Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20865 and RR42_RS20870 are separated by 178 nucleotides RR42_RS20870 and RR42_RS20875 are separated by 499 nucleotides
RR42_RS20865: RR42_RS20865 - chromosome partitioning protein ParB, at 3,955 to 4,968
_RS20865
RR42_RS20870: RR42_RS20870 - integrase, at 5,147 to 6,730
_RS20870
RR42_RS20875: RR42_RS20875 - LuxR family transcriptional regulator, at 7,230 to 8,090
_RS20875
Position (kb) 5
6
7 Strain fitness (log2 ratio) -2
-1
0
1 at 4.969 kb on + strand at 4.970 kb on - strand at 4.970 kb on - strand at 4.984 kb on + strand at 4.985 kb on - strand at 4.985 kb on - strand at 4.985 kb on - strand at 6.787 kb on + strand at 6.803 kb on + strand at 6.939 kb on + strand at 6.939 kb on + strand at 6.940 kb on - strand at 6.972 kb on + strand at 7.102 kb on + strand at 7.102 kb on + strand at 7.136 kb on - strand at 7.157 kb on - strand at 7.209 kb on - strand at 7.342 kb on + strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.343 kb on - strand, within RR42_RS20875 at 7.348 kb on - strand, within RR42_RS20875 at 7.387 kb on - strand, within RR42_RS20875 at 7.691 kb on + strand, within RR42_RS20875 at 7.691 kb on + strand, within RR42_RS20875 at 7.692 kb on - strand, within RR42_RS20875 at 7.692 kb on - strand, within RR42_RS20875
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 4,969 + +0.5 4,970 - -0.3 4,970 - -1.1 4,984 + +0.8 4,985 - -0.2 4,985 - -2.1 4,985 - +0.1 6,787 + -1.0 6,803 + +0.2 6,939 + +0.2 6,939 + -0.3 6,940 - -0.3 6,972 + +1.4 7,102 + -1.0 7,102 + +0.6 7,136 - -1.1 7,157 - +0.0 7,209 - -1.5 7,342 + RR42_RS20875 0.13 +0.4 7,343 - RR42_RS20875 0.13 +0.0 7,343 - RR42_RS20875 0.13 +0.9 7,343 - RR42_RS20875 0.13 +1.0 7,343 - RR42_RS20875 0.13 -0.4 7,343 - RR42_RS20875 0.13 +0.2 7,343 - RR42_RS20875 0.13 -0.1 7,348 - RR42_RS20875 0.14 +1.0 7,387 - RR42_RS20875 0.18 +0.1 7,691 + RR42_RS20875 0.54 +0.1 7,691 + RR42_RS20875 0.54 +0.3 7,692 - RR42_RS20875 0.54 -1.4 7,692 - RR42_RS20875 0.54 -0.1
Or see this region's nucleotide sequence