Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS12835

Experiment: R2A with Nickel (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS12830 and RR42_RS12835 are separated by 149 nucleotidesRR42_RS12835 and RR42_RS12840 are separated by 117 nucleotidesRR42_RS12840 and RR42_RS12845 are separated by 63 nucleotides RR42_RS12830: RR42_RS12830 - multidrug resistance protein B, at 2,755,732 to 2,757,336 _RS12830 RR42_RS12835: RR42_RS12835 - tRNA pseudouridine synthase B, at 2,757,486 to 2,758,433 _RS12835 RR42_RS12840: RR42_RS12840 - ribosome-binding factor A, at 2,758,551 to 2,758,919 _RS12840 RR42_RS12845: RR42_RS12845 - translation initiation factor IF-2, at 2,758,983 to 2,761,883 _RS12845 Position (kb) 2757 2758 2759Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 2756.510 kb on + strand, within RR42_RS12830at 2756.511 kb on - strand, within RR42_RS12830at 2756.555 kb on - strand, within RR42_RS12830at 2756.598 kb on + strand, within RR42_RS12830at 2756.598 kb on + strand, within RR42_RS12830at 2756.599 kb on - strand, within RR42_RS12830at 2756.695 kb on + strand, within RR42_RS12830at 2756.696 kb on - strand, within RR42_RS12830at 2757.106 kb on + strand, within RR42_RS12830at 2757.106 kb on + strand, within RR42_RS12830at 2757.107 kb on - strand, within RR42_RS12830at 2757.118 kb on + strand, within RR42_RS12830at 2757.118 kb on + strand, within RR42_RS12830at 2757.119 kb on - strand, within RR42_RS12830at 2757.119 kb on - strand, within RR42_RS12830at 2757.119 kb on - strand, within RR42_RS12830at 2757.119 kb on - strand, within RR42_RS12830at 2757.119 kb on - strand, within RR42_RS12830at 2757.119 kb on - strand, within RR42_RS12830at 2757.120 kb on + strand, within RR42_RS12830at 2757.227 kb on + strandat 2757.228 kb on + strandat 2757.229 kb on - strandat 2757.292 kb on - strandat 2757.292 kb on - strandat 2757.335 kb on - strandat 2757.488 kb on - strandat 2757.568 kb on + strandat 2757.669 kb on + strand, within RR42_RS12835at 2757.869 kb on + strand, within RR42_RS12835at 2757.869 kb on + strand, within RR42_RS12835at 2757.870 kb on - strand, within RR42_RS12835at 2757.871 kb on + strand, within RR42_RS12835at 2757.871 kb on + strand, within RR42_RS12835at 2757.872 kb on - strand, within RR42_RS12835at 2757.872 kb on - strandat 2757.872 kb on - strand, within RR42_RS12835at 2757.872 kb on - strand, within RR42_RS12835at 2757.912 kb on + strand, within RR42_RS12835at 2757.913 kb on - strand, within RR42_RS12835at 2758.189 kb on + strand, within RR42_RS12835at 2758.189 kb on + strand, within RR42_RS12835at 2758.189 kb on + strand, within RR42_RS12835at 2758.190 kb on - strand, within RR42_RS12835at 2758.190 kb on - strand, within RR42_RS12835at 2758.190 kb on - strand, within RR42_RS12835at 2758.299 kb on + strand, within RR42_RS12835at 2758.419 kb on - strandat 2758.444 kb on + strandat 2758.444 kb on + strandat 2758.444 kb on + strandat 2758.444 kb on + strandat 2758.444 kb on + strandat 2758.445 kb on - strandat 2758.577 kb on + strandat 2758.925 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM
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2,756,510 + RR42_RS12830 0.48 -1.9
2,756,511 - RR42_RS12830 0.49 +1.1
2,756,555 - RR42_RS12830 0.51 +0.0
2,756,598 + RR42_RS12830 0.54 +0.4
2,756,598 + RR42_RS12830 0.54 -0.5
2,756,599 - RR42_RS12830 0.54 -1.0
2,756,695 + RR42_RS12830 0.60 -0.8
2,756,696 - RR42_RS12830 0.60 +0.4
2,757,106 + RR42_RS12830 0.86 -1.0
2,757,106 + RR42_RS12830 0.86 +0.4
2,757,107 - RR42_RS12830 0.86 -0.9
2,757,118 + RR42_RS12830 0.86 +0.2
2,757,118 + RR42_RS12830 0.86 -0.8
2,757,119 - RR42_RS12830 0.86 +5.0
2,757,119 - RR42_RS12830 0.86 +1.4
2,757,119 - RR42_RS12830 0.86 -0.9
2,757,119 - RR42_RS12830 0.86 -0.2
2,757,119 - RR42_RS12830 0.86 -0.1
2,757,119 - RR42_RS12830 0.86 -0.5
2,757,120 + RR42_RS12830 0.86 +0.8
2,757,227 + +0.2
2,757,228 + +0.2
2,757,229 - +0.0
2,757,292 - -0.1
2,757,292 - +0.7
2,757,335 - -0.8
2,757,488 - +1.4
2,757,568 + +0.0
2,757,669 + RR42_RS12835 0.19 +1.0
2,757,869 + RR42_RS12835 0.40 +0.2
2,757,869 + RR42_RS12835 0.40 -2.2
2,757,870 - RR42_RS12835 0.41 +0.5
2,757,871 + RR42_RS12835 0.41 +0.6
2,757,871 + RR42_RS12835 0.41 -0.2
2,757,872 - RR42_RS12835 0.41 +0.5
2,757,872 - +0.4
2,757,872 - RR42_RS12835 0.41 -0.3
2,757,872 - RR42_RS12835 0.41 +0.3
2,757,912 + RR42_RS12835 0.45 +0.8
2,757,913 - RR42_RS12835 0.45 +0.1
2,758,189 + RR42_RS12835 0.74 -0.8
2,758,189 + RR42_RS12835 0.74 +0.9
2,758,189 + RR42_RS12835 0.74 -0.0
2,758,190 - RR42_RS12835 0.74 -0.2
2,758,190 - RR42_RS12835 0.74 +0.4
2,758,190 - RR42_RS12835 0.74 +1.1
2,758,299 + RR42_RS12835 0.86 -0.0
2,758,419 - +0.4
2,758,444 + +0.4
2,758,444 + -1.0
2,758,444 + +0.5
2,758,444 + -0.3
2,758,444 + +0.0
2,758,445 - +0.1
2,758,577 + +0.4
2,758,925 - +0.1

Or see this region's nucleotide sequence