Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15440 and RR42_RS15445 are separated by 65 nucleotides RR42_RS15445 and RR42_RS15450 are separated by 153 nucleotides RR42_RS15450 and RR42_RS15455 are separated by 18 nucleotides RR42_RS15455 and RR42_RS15460 are separated by 26 nucleotides
RR42_RS15440: RR42_RS15440 - transporter, at 3,336,696 to 3,337,811
_RS15440
RR42_RS15445: RR42_RS15445 - 7-cyano-7-deazaguanine synthase, at 3,337,877 to 3,338,560
_RS15445
RR42_RS15450: RR42_RS15450 - Tol system periplasmic component YbgF, at 3,338,714 to 3,339,472
_RS15450
RR42_RS15455: RR42_RS15455 - membrane protein, at 3,339,491 to 3,339,997
_RS15455
RR42_RS15460: RR42_RS15460 - translocation protein TolB, at 3,340,024 to 3,341,352
_RS15460
Position (kb)
3338
3339
3340 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3337.773 kb on + strand at 3337.774 kb on - strand at 3337.774 kb on - strand at 3337.774 kb on - strand at 3337.797 kb on - strand at 3337.894 kb on - strand at 3337.991 kb on - strand, within RR42_RS15445 at 3337.991 kb on - strand, within RR42_RS15445 at 3337.991 kb on - strand, within RR42_RS15445 at 3337.992 kb on + strand, within RR42_RS15445 at 3337.993 kb on - strand, within RR42_RS15445 at 3337.993 kb on - strand, within RR42_RS15445 at 3337.993 kb on - strand, within RR42_RS15445 at 3338.013 kb on + strand, within RR42_RS15445 at 3338.151 kb on + strand, within RR42_RS15445 at 3338.151 kb on + strand, within RR42_RS15445 at 3338.151 kb on + strand, within RR42_RS15445 at 3338.152 kb on - strand, within RR42_RS15445 at 3338.152 kb on - strand, within RR42_RS15445 at 3338.181 kb on + strand, within RR42_RS15445 at 3338.264 kb on - strand, within RR42_RS15445 at 3338.281 kb on - strand, within RR42_RS15445 at 3338.284 kb on + strand, within RR42_RS15445 at 3338.634 kb on + strand at 3338.635 kb on - strand at 3338.659 kb on - strand at 3338.659 kb on - strand at 3338.663 kb on + strand at 3338.664 kb on - strand at 3338.664 kb on - strand at 3338.673 kb on + strand at 3338.673 kb on + strand at 3338.674 kb on - strand at 3338.674 kb on - strand at 3338.715 kb on + strand at 3338.715 kb on + strand at 3338.716 kb on - strand at 3338.769 kb on + strand at 3338.769 kb on + strand at 3338.778 kb on - strand at 3338.778 kb on - strand at 3338.880 kb on + strand, within RR42_RS15450 at 3338.881 kb on - strand, within RR42_RS15450 at 3338.922 kb on + strand, within RR42_RS15450 at 3338.976 kb on + strand, within RR42_RS15450 at 3338.977 kb on - strand, within RR42_RS15450 at 3339.069 kb on + strand, within RR42_RS15450 at 3339.070 kb on - strand, within RR42_RS15450 at 3339.166 kb on - strand, within RR42_RS15450 at 3339.439 kb on + strand at 3339.439 kb on + strand at 3339.439 kb on + strand at 3339.492 kb on + strand at 3339.493 kb on - strand at 3339.493 kb on - strand at 3339.493 kb on - strand at 3339.495 kb on + strand at 3339.495 kb on + strand at 3339.495 kb on + strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.663 kb on + strand, within RR42_RS15455 at 3339.762 kb on + strand, within RR42_RS15455 at 3339.780 kb on + strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.798 kb on + strand, within RR42_RS15455 at 3339.799 kb on - strand, within RR42_RS15455 at 3339.892 kb on + strand, within RR42_RS15455 at 3339.997 kb on + strand at 3340.011 kb on - strand at 3340.026 kb on - strand at 3340.026 kb on - strand at 3340.319 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.419 kb on + strand, within RR42_RS15460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 3,337,773 + -1.3 3,337,774 - +0.1 3,337,774 - -0.5 3,337,774 - -1.1 3,337,797 - -0.7 3,337,894 - -0.6 3,337,991 - RR42_RS15445 0.17 -0.5 3,337,991 - RR42_RS15445 0.17 -0.0 3,337,991 - RR42_RS15445 0.17 +0.2 3,337,992 + RR42_RS15445 0.17 -0.4 3,337,993 - RR42_RS15445 0.17 +2.1 3,337,993 - RR42_RS15445 0.17 +0.1 3,337,993 - RR42_RS15445 0.17 -0.7 3,338,013 + RR42_RS15445 0.20 +0.2 3,338,151 + RR42_RS15445 0.40 -0.5 3,338,151 + RR42_RS15445 0.40 +1.3 3,338,151 + RR42_RS15445 0.40 -0.1 3,338,152 - RR42_RS15445 0.40 -0.5 3,338,152 - RR42_RS15445 0.40 -0.4 3,338,181 + RR42_RS15445 0.44 +0.2 3,338,264 - RR42_RS15445 0.57 +0.6 3,338,281 - RR42_RS15445 0.59 -0.4 3,338,284 + RR42_RS15445 0.60 -0.5 3,338,634 + +0.2 3,338,635 - +0.0 3,338,659 - +0.1 3,338,659 - -0.9 3,338,663 + -0.0 3,338,664 - +0.5 3,338,664 - -0.1 3,338,673 + -0.7 3,338,673 + +0.2 3,338,674 - +1.1 3,338,674 - +0.1 3,338,715 + -0.1 3,338,715 + +0.3 3,338,716 - -0.3 3,338,769 + +0.5 3,338,769 + +0.3 3,338,778 - -0.6 3,338,778 - -1.3 3,338,880 + RR42_RS15450 0.22 +0.3 3,338,881 - RR42_RS15450 0.22 -2.9 3,338,922 + RR42_RS15450 0.27 -2.0 3,338,976 + RR42_RS15450 0.35 -0.5 3,338,977 - RR42_RS15450 0.35 +0.5 3,339,069 + RR42_RS15450 0.47 +1.7 3,339,070 - RR42_RS15450 0.47 -0.0 3,339,166 - RR42_RS15450 0.60 -1.5 3,339,439 + -3.1 3,339,439 + -1.9 3,339,439 + -3.2 3,339,492 + -1.7 3,339,493 - -0.1 3,339,493 - +0.8 3,339,493 - +0.0 3,339,495 + -1.2 3,339,495 + -0.7 3,339,495 + -2.7 3,339,496 - -0.8 3,339,496 - +0.5 3,339,496 - -0.0 3,339,496 - +0.5 3,339,663 + RR42_RS15455 0.34 -0.9 3,339,762 + RR42_RS15455 0.53 -1.7 3,339,780 + RR42_RS15455 0.57 -1.5 3,339,781 - RR42_RS15455 0.57 -1.5 3,339,781 - RR42_RS15455 0.57 -1.1 3,339,781 - RR42_RS15455 0.57 -1.1 3,339,781 - RR42_RS15455 0.57 -2.5 3,339,781 - RR42_RS15455 0.57 -2.3 3,339,798 + RR42_RS15455 0.61 -4.9 3,339,799 - RR42_RS15455 0.61 -0.9 3,339,892 + RR42_RS15455 0.79 -1.1 3,339,997 + -1.9 3,340,011 - +0.0 3,340,026 - +0.0 3,340,026 - +0.4 3,340,319 + RR42_RS15460 0.22 +0.1 3,340,418 + RR42_RS15460 0.30 +0.7 3,340,418 + RR42_RS15460 0.30 -1.2 3,340,418 + RR42_RS15460 0.30 -0.7 3,340,419 + RR42_RS15460 0.30 -0.7
Or see this region's nucleotide sequence