399 + -0.2 3,118,400 - -0.8 3,118,449 - +0.7 3,118,449 - -0.2 3,118,532 + +1.2 3,118,548 + +0.4 3,118,548 + +0.5 3,118,549 - -0.4 3,118,549 - -0.6 3,118,759 - RR42_RS34275 0.59 +0.7 3,118,870 + RR42_RS34275 0.86 -0.5 3,118,870 + RR42_RS34275 0.86 -0.3 3,118,870 + RR42_RS34275 0.86 +0.8 3,118,871 - RR42_RS34275 0.86 +0.2 3,119,174 + RR42_RS34280 0.81 +1.3 3,119,174 + RR42_RS34280 0.81 +0.5 3,119,175 - RR42_RS34280 0.82 +0.4 3,119,175 - RR42_RS34280 0.82 -1.0 3,119,175 - RR42_RS34280 0.82 +0.1 3,119,273 + -0.4 3,119,273 + -0.5 3,119,274 - +0.4 3,119,274 - +1.4 3,119,278 + -0.6 3,119,279 - +0.2 3,119,279 - +0.4 3,119,414 - -0.5 3,119,414 - -2.5 3,119,516 + -1.2 3,119,517 - -0.4 3,119,633 + RR42_RS34285 0.20 +0.7 3,119,633 + RR42_RS34285 0.20 -0.3 3,119,634 - RR42_RS34285 0.20 +1.3 3,119,634 - RR42_RS34285 0.20 +0.6 3,119,717 + RR42_RS34285 0.29 +0.1 3,119,718 - RR42_RS34285 0.29 -0.2 3,119,742 + RR42_RS34285 0.31 +1.0 3,119,742 + RR42_RS34285 0.31 -0.5 3,119,743 - RR42_RS34285 0.31 +1.9 3,119,759 + RR42_RS34285 0.33 -0.6 3,119,759 + RR42_RS34285 0.33 -0.3 3,119,759 + RR42_RS34285 0.33 -1.0 3,119,759 + RR42_RS34285 0.33 -1.2 3,119,760 - RR42_RS34285 0.33 +0.8 3,119,760 - RR42_RS34285 0.33 -0.2 3,119,760 - RR42_RS34285 0.33 -0.9 3,119,943 + RR42_RS34285 0.52 +0.3 3,119,944 - RR42_RS34285 0.52 +0.3
Or see this region's nucleotide sequence