Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16060 and RR42_RS16065 are separated by 224 nucleotides RR42_RS16065 and RR42_RS16070 overlap by 50 nucleotides RR42_RS16070 and RR42_RS16075 are separated by 199 nucleotides
RR42_RS16060: RR42_RS16060 - membrane protein, at 3,479,144 to 3,480,319
_RS16060
RR42_RS16065: RR42_RS16065 - GNAT family acetyltransferase, at 3,480,544 to 3,481,125
_RS16065
RR42_RS16070: RR42_RS16070 - cobyrinic acid a,c-diamide synthase, at 3,481,076 to 3,481,726
_RS16070
RR42_RS16075: RR42_RS16075 - cobyric acid synthase, at 3,481,926 to 3,483,386
_RS16075
Position (kb)
3480
3481
3482 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3479.719 kb on + strand, within RR42_RS16060 at 3479.719 kb on + strand, within RR42_RS16060 at 3479.720 kb on - strand, within RR42_RS16060 at 3479.880 kb on + strand, within RR42_RS16060 at 3480.028 kb on - strand, within RR42_RS16060 at 3480.038 kb on - strand, within RR42_RS16060 at 3480.087 kb on + strand, within RR42_RS16060 at 3480.087 kb on + strand, within RR42_RS16060 at 3480.152 kb on + strand, within RR42_RS16060 at 3480.153 kb on - strand, within RR42_RS16060 at 3480.153 kb on - strand, within RR42_RS16060 at 3480.274 kb on - strand at 3480.431 kb on + strand at 3480.431 kb on + strand at 3480.432 kb on - strand at 3480.631 kb on + strand, within RR42_RS16065 at 3480.631 kb on + strand, within RR42_RS16065 at 3480.631 kb on + strand, within RR42_RS16065 at 3480.632 kb on - strand, within RR42_RS16065 at 3480.632 kb on - strand, within RR42_RS16065 at 3480.805 kb on + strand, within RR42_RS16065 at 3480.806 kb on - strand, within RR42_RS16065 at 3480.806 kb on - strand, within RR42_RS16065 at 3480.814 kb on + strand, within RR42_RS16065 at 3480.814 kb on + strand, within RR42_RS16065 at 3480.815 kb on - strand, within RR42_RS16065 at 3480.815 kb on - strand, within RR42_RS16065 at 3480.931 kb on + strand, within RR42_RS16065 at 3480.931 kb on + strand, within RR42_RS16065 at 3480.932 kb on - strand, within RR42_RS16065 at 3480.932 kb on - strand, within RR42_RS16065 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.210 kb on - strand, within RR42_RS16070 at 3481.210 kb on - strand, within RR42_RS16070 at 3481.483 kb on + strand, within RR42_RS16070 at 3481.484 kb on - strand, within RR42_RS16070 at 3481.484 kb on - strand, within RR42_RS16070 at 3481.484 kb on - strand, within RR42_RS16070 at 3481.501 kb on + strand, within RR42_RS16070 at 3481.654 kb on - strand, within RR42_RS16070 at 3481.654 kb on - strand, within RR42_RS16070 at 3481.689 kb on - strand at 3481.719 kb on - strand at 3481.847 kb on + strand at 3481.848 kb on - strand at 3481.928 kb on - strand at 3481.928 kb on - strand at 3482.107 kb on + strand, within RR42_RS16075 at 3482.107 kb on + strand, within RR42_RS16075 at 3482.108 kb on - strand, within RR42_RS16075 at 3482.108 kb on - strand, within RR42_RS16075 at 3482.108 kb on - strand, within RR42_RS16075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 3,479,719 + RR42_RS16060 0.49 +0.2 3,479,719 + RR42_RS16060 0.49 +0.1 3,479,720 - RR42_RS16060 0.49 +0.2 3,479,880 + RR42_RS16060 0.63 -0.1 3,480,028 - RR42_RS16060 0.75 -0.8 3,480,038 - RR42_RS16060 0.76 +1.3 3,480,087 + RR42_RS16060 0.80 -0.7 3,480,087 + RR42_RS16060 0.80 -0.0 3,480,152 + RR42_RS16060 0.86 +0.9 3,480,153 - RR42_RS16060 0.86 -0.3 3,480,153 - RR42_RS16060 0.86 -2.7 3,480,274 - +0.0 3,480,431 + +0.1 3,480,431 + +0.3 3,480,432 - -0.4 3,480,631 + RR42_RS16065 0.15 -1.3 3,480,631 + RR42_RS16065 0.15 +1.7 3,480,631 + RR42_RS16065 0.15 +0.2 3,480,632 - RR42_RS16065 0.15 +0.2 3,480,632 - RR42_RS16065 0.15 +0.0 3,480,805 + RR42_RS16065 0.45 -0.6 3,480,806 - RR42_RS16065 0.45 -1.9 3,480,806 - RR42_RS16065 0.45 +0.0 3,480,814 + RR42_RS16065 0.46 -0.1 3,480,814 + RR42_RS16065 0.46 -0.8 3,480,815 - RR42_RS16065 0.47 +0.0 3,480,815 - RR42_RS16065 0.47 +0.2 3,480,931 + RR42_RS16065 0.66 +0.2 3,480,931 + RR42_RS16065 0.66 -2.2 3,480,932 - RR42_RS16065 0.67 -0.3 3,480,932 - RR42_RS16065 0.67 +0.2 3,481,209 + RR42_RS16070 0.20 -0.5 3,481,209 + RR42_RS16070 0.20 +0.1 3,481,209 + RR42_RS16070 0.20 -0.1 3,481,209 + RR42_RS16070 0.20 +0.7 3,481,209 + RR42_RS16070 0.20 +0.2 3,481,210 - RR42_RS16070 0.21 +0.4 3,481,210 - RR42_RS16070 0.21 -0.3 3,481,483 + RR42_RS16070 0.63 -2.3 3,481,484 - RR42_RS16070 0.63 -0.1 3,481,484 - RR42_RS16070 0.63 +0.0 3,481,484 - RR42_RS16070 0.63 +0.6 3,481,501 + RR42_RS16070 0.65 -0.4 3,481,654 - RR42_RS16070 0.89 -0.1 3,481,654 - RR42_RS16070 0.89 -0.2 3,481,689 - +0.4 3,481,719 - +2.6 3,481,847 + -0.3 3,481,848 - +0.5 3,481,928 - -0.1 3,481,928 - +1.7 3,482,107 + RR42_RS16075 0.12 -0.1 3,482,107 + RR42_RS16075 0.12 +0.3 3,482,108 - RR42_RS16075 0.12 -2.6 3,482,108 - RR42_RS16075 0.12 +0.6 3,482,108 - RR42_RS16075 0.12 -1.1
Or see this region's nucleotide sequence