Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30985 and RR42_RS30990 are separated by 250 nucleotides RR42_RS30990 and RR42_RS30995 are separated by 60 nucleotides RR42_RS30995 and RR42_RS31000 are separated by 26 nucleotides
RR42_RS30985: RR42_RS30985 - histone-like nucleoid-structuring protein, at 2,333,850 to 2,334,182
_RS30985
RR42_RS30990: RR42_RS30990 - membrane protein, at 2,334,433 to 2,334,831
_RS30990
RR42_RS30995: RR42_RS30995 - hypothetical protein, at 2,334,892 to 2,335,635
_RS30995
RR42_RS31000: RR42_RS31000 - glutathionylspermidine synthase, at 2,335,662 to 2,336,834
_RS31000
Position (kb)
2334
2335
2336 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2334.156 kb on + strand at 2334.156 kb on + strand at 2334.192 kb on - strand at 2334.243 kb on - strand at 2334.279 kb on + strand at 2334.434 kb on + strand at 2334.476 kb on - strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.709 kb on + strand, within RR42_RS30990 at 2334.709 kb on + strand, within RR42_RS30990 at 2334.710 kb on - strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.747 kb on - strand, within RR42_RS30990 at 2334.748 kb on + strand, within RR42_RS30990 at 2334.749 kb on - strand, within RR42_RS30990 at 2334.791 kb on + strand, within RR42_RS30990 at 2334.791 kb on + strand, within RR42_RS30990 at 2334.792 kb on - strand at 2334.792 kb on - strand at 2334.800 kb on + strand at 2334.801 kb on - strand at 2334.806 kb on + strand at 2334.807 kb on - strand at 2334.807 kb on - strand at 2334.807 kb on - strand at 2334.860 kb on + strand at 2334.860 kb on + strand at 2335.109 kb on + strand, within RR42_RS30995 at 2335.109 kb on + strand, within RR42_RS30995 at 2335.109 kb on + strand, within RR42_RS30995 at 2335.110 kb on - strand, within RR42_RS30995 at 2335.110 kb on - strand, within RR42_RS30995 at 2335.196 kb on + strand, within RR42_RS30995 at 2335.197 kb on - strand, within RR42_RS30995 at 2335.199 kb on + strand, within RR42_RS30995 at 2335.232 kb on + strand, within RR42_RS30995 at 2335.241 kb on + strand, within RR42_RS30995 at 2335.242 kb on - strand, within RR42_RS30995 at 2335.242 kb on - strand, within RR42_RS30995 at 2335.242 kb on - strand, within RR42_RS30995 at 2335.322 kb on + strand, within RR42_RS30995 at 2335.322 kb on + strand, within RR42_RS30995 at 2335.323 kb on - strand, within RR42_RS30995 at 2335.323 kb on - strand, within RR42_RS30995 at 2335.398 kb on - strand, within RR42_RS30995 at 2335.398 kb on - strand, within RR42_RS30995 at 2335.678 kb on + strand at 2335.767 kb on + strand at 2335.768 kb on - strand at 2335.783 kb on + strand, within RR42_RS31000 at 2335.784 kb on - strand, within RR42_RS31000 at 2335.813 kb on + strand, within RR42_RS31000 at 2335.813 kb on + strand, within RR42_RS31000 at 2335.814 kb on - strand, within RR42_RS31000 at 2335.823 kb on - strand, within RR42_RS31000 at 2335.823 kb on - strand, within RR42_RS31000 at 2335.826 kb on + strand, within RR42_RS31000 at 2335.826 kb on + strand, within RR42_RS31000 at 2335.826 kb on + strand, within RR42_RS31000 at 2335.827 kb on - strand, within RR42_RS31000 at 2335.827 kb on - strand, within RR42_RS31000 at 2335.827 kb on - strand, within RR42_RS31000 at 2335.843 kb on + strand, within RR42_RS31000 at 2335.927 kb on + strand, within RR42_RS31000 at 2335.928 kb on - strand, within RR42_RS31000 at 2336.089 kb on + strand, within RR42_RS31000 at 2336.126 kb on - strand, within RR42_RS31000 at 2336.162 kb on - strand, within RR42_RS31000 at 2336.162 kb on - strand, within RR42_RS31000 at 2336.162 kb on - strand, within RR42_RS31000 at 2336.367 kb on - strand, within RR42_RS31000 at 2336.448 kb on - strand, within RR42_RS31000 at 2336.464 kb on + strand, within RR42_RS31000 at 2336.464 kb on + strand, within RR42_RS31000 at 2336.465 kb on - strand, within RR42_RS31000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 2,334,156 + +0.3 2,334,156 + +0.7 2,334,192 - -0.5 2,334,243 - +1.5 2,334,279 + +0.4 2,334,434 + +0.0 2,334,476 - RR42_RS30990 0.11 -0.1 2,334,560 + RR42_RS30990 0.32 -0.2 2,334,560 + RR42_RS30990 0.32 +0.1 2,334,560 + RR42_RS30990 0.32 +0.1 2,334,709 + RR42_RS30990 0.69 +1.1 2,334,709 + RR42_RS30990 0.69 +0.2 2,334,710 - RR42_RS30990 0.69 +0.6 2,334,746 + RR42_RS30990 0.78 -1.4 2,334,746 + RR42_RS30990 0.78 -1.3 2,334,746 + RR42_RS30990 0.78 -0.0 2,334,747 - RR42_RS30990 0.79 -0.5 2,334,748 + RR42_RS30990 0.79 +0.1 2,334,749 - RR42_RS30990 0.79 +0.6 2,334,791 + RR42_RS30990 0.90 +0.6 2,334,791 + RR42_RS30990 0.90 +0.1 2,334,792 - -0.4 2,334,792 - -0.1 2,334,800 + -0.0 2,334,801 - +0.5 2,334,806 + -0.7 2,334,807 - +0.3 2,334,807 - -1.7 2,334,807 - +0.1 2,334,860 + -0.5 2,334,860 + -2.6 2,335,109 + RR42_RS30995 0.29 +0.2 2,335,109 + RR42_RS30995 0.29 +0.1 2,335,109 + RR42_RS30995 0.29 -0.0 2,335,110 - RR42_RS30995 0.29 +0.2 2,335,110 - RR42_RS30995 0.29 -0.1 2,335,196 + RR42_RS30995 0.41 +0.1 2,335,197 - RR42_RS30995 0.41 -1.0 2,335,199 + RR42_RS30995 0.41 +0.0 2,335,232 + RR42_RS30995 0.46 +2.2 2,335,241 + RR42_RS30995 0.47 +0.2 2,335,242 - RR42_RS30995 0.47 +0.4 2,335,242 - RR42_RS30995 0.47 -0.0 2,335,242 - RR42_RS30995 0.47 +0.1 2,335,322 + RR42_RS30995 0.58 +0.1 2,335,322 + RR42_RS30995 0.58 +0.3 2,335,323 - RR42_RS30995 0.58 +1.0 2,335,323 - RR42_RS30995 0.58 +0.0 2,335,398 - RR42_RS30995 0.68 +0.4 2,335,398 - RR42_RS30995 0.68 +1.0 2,335,678 + -0.5 2,335,767 + -0.4 2,335,768 - +0.6 2,335,783 + RR42_RS31000 0.10 +0.5 2,335,784 - RR42_RS31000 0.10 +0.3 2,335,813 + RR42_RS31000 0.13 +0.2 2,335,813 + RR42_RS31000 0.13 -1.3 2,335,814 - RR42_RS31000 0.13 +0.3 2,335,823 - RR42_RS31000 0.14 -0.7 2,335,823 - RR42_RS31000 0.14 -0.6 2,335,826 + RR42_RS31000 0.14 -1.2 2,335,826 + RR42_RS31000 0.14 -0.8 2,335,826 + RR42_RS31000 0.14 -0.1 2,335,827 - RR42_RS31000 0.14 +0.5 2,335,827 - RR42_RS31000 0.14 -0.1 2,335,827 - RR42_RS31000 0.14 -0.2 2,335,843 + RR42_RS31000 0.15 -0.2 2,335,927 + RR42_RS31000 0.23 +0.2 2,335,928 - RR42_RS31000 0.23 +0.8 2,336,089 + RR42_RS31000 0.36 +0.3 2,336,126 - RR42_RS31000 0.40 -0.1 2,336,162 - RR42_RS31000 0.43 -0.1 2,336,162 - RR42_RS31000 0.43 -0.4 2,336,162 - RR42_RS31000 0.43 +0.6 2,336,367 - RR42_RS31000 0.60 +1.3 2,336,448 - RR42_RS31000 0.67 -1.3 2,336,464 + RR42_RS31000 0.68 +0.0 2,336,464 + RR42_RS31000 0.68 -0.4 2,336,465 - RR42_RS31000 0.68 -0.2
Or see this region's nucleotide sequence