Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33435 and RR42_RS33440 are separated by 34 nucleotides RR42_RS33440 and RR42_RS33445 are separated by 131 nucleotides RR42_RS33445 and RR42_RS33450 are separated by 72 nucleotides
RR42_RS33435: RR42_RS33435 - alkyl sulfatase, at 2,919,429 to 2,921,405
_RS33435
RR42_RS33440: RR42_RS33440 - LysR family transcriptional regulator, at 2,921,440 to 2,922,369
_RS33440
RR42_RS33445: RR42_RS33445 - haloacid dehalogenase, at 2,922,501 to 2,923,151
_RS33445
RR42_RS33450: RR42_RS33450 - alanine racemase, at 2,923,224 to 2,924,378
_RS33450
Position (kb)
2921
2922
2923 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.527 kb on + strand, within RR42_RS33435 at 2920.528 kb on - strand, within RR42_RS33435 at 2920.528 kb on - strand, within RR42_RS33435 at 2920.528 kb on - strand, within RR42_RS33435 at 2920.528 kb on - strand, within RR42_RS33435 at 2920.528 kb on - strand, within RR42_RS33435 at 2920.528 kb on - strand, within RR42_RS33435 at 2920.528 kb on - strand, within RR42_RS33435 at 2920.528 kb on - strand, within RR42_RS33435 at 2920.533 kb on + strand, within RR42_RS33435 at 2920.534 kb on - strand, within RR42_RS33435 at 2920.534 kb on - strand, within RR42_RS33435 at 2920.534 kb on - strand, within RR42_RS33435 at 2920.647 kb on + strand, within RR42_RS33435 at 2920.647 kb on + strand, within RR42_RS33435 at 2920.651 kb on - strand, within RR42_RS33435 at 2920.683 kb on + strand, within RR42_RS33435 at 2920.692 kb on + strand, within RR42_RS33435 at 2920.692 kb on + strand, within RR42_RS33435 at 2920.704 kb on + strand, within RR42_RS33435 at 2920.704 kb on + strand, within RR42_RS33435 at 2920.705 kb on - strand, within RR42_RS33435 at 2920.816 kb on - strand, within RR42_RS33435 at 2920.816 kb on - strand, within RR42_RS33435 at 2920.900 kb on + strand, within RR42_RS33435 at 2920.962 kb on + strand, within RR42_RS33435 at 2920.963 kb on - strand, within RR42_RS33435 at 2921.071 kb on - strand, within RR42_RS33435 at 2921.154 kb on + strand, within RR42_RS33435 at 2921.154 kb on + strand, within RR42_RS33435 at 2921.154 kb on + strand, within RR42_RS33435 at 2921.155 kb on - strand, within RR42_RS33435 at 2921.187 kb on + strand, within RR42_RS33435 at 2921.187 kb on + strand, within RR42_RS33435 at 2921.187 kb on + strand, within RR42_RS33435 at 2921.188 kb on - strand, within RR42_RS33435 at 2921.357 kb on + strand at 2921.358 kb on - strand at 2921.370 kb on + strand at 2921.503 kb on + strand at 2921.691 kb on + strand, within RR42_RS33440 at 2921.861 kb on + strand, within RR42_RS33440 at 2921.941 kb on - strand, within RR42_RS33440 at 2921.941 kb on - strand, within RR42_RS33440 at 2922.418 kb on + strand at 2922.419 kb on - strand at 2922.421 kb on + strand at 2922.421 kb on + strand at 2922.422 kb on - strand at 2922.422 kb on - strand at 2922.429 kb on - strand at 2922.500 kb on + strand at 2922.500 kb on + strand at 2922.500 kb on + strand at 2922.500 kb on + strand at 2922.501 kb on - strand at 2922.501 kb on - strand at 2922.501 kb on - strand at 2922.589 kb on + strand, within RR42_RS33445 at 2922.589 kb on + strand, within RR42_RS33445 at 2922.590 kb on - strand, within RR42_RS33445 at 2922.643 kb on - strand, within RR42_RS33445 at 2922.657 kb on + strand, within RR42_RS33445 at 2922.657 kb on + strand, within RR42_RS33445 at 2922.657 kb on + strand, within RR42_RS33445 at 2922.657 kb on + strand, within RR42_RS33445 at 2922.658 kb on - strand, within RR42_RS33445 at 2922.672 kb on + strand, within RR42_RS33445 at 2922.681 kb on + strand, within RR42_RS33445 at 2922.924 kb on + strand, within RR42_RS33445 at 2922.924 kb on + strand, within RR42_RS33445 at 2922.925 kb on - strand, within RR42_RS33445 at 2922.925 kb on - strand, within RR42_RS33445 at 2922.925 kb on - strand, within RR42_RS33445 at 2922.925 kb on - strand, within RR42_RS33445 at 2922.946 kb on - strand, within RR42_RS33445 at 2923.050 kb on + strand, within RR42_RS33445 at 2923.050 kb on + strand, within RR42_RS33445 at 2923.223 kb on + strand at 2923.223 kb on + strand at 2923.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 2,920,527 + RR42_RS33435 0.56 +1.0 2,920,527 + RR42_RS33435 0.56 +0.4 2,920,527 + RR42_RS33435 0.56 +0.4 2,920,527 + RR42_RS33435 0.56 +1.0 2,920,527 + RR42_RS33435 0.56 -1.5 2,920,527 + RR42_RS33435 0.56 -0.4 2,920,527 + RR42_RS33435 0.56 +0.3 2,920,527 + RR42_RS33435 0.56 +0.6 2,920,527 + RR42_RS33435 0.56 +2.9 2,920,527 + RR42_RS33435 0.56 -0.8 2,920,527 + RR42_RS33435 0.56 +1.0 2,920,528 - RR42_RS33435 0.56 -0.3 2,920,528 - RR42_RS33435 0.56 +0.6 2,920,528 - RR42_RS33435 0.56 -1.2 2,920,528 - RR42_RS33435 0.56 +0.1 2,920,528 - RR42_RS33435 0.56 -0.0 2,920,528 - RR42_RS33435 0.56 -0.3 2,920,528 - RR42_RS33435 0.56 -1.1 2,920,528 - RR42_RS33435 0.56 -1.2 2,920,533 + RR42_RS33435 0.56 -0.5 2,920,534 - RR42_RS33435 0.56 +0.2 2,920,534 - RR42_RS33435 0.56 +0.3 2,920,534 - RR42_RS33435 0.56 -0.2 2,920,647 + RR42_RS33435 0.62 -0.2 2,920,647 + RR42_RS33435 0.62 +0.1 2,920,651 - RR42_RS33435 0.62 +0.3 2,920,683 + RR42_RS33435 0.63 +0.4 2,920,692 + RR42_RS33435 0.64 +0.1 2,920,692 + RR42_RS33435 0.64 -0.1 2,920,704 + RR42_RS33435 0.64 +0.1 2,920,704 + RR42_RS33435 0.64 -0.9 2,920,705 - RR42_RS33435 0.65 -0.2 2,920,816 - RR42_RS33435 0.70 -0.4 2,920,816 - RR42_RS33435 0.70 +1.1 2,920,900 + RR42_RS33435 0.74 -0.4 2,920,962 + RR42_RS33435 0.78 -0.3 2,920,963 - RR42_RS33435 0.78 -0.6 2,921,071 - RR42_RS33435 0.83 -0.3 2,921,154 + RR42_RS33435 0.87 -0.3 2,921,154 + RR42_RS33435 0.87 +1.2 2,921,154 + RR42_RS33435 0.87 -0.5 2,921,155 - RR42_RS33435 0.87 -0.2 2,921,187 + RR42_RS33435 0.89 +0.3 2,921,187 + RR42_RS33435 0.89 -0.0 2,921,187 + RR42_RS33435 0.89 -0.1 2,921,188 - RR42_RS33435 0.89 +1.0 2,921,357 + -1.5 2,921,358 - -0.4 2,921,370 + -1.0 2,921,503 + +0.8 2,921,691 + RR42_RS33440 0.27 -2.0 2,921,861 + RR42_RS33440 0.45 -0.6 2,921,941 - RR42_RS33440 0.54 -0.1 2,921,941 - RR42_RS33440 0.54 -1.4 2,922,418 + -0.7 2,922,419 - -0.4 2,922,421 + +0.1 2,922,421 + -0.2 2,922,422 - -1.4 2,922,422 - -0.3 2,922,429 - -1.3 2,922,500 + -0.1 2,922,500 + +0.9 2,922,500 + -0.3 2,922,500 + +0.6 2,922,501 - +0.1 2,922,501 - -0.1 2,922,501 - -0.1 2,922,589 + RR42_RS33445 0.14 +0.3 2,922,589 + RR42_RS33445 0.14 -0.7 2,922,590 - RR42_RS33445 0.14 -0.3 2,922,643 - RR42_RS33445 0.22 -0.3 2,922,657 + RR42_RS33445 0.24 -0.4 2,922,657 + RR42_RS33445 0.24 +0.1 2,922,657 + RR42_RS33445 0.24 +0.3 2,922,657 + RR42_RS33445 0.24 +0.1 2,922,658 - RR42_RS33445 0.24 +0.7 2,922,672 + RR42_RS33445 0.26 -0.1 2,922,681 + RR42_RS33445 0.28 +0.2 2,922,924 + RR42_RS33445 0.65 +0.9 2,922,924 + RR42_RS33445 0.65 -1.7 2,922,925 - RR42_RS33445 0.65 +0.1 2,922,925 - RR42_RS33445 0.65 -0.8 2,922,925 - RR42_RS33445 0.65 +0.1 2,922,925 - RR42_RS33445 0.65 -0.7 2,922,946 - RR42_RS33445 0.68 +0.1 2,923,050 + RR42_RS33445 0.84 -0.4 2,923,050 + RR42_RS33445 0.84 -0.5 2,923,223 + +1.2 2,923,223 + +0.2 2,923,224 - +0.8
Or see this region's nucleotide sequence