Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33895 and RR42_RS33900 overlap by 4 nucleotides RR42_RS33900 and RR42_RS33905 overlap by 7 nucleotides RR42_RS33905 and RR42_RS33910 are separated by 118 nucleotides
RR42_RS33895: RR42_RS33895 - membrane protein, at 3,032,826 to 3,033,743
_RS33895
RR42_RS33900: RR42_RS33900 - membrane protein, at 3,033,740 to 3,033,952
_RS33900
RR42_RS33905: RR42_RS33905 - transporter, at 3,033,946 to 3,036,018
_RS33905
RR42_RS33910: RR42_RS33910 - LysR family transcriptional regulator, at 3,036,137 to 3,037,030
_RS33910
Position (kb)
3033
3034
3035
3036
3037 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3033.161 kb on - strand, within RR42_RS33895 at 3033.172 kb on + strand, within RR42_RS33895 at 3033.173 kb on - strand, within RR42_RS33895 at 3033.223 kb on + strand, within RR42_RS33895 at 3033.224 kb on - strand, within RR42_RS33895 at 3033.649 kb on + strand, within RR42_RS33895 at 3033.649 kb on + strand, within RR42_RS33895 at 3033.650 kb on - strand, within RR42_RS33895 at 3033.650 kb on - strand, within RR42_RS33895 at 3033.650 kb on - strand, within RR42_RS33895 at 3033.792 kb on + strand, within RR42_RS33900 at 3033.792 kb on + strand, within RR42_RS33900 at 3033.792 kb on + strand, within RR42_RS33900 at 3033.801 kb on - strand, within RR42_RS33900 at 3033.835 kb on - strand, within RR42_RS33900 at 3033.927 kb on + strand, within RR42_RS33900 at 3033.948 kb on - strand at 3033.948 kb on - strand at 3034.010 kb on + strand at 3034.011 kb on - strand at 3034.562 kb on + strand, within RR42_RS33905 at 3034.661 kb on + strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.727 kb on + strand, within RR42_RS33905 at 3034.727 kb on + strand, within RR42_RS33905 at 3034.728 kb on - strand, within RR42_RS33905 at 3034.728 kb on - strand, within RR42_RS33905 at 3034.919 kb on + strand, within RR42_RS33905 at 3034.919 kb on + strand, within RR42_RS33905 at 3034.919 kb on + strand, within RR42_RS33905 at 3034.919 kb on + strand, within RR42_RS33905 at 3034.920 kb on - strand, within RR42_RS33905 at 3035.217 kb on + strand, within RR42_RS33905 at 3035.670 kb on + strand, within RR42_RS33905 at 3035.670 kb on + strand, within RR42_RS33905 at 3035.671 kb on - strand, within RR42_RS33905 at 3035.671 kb on - strand, within RR42_RS33905 at 3035.673 kb on - strand, within RR42_RS33905 at 3035.693 kb on + strand, within RR42_RS33905 at 3035.693 kb on + strand, within RR42_RS33905 at 3035.693 kb on + strand, within RR42_RS33905 at 3035.694 kb on - strand, within RR42_RS33905 at 3035.726 kb on + strand, within RR42_RS33905 at 3035.727 kb on - strand, within RR42_RS33905 at 3035.912 kb on + strand at 3035.945 kb on + strand at 3035.946 kb on - strand at 3035.946 kb on - strand at 3035.946 kb on - strand at 3036.013 kb on - strand at 3036.027 kb on + strand at 3036.045 kb on + strand at 3036.046 kb on - strand at 3036.058 kb on + strand at 3036.164 kb on + strand at 3036.165 kb on - strand at 3036.165 kb on - strand at 3036.335 kb on + strand, within RR42_RS33910 at 3036.336 kb on - strand, within RR42_RS33910 at 3036.629 kb on + strand, within RR42_RS33910 at 3036.630 kb on - strand, within RR42_RS33910 at 3036.952 kb on + strand at 3036.953 kb on - strand at 3036.972 kb on - strand at 3036.972 kb on - strand at 3036.975 kb on + strand at 3036.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 3,033,161 - RR42_RS33895 0.36 -0.1 3,033,172 + RR42_RS33895 0.38 -1.9 3,033,173 - RR42_RS33895 0.38 +0.4 3,033,223 + RR42_RS33895 0.43 -0.2 3,033,224 - RR42_RS33895 0.43 +0.3 3,033,649 + RR42_RS33895 0.90 -0.7 3,033,649 + RR42_RS33895 0.90 -0.2 3,033,650 - RR42_RS33895 0.90 +0.5 3,033,650 - RR42_RS33895 0.90 -1.6 3,033,650 - RR42_RS33895 0.90 -0.1 3,033,792 + RR42_RS33900 0.24 -0.7 3,033,792 + RR42_RS33900 0.24 -0.8 3,033,792 + RR42_RS33900 0.24 -0.1 3,033,801 - RR42_RS33900 0.29 -0.2 3,033,835 - RR42_RS33900 0.45 +0.5 3,033,927 + RR42_RS33900 0.88 -0.1 3,033,948 - -0.4 3,033,948 - +0.8 3,034,010 + -0.3 3,034,011 - +0.2 3,034,562 + RR42_RS33905 0.30 -0.2 3,034,661 + RR42_RS33905 0.34 +0.1 3,034,662 - RR42_RS33905 0.35 -0.4 3,034,662 - RR42_RS33905 0.35 -0.2 3,034,662 - RR42_RS33905 0.35 -0.1 3,034,662 - RR42_RS33905 0.35 -4.4 3,034,662 - RR42_RS33905 0.35 +0.2 3,034,662 - RR42_RS33905 0.35 -1.4 3,034,662 - RR42_RS33905 0.35 +0.7 3,034,727 + RR42_RS33905 0.38 -0.4 3,034,727 + RR42_RS33905 0.38 -0.4 3,034,728 - RR42_RS33905 0.38 -1.2 3,034,728 - RR42_RS33905 0.38 +0.2 3,034,919 + RR42_RS33905 0.47 +0.7 3,034,919 + RR42_RS33905 0.47 +0.2 3,034,919 + RR42_RS33905 0.47 -0.3 3,034,919 + RR42_RS33905 0.47 -0.6 3,034,920 - RR42_RS33905 0.47 +0.6 3,035,217 + RR42_RS33905 0.61 -0.4 3,035,670 + RR42_RS33905 0.83 -0.2 3,035,670 + RR42_RS33905 0.83 +0.3 3,035,671 - RR42_RS33905 0.83 -0.0 3,035,671 - RR42_RS33905 0.83 +0.6 3,035,673 - RR42_RS33905 0.83 +0.3 3,035,693 + RR42_RS33905 0.84 -0.1 3,035,693 + RR42_RS33905 0.84 +0.3 3,035,693 + RR42_RS33905 0.84 -0.3 3,035,694 - RR42_RS33905 0.84 +0.6 3,035,726 + RR42_RS33905 0.86 +0.0 3,035,727 - RR42_RS33905 0.86 +0.6 3,035,912 + -0.0 3,035,945 + +0.7 3,035,946 - -1.1 3,035,946 - -0.1 3,035,946 - -1.6 3,036,013 - +0.2 3,036,027 + +0.9 3,036,045 + +0.7 3,036,046 - -0.1 3,036,058 + +0.2 3,036,164 + -0.3 3,036,165 - -0.0 3,036,165 - +0.2 3,036,335 + RR42_RS33910 0.22 +0.8 3,036,336 - RR42_RS33910 0.22 +1.4 3,036,629 + RR42_RS33910 0.55 +0.9 3,036,630 - RR42_RS33910 0.55 +0.3 3,036,952 + -0.0 3,036,953 - +2.8 3,036,972 - -1.7 3,036,972 - +0.3 3,036,975 + +0.3 3,036,976 - -0.2
Or see this region's nucleotide sequence