Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12130 and RR42_RS12135 are separated by 257 nucleotides RR42_RS12135 and RR42_RS12140 are separated by 236 nucleotides
RR42_RS12130: RR42_RS12130 - hypothetical protein, at 2,602,399 to 2,603,028
_RS12130
RR42_RS12135: RR42_RS12135 - hypothetical protein, at 2,603,286 to 2,605,466
_RS12135
RR42_RS12140: RR42_RS12140 - hypothetical protein, at 2,605,703 to 2,606,746
_RS12140
Position (kb)
2603
2604
2605
2606 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2602.303 kb on - strand at 2602.394 kb on + strand at 2602.394 kb on + strand at 2602.395 kb on - strand at 2602.395 kb on - strand at 2602.786 kb on + strand, within RR42_RS12130 at 2602.902 kb on + strand, within RR42_RS12130 at 2602.962 kb on - strand, within RR42_RS12130 at 2603.020 kb on + strand at 2603.065 kb on - strand at 2603.260 kb on + strand at 2603.260 kb on + strand at 2603.260 kb on + strand at 2603.290 kb on + strand at 2603.292 kb on + strand at 2603.344 kb on - strand at 2603.407 kb on - strand at 2603.407 kb on - strand at 2603.435 kb on + strand at 2603.435 kb on + strand at 2603.574 kb on + strand, within RR42_RS12135 at 2603.574 kb on + strand, within RR42_RS12135 at 2603.574 kb on + strand, within RR42_RS12135 at 2603.575 kb on - strand, within RR42_RS12135 at 2603.575 kb on - strand, within RR42_RS12135 at 2603.575 kb on - strand, within RR42_RS12135 at 2603.575 kb on - strand, within RR42_RS12135 at 2603.575 kb on - strand, within RR42_RS12135 at 2603.629 kb on - strand, within RR42_RS12135 at 2603.629 kb on - strand, within RR42_RS12135 at 2603.777 kb on + strand, within RR42_RS12135 at 2603.777 kb on + strand, within RR42_RS12135 at 2603.793 kb on + strand, within RR42_RS12135 at 2603.794 kb on - strand, within RR42_RS12135 at 2603.898 kb on + strand, within RR42_RS12135 at 2603.898 kb on + strand, within RR42_RS12135 at 2603.899 kb on - strand, within RR42_RS12135 at 2603.899 kb on - strand, within RR42_RS12135 at 2603.899 kb on - strand, within RR42_RS12135 at 2603.900 kb on + strand, within RR42_RS12135 at 2603.900 kb on + strand, within RR42_RS12135 at 2603.900 kb on + strand, within RR42_RS12135 at 2603.900 kb on + strand, within RR42_RS12135 at 2603.900 kb on + strand, within RR42_RS12135 at 2604.180 kb on + strand, within RR42_RS12135 at 2604.180 kb on + strand, within RR42_RS12135 at 2604.180 kb on + strand, within RR42_RS12135 at 2604.181 kb on - strand, within RR42_RS12135 at 2604.183 kb on - strand, within RR42_RS12135 at 2604.183 kb on - strand, within RR42_RS12135 at 2604.465 kb on + strand, within RR42_RS12135 at 2604.624 kb on + strand, within RR42_RS12135 at 2604.625 kb on - strand, within RR42_RS12135 at 2604.687 kb on + strand, within RR42_RS12135 at 2604.737 kb on + strand, within RR42_RS12135 at 2604.870 kb on + strand, within RR42_RS12135 at 2604.870 kb on + strand, within RR42_RS12135 at 2605.101 kb on + strand, within RR42_RS12135 at 2605.101 kb on + strand, within RR42_RS12135 at 2605.101 kb on + strand, within RR42_RS12135 at 2605.101 kb on + strand, within RR42_RS12135 at 2605.102 kb on - strand, within RR42_RS12135 at 2605.104 kb on + strand, within RR42_RS12135 at 2605.105 kb on - strand, within RR42_RS12135 at 2605.229 kb on - strand, within RR42_RS12135 at 2605.239 kb on - strand, within RR42_RS12135 at 2605.315 kb on + strand at 2605.316 kb on - strand at 2605.332 kb on + strand at 2605.332 kb on + strand at 2605.332 kb on + strand at 2605.333 kb on - strand at 2605.410 kb on + strand at 2605.411 kb on - strand at 2605.568 kb on - strand at 2605.660 kb on + strand at 2605.782 kb on + strand at 2605.783 kb on - strand at 2605.840 kb on - strand, within RR42_RS12140 at 2605.867 kb on - strand, within RR42_RS12140 at 2606.102 kb on - strand, within RR42_RS12140 at 2606.205 kb on + strand, within RR42_RS12140 at 2606.210 kb on - strand, within RR42_RS12140 at 2606.210 kb on - strand, within RR42_RS12140 at 2606.298 kb on + strand, within RR42_RS12140 at 2606.299 kb on - strand, within RR42_RS12140 at 2606.385 kb on + strand, within RR42_RS12140 at 2606.386 kb on - strand, within RR42_RS12140 at 2606.386 kb on - strand, within RR42_RS12140 at 2606.394 kb on + strand, within RR42_RS12140 at 2606.395 kb on - strand, within RR42_RS12140 at 2606.395 kb on - strand, within RR42_RS12140 at 2606.440 kb on - strand, within RR42_RS12140 at 2606.440 kb on - strand, within RR42_RS12140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 2,602,303 - +0.3 2,602,394 + +0.3 2,602,394 + +0.7 2,602,395 - -1.5 2,602,395 - -0.9 2,602,786 + RR42_RS12130 0.61 +0.1 2,602,902 + RR42_RS12130 0.80 +0.4 2,602,962 - RR42_RS12130 0.89 -1.0 2,603,020 + -0.8 2,603,065 - +1.1 2,603,260 + -0.6 2,603,260 + +0.7 2,603,260 + -0.2 2,603,290 + -0.1 2,603,292 + -0.4 2,603,344 - +0.1 2,603,407 - -0.7 2,603,407 - -0.2 2,603,435 + -0.2 2,603,435 + +0.0 2,603,574 + RR42_RS12135 0.13 +0.2 2,603,574 + RR42_RS12135 0.13 -0.5 2,603,574 + RR42_RS12135 0.13 +0.0 2,603,575 - RR42_RS12135 0.13 -0.2 2,603,575 - RR42_RS12135 0.13 -1.6 2,603,575 - RR42_RS12135 0.13 -0.1 2,603,575 - RR42_RS12135 0.13 +0.3 2,603,575 - RR42_RS12135 0.13 +0.4 2,603,629 - RR42_RS12135 0.16 +0.2 2,603,629 - RR42_RS12135 0.16 -0.4 2,603,777 + RR42_RS12135 0.23 +0.0 2,603,777 + RR42_RS12135 0.23 -0.0 2,603,793 + RR42_RS12135 0.23 +0.2 2,603,794 - RR42_RS12135 0.23 +0.1 2,603,898 + RR42_RS12135 0.28 -0.6 2,603,898 + RR42_RS12135 0.28 +0.0 2,603,899 - RR42_RS12135 0.28 +0.4 2,603,899 - RR42_RS12135 0.28 -0.4 2,603,899 - RR42_RS12135 0.28 +0.0 2,603,900 + RR42_RS12135 0.28 +0.1 2,603,900 + RR42_RS12135 0.28 -0.3 2,603,900 + RR42_RS12135 0.28 +0.1 2,603,900 + RR42_RS12135 0.28 +0.0 2,603,900 + RR42_RS12135 0.28 -0.0 2,604,180 + RR42_RS12135 0.41 +0.3 2,604,180 + RR42_RS12135 0.41 +1.8 2,604,180 + RR42_RS12135 0.41 -1.1 2,604,181 - RR42_RS12135 0.41 -0.9 2,604,183 - RR42_RS12135 0.41 +0.2 2,604,183 - RR42_RS12135 0.41 +0.2 2,604,465 + RR42_RS12135 0.54 +0.1 2,604,624 + RR42_RS12135 0.61 -1.0 2,604,625 - RR42_RS12135 0.61 -1.1 2,604,687 + RR42_RS12135 0.64 -0.6 2,604,737 + RR42_RS12135 0.67 +0.4 2,604,870 + RR42_RS12135 0.73 -1.0 2,604,870 + RR42_RS12135 0.73 -0.4 2,605,101 + RR42_RS12135 0.83 +0.5 2,605,101 + RR42_RS12135 0.83 +0.4 2,605,101 + RR42_RS12135 0.83 -0.2 2,605,101 + RR42_RS12135 0.83 +0.2 2,605,102 - RR42_RS12135 0.83 +0.6 2,605,104 + RR42_RS12135 0.83 -0.0 2,605,105 - RR42_RS12135 0.83 +0.1 2,605,229 - RR42_RS12135 0.89 -1.0 2,605,239 - RR42_RS12135 0.90 +0.3 2,605,315 + -0.7 2,605,316 - -0.1 2,605,332 + -0.0 2,605,332 + -1.6 2,605,332 + -0.1 2,605,333 - -1.8 2,605,410 + -0.2 2,605,411 - -0.0 2,605,568 - -0.4 2,605,660 + +0.7 2,605,782 + +0.2 2,605,783 - -0.0 2,605,840 - RR42_RS12140 0.13 -0.5 2,605,867 - RR42_RS12140 0.16 -0.6 2,606,102 - RR42_RS12140 0.38 +0.2 2,606,205 + RR42_RS12140 0.48 +0.3 2,606,210 - RR42_RS12140 0.49 +0.3 2,606,210 - RR42_RS12140 0.49 +1.4 2,606,298 + RR42_RS12140 0.57 -0.2 2,606,299 - RR42_RS12140 0.57 +1.1 2,606,385 + RR42_RS12140 0.65 -0.1 2,606,386 - RR42_RS12140 0.65 +1.2 2,606,386 - RR42_RS12140 0.65 -1.9 2,606,394 + RR42_RS12140 0.66 -0.0 2,606,395 - RR42_RS12140 0.66 -0.6 2,606,395 - RR42_RS12140 0.66 +0.0 2,606,440 - RR42_RS12140 0.71 -1.0 2,606,440 - RR42_RS12140 0.71 +0.1
Or see this region's nucleotide sequence