Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25200 and RR42_RS25205 are separated by 375 nucleotides RR42_RS25205 and RR42_RS25210 are separated by 172 nucleotides
RR42_RS25200: RR42_RS25200 - TetR family transcriptional regulator, at 995,493 to 996,071
_RS25200
RR42_RS25205: RR42_RS25205 - glucose 1-dehydrogenase, at 996,447 to 997,181
_RS25205
RR42_RS25210: RR42_RS25210 - acetyl-CoA acetyltransferase, at 997,354 to 998,538
_RS25210
Position (kb) 996
997
998 Strain fitness (log2 ratio) -2
-1
0
1 at 995.453 kb on + strand at 995.453 kb on + strand at 995.454 kb on - strand at 995.546 kb on + strand at 995.546 kb on + strand at 995.634 kb on + strand, within RR42_RS25200 at 995.634 kb on + strand, within RR42_RS25200 at 995.634 kb on + strand, within RR42_RS25200 at 995.635 kb on - strand, within RR42_RS25200 at 995.635 kb on - strand, within RR42_RS25200 at 995.636 kb on + strand, within RR42_RS25200 at 995.763 kb on + strand, within RR42_RS25200 at 995.764 kb on - strand, within RR42_RS25200 at 996.084 kb on + strand at 996.084 kb on + strand at 996.084 kb on + strand at 996.084 kb on + strand at 996.085 kb on - strand at 996.085 kb on - strand at 996.145 kb on + strand at 996.299 kb on + strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.618 kb on + strand, within RR42_RS25205 at 996.619 kb on - strand, within RR42_RS25205 at 996.619 kb on - strand, within RR42_RS25205 at 996.709 kb on + strand, within RR42_RS25205 at 996.872 kb on + strand, within RR42_RS25205 at 996.900 kb on + strand, within RR42_RS25205 at 996.901 kb on - strand, within RR42_RS25205 at 996.901 kb on - strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 997.026 kb on - strand, within RR42_RS25205 at 997.050 kb on + strand, within RR42_RS25205 at 997.050 kb on + strand, within RR42_RS25205 at 997.091 kb on + strand, within RR42_RS25205 at 997.105 kb on - strand, within RR42_RS25205 at 997.105 kb on - strand, within RR42_RS25205 at 997.111 kb on - strand at 997.179 kb on + strand at 997.205 kb on + strand at 997.206 kb on - strand at 997.322 kb on + strand at 997.323 kb on - strand at 997.408 kb on - strand at 997.519 kb on - strand, within RR42_RS25210 at 997.519 kb on - strand, within RR42_RS25210 at 997.688 kb on + strand, within RR42_RS25210 at 997.792 kb on + strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.793 kb on - strand, within RR42_RS25210 at 997.835 kb on - strand, within RR42_RS25210 at 997.860 kb on + strand, within RR42_RS25210 at 997.861 kb on - strand, within RR42_RS25210 at 997.861 kb on - strand, within RR42_RS25210 at 997.864 kb on + strand, within RR42_RS25210 at 997.864 kb on + strand, within RR42_RS25210 at 997.865 kb on - strand, within RR42_RS25210 at 997.888 kb on - strand, within RR42_RS25210 at 997.928 kb on + strand, within RR42_RS25210 at 997.928 kb on + strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.929 kb on - strand, within RR42_RS25210 at 997.931 kb on + strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.932 kb on - strand, within RR42_RS25210 at 997.998 kb on - strand, within RR42_RS25210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 995,453 + +0.1 995,453 + +0.7 995,454 - -0.2 995,546 + +0.6 995,546 + +0.6 995,634 + RR42_RS25200 0.24 -0.0 995,634 + RR42_RS25200 0.24 -0.0 995,634 + RR42_RS25200 0.24 -0.0 995,635 - RR42_RS25200 0.25 -0.5 995,635 - RR42_RS25200 0.25 -0.6 995,636 + RR42_RS25200 0.25 +0.1 995,763 + RR42_RS25200 0.47 -0.0 995,764 - RR42_RS25200 0.47 +0.8 996,084 + +0.7 996,084 + +0.7 996,084 + +0.3 996,084 + +0.4 996,085 - -0.2 996,085 - -0.3 996,145 + -0.1 996,299 + +0.7 996,355 - -2.1 996,355 - +0.1 996,355 - +0.1 996,355 - -0.3 996,618 + RR42_RS25205 0.23 +0.6 996,619 - RR42_RS25205 0.23 +0.3 996,619 - RR42_RS25205 0.23 -0.0 996,709 + RR42_RS25205 0.36 -0.0 996,872 + RR42_RS25205 0.58 +0.3 996,900 + RR42_RS25205 0.62 -0.8 996,901 - RR42_RS25205 0.62 +0.3 996,901 - RR42_RS25205 0.62 +0.1 996,992 + RR42_RS25205 0.74 -0.2 996,992 + RR42_RS25205 0.74 +1.0 996,992 + RR42_RS25205 0.74 +0.4 996,992 + RR42_RS25205 0.74 -0.8 996,993 - RR42_RS25205 0.74 -0.3 996,993 - RR42_RS25205 0.74 +0.7 996,993 - RR42_RS25205 0.74 -0.2 997,026 - RR42_RS25205 0.79 -0.5 997,050 + RR42_RS25205 0.82 +1.2 997,050 + RR42_RS25205 0.82 -0.2 997,091 + RR42_RS25205 0.88 +0.6 997,105 - RR42_RS25205 0.90 +0.3 997,105 - RR42_RS25205 0.90 +0.1 997,111 - +1.2 997,179 + -0.1 997,205 + +0.3 997,206 - +0.2 997,322 + -2.0 997,323 - -1.5 997,408 - +0.3 997,519 - RR42_RS25210 0.14 -0.2 997,519 - RR42_RS25210 0.14 -2.6 997,688 + RR42_RS25210 0.28 -1.0 997,792 + RR42_RS25210 0.37 +0.1 997,793 - RR42_RS25210 0.37 -0.9 997,793 - RR42_RS25210 0.37 +0.7 997,793 - RR42_RS25210 0.37 +0.2 997,835 - RR42_RS25210 0.41 -0.1 997,860 + RR42_RS25210 0.43 -0.1 997,861 - RR42_RS25210 0.43 +0.3 997,861 - RR42_RS25210 0.43 -0.3 997,864 + RR42_RS25210 0.43 -0.5 997,864 + RR42_RS25210 0.43 +0.5 997,865 - RR42_RS25210 0.43 +0.3 997,888 - RR42_RS25210 0.45 +1.0 997,928 + RR42_RS25210 0.48 -0.3 997,928 + RR42_RS25210 0.48 +0.0 997,929 - RR42_RS25210 0.49 -0.1 997,929 - RR42_RS25210 0.49 -0.7 997,929 - RR42_RS25210 0.49 -0.0 997,931 + RR42_RS25210 0.49 +0.2 997,932 - RR42_RS25210 0.49 -1.8 997,932 - RR42_RS25210 0.49 +0.3 997,932 - RR42_RS25210 0.49 +0.1 997,998 - RR42_RS25210 0.54 -0.0
Or see this region's nucleotide sequence