Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21610 and RR42_RS21615 are separated by 414 nucleotides RR42_RS21615 and RR42_RS21620 are separated by 20 nucleotides RR42_RS21620 and RR42_RS21625 are separated by 83 nucleotides
RR42_RS21610: RR42_RS21610 - MFS transporter permease, at 175,831 to 177,141
_RS21610
RR42_RS21615: RR42_RS21615 - alcohol dehydrogenase, at 177,556 to 180,558
_RS21615
RR42_RS21620: RR42_RS21620 - ferredoxin, at 180,579 to 181,085
_RS21620
RR42_RS21625: RR42_RS21625 - 6-hydroxynicotinate 3-monooxygenase, at 181,169 to 182,323
_RS21625
Position (kb) 177
178
179
180
181 Strain fitness (log2 ratio) -2
-1
0
1 at 176.715 kb on - strand, within RR42_RS21610 at 176.715 kb on - strand, within RR42_RS21610 at 176.715 kb on - strand, within RR42_RS21610 at 176.900 kb on - strand, within RR42_RS21610 at 176.916 kb on - strand, within RR42_RS21610 at 176.948 kb on + strand, within RR42_RS21610 at 176.949 kb on - strand, within RR42_RS21610 at 177.032 kb on + strand at 177.033 kb on - strand at 177.077 kb on + strand at 177.078 kb on - strand at 177.104 kb on - strand at 177.106 kb on + strand at 177.129 kb on - strand at 177.134 kb on + strand at 177.134 kb on + strand at 177.170 kb on + strand at 177.272 kb on - strand at 177.466 kb on + strand at 177.558 kb on - strand at 177.615 kb on + strand at 177.615 kb on + strand at 177.724 kb on - strand at 177.724 kb on - strand at 177.726 kb on - strand at 177.890 kb on + strand, within RR42_RS21615 at 178.010 kb on + strand, within RR42_RS21615 at 178.010 kb on + strand, within RR42_RS21615 at 178.011 kb on - strand, within RR42_RS21615 at 178.011 kb on - strand, within RR42_RS21615 at 178.217 kb on + strand, within RR42_RS21615 at 178.400 kb on + strand, within RR42_RS21615 at 178.401 kb on - strand, within RR42_RS21615 at 178.460 kb on + strand, within RR42_RS21615 at 178.466 kb on + strand, within RR42_RS21615 at 178.511 kb on + strand, within RR42_RS21615 at 178.511 kb on - strand, within RR42_RS21615 at 178.526 kb on + strand, within RR42_RS21615 at 178.634 kb on + strand, within RR42_RS21615 at 178.729 kb on + strand, within RR42_RS21615 at 178.730 kb on - strand, within RR42_RS21615 at 179.128 kb on + strand, within RR42_RS21615 at 179.390 kb on + strand, within RR42_RS21615 at 179.391 kb on - strand, within RR42_RS21615 at 179.413 kb on + strand, within RR42_RS21615 at 179.414 kb on - strand, within RR42_RS21615 at 179.732 kb on - strand, within RR42_RS21615 at 179.824 kb on - strand, within RR42_RS21615 at 179.824 kb on - strand, within RR42_RS21615 at 179.834 kb on + strand, within RR42_RS21615 at 179.835 kb on - strand, within RR42_RS21615 at 179.991 kb on - strand, within RR42_RS21615 at 180.036 kb on + strand, within RR42_RS21615 at 180.036 kb on + strand, within RR42_RS21615 at 180.037 kb on - strand, within RR42_RS21615 at 180.188 kb on + strand, within RR42_RS21615 at 180.388 kb on + strand at 180.388 kb on + strand at 180.388 kb on + strand at 180.388 kb on + strand at 180.389 kb on - strand at 180.797 kb on + strand, within RR42_RS21620 at 180.798 kb on - strand, within RR42_RS21620 at 180.958 kb on + strand, within RR42_RS21620 at 180.958 kb on + strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 181.039 kb on + strand at 181.040 kb on - strand at 181.040 kb on - strand at 181.040 kb on - strand at 181.273 kb on + strand at 181.274 kb on - strand at 181.424 kb on - strand, within RR42_RS21625 at 181.424 kb on - strand, within RR42_RS21625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 176,715 - RR42_RS21610 0.67 +0.0 176,715 - RR42_RS21610 0.67 -0.1 176,715 - RR42_RS21610 0.67 -0.2 176,900 - RR42_RS21610 0.82 +0.9 176,916 - RR42_RS21610 0.83 +0.4 176,948 + RR42_RS21610 0.85 +0.4 176,949 - RR42_RS21610 0.85 -0.4 177,032 + -0.5 177,033 - +0.1 177,077 + -0.1 177,078 - +1.4 177,104 - -0.1 177,106 + +0.5 177,129 - -0.7 177,134 + -0.0 177,134 + +0.2 177,170 + -0.3 177,272 - +1.7 177,466 + +0.2 177,558 - +0.3 177,615 + +0.2 177,615 + -0.3 177,724 - +1.1 177,724 - +0.3 177,726 - -0.6 177,890 + RR42_RS21615 0.11 -0.3 178,010 + RR42_RS21615 0.15 -0.6 178,010 + RR42_RS21615 0.15 +0.7 178,011 - RR42_RS21615 0.15 -0.1 178,011 - RR42_RS21615 0.15 +0.7 178,217 + RR42_RS21615 0.22 +1.6 178,400 + RR42_RS21615 0.28 -0.0 178,401 - RR42_RS21615 0.28 -0.1 178,460 + RR42_RS21615 0.30 +0.6 178,466 + RR42_RS21615 0.30 +0.8 178,511 + RR42_RS21615 0.32 +0.8 178,511 - RR42_RS21615 0.32 +1.7 178,526 + RR42_RS21615 0.32 +1.0 178,634 + RR42_RS21615 0.36 +0.0 178,729 + RR42_RS21615 0.39 +0.1 178,730 - RR42_RS21615 0.39 +0.8 179,128 + RR42_RS21615 0.52 -0.6 179,390 + RR42_RS21615 0.61 +0.1 179,391 - RR42_RS21615 0.61 +0.6 179,413 + RR42_RS21615 0.62 +1.5 179,414 - RR42_RS21615 0.62 -0.4 179,732 - RR42_RS21615 0.72 -0.2 179,824 - RR42_RS21615 0.76 -0.4 179,824 - RR42_RS21615 0.76 +1.1 179,834 + RR42_RS21615 0.76 +0.2 179,835 - RR42_RS21615 0.76 -0.5 179,991 - RR42_RS21615 0.81 -1.0 180,036 + RR42_RS21615 0.83 +0.2 180,036 + RR42_RS21615 0.83 +0.9 180,037 - RR42_RS21615 0.83 +0.5 180,188 + RR42_RS21615 0.88 +0.8 180,388 + +0.2 180,388 + +0.1 180,388 + +0.6 180,388 + -0.3 180,389 - +0.4 180,797 + RR42_RS21620 0.43 -0.2 180,798 - RR42_RS21620 0.43 +0.3 180,958 + RR42_RS21620 0.75 +1.2 180,958 + RR42_RS21620 0.75 +0.5 180,998 - RR42_RS21620 0.83 +1.4 180,998 - RR42_RS21620 0.83 -0.3 180,998 - RR42_RS21620 0.83 +0.1 181,039 + +0.4 181,040 - +0.5 181,040 - +0.1 181,040 - -0.2 181,273 + +0.4 181,274 - -1.8 181,424 - RR42_RS21625 0.22 +0.3 181,424 - RR42_RS21625 0.22 +0.1
Or see this region's nucleotide sequence