Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11045 and RR42_RS11050 are separated by 4 nucleotides RR42_RS11050 and RR42_RS11055 are separated by 65 nucleotides RR42_RS11055 and RR42_RS11060 are separated by 86 nucleotides
RR42_RS11045: RR42_RS11045 - short-chain dehydrogenase, at 2,356,999 to 2,357,820
_RS11045
RR42_RS11050: RR42_RS11050 - hypothetical protein, at 2,357,825 to 2,358,679
_RS11050
RR42_RS11055: RR42_RS11055 - 2-alkenal reductase, at 2,358,745 to 2,359,755
_RS11055
RR42_RS11060: RR42_RS11060 - 6-aminohexanoate hydrolase, at 2,359,842 to 2,361,161
_RS11060
Position (kb)
2358
2359
2360 Strain fitness (log2 ratio)
-2
-1
0
1 at 2357.865 kb on + strand at 2357.866 kb on - strand at 2357.866 kb on - strand at 2358.249 kb on + strand, within RR42_RS11050 at 2358.249 kb on + strand, within RR42_RS11050 at 2358.249 kb on + strand, within RR42_RS11050 at 2358.250 kb on - strand, within RR42_RS11050 at 2358.250 kb on - strand, within RR42_RS11050 at 2358.250 kb on - strand, within RR42_RS11050 at 2358.256 kb on + strand, within RR42_RS11050 at 2358.339 kb on + strand, within RR42_RS11050 at 2358.562 kb on + strand, within RR42_RS11050 at 2358.738 kb on + strand at 2358.739 kb on - strand at 2358.739 kb on - strand at 2358.739 kb on - strand at 2358.924 kb on + strand, within RR42_RS11055 at 2358.995 kb on + strand, within RR42_RS11055 at 2358.996 kb on - strand, within RR42_RS11055 at 2359.130 kb on + strand, within RR42_RS11055 at 2359.131 kb on - strand, within RR42_RS11055 at 2359.131 kb on - strand, within RR42_RS11055 at 2359.131 kb on - strand, within RR42_RS11055 at 2359.161 kb on - strand, within RR42_RS11055 at 2359.199 kb on + strand, within RR42_RS11055 at 2359.200 kb on - strand, within RR42_RS11055 at 2359.404 kb on + strand, within RR42_RS11055 at 2359.404 kb on + strand, within RR42_RS11055 at 2359.405 kb on - strand, within RR42_RS11055 at 2359.496 kb on + strand, within RR42_RS11055 at 2359.496 kb on + strand, within RR42_RS11055 at 2359.497 kb on - strand, within RR42_RS11055 at 2359.606 kb on - strand, within RR42_RS11055 at 2359.607 kb on + strand, within RR42_RS11055 at 2359.607 kb on + strand, within RR42_RS11055 at 2359.607 kb on + strand, within RR42_RS11055 at 2359.608 kb on - strand, within RR42_RS11055 at 2359.608 kb on - strand, within RR42_RS11055 at 2359.608 kb on - strand, within RR42_RS11055 at 2359.742 kb on + strand at 2359.743 kb on - strand at 2359.824 kb on + strand at 2359.824 kb on + strand at 2359.836 kb on - strand at 2359.836 kb on - strand at 2359.987 kb on + strand, within RR42_RS11060 at 2359.988 kb on - strand, within RR42_RS11060 at 2359.991 kb on + strand, within RR42_RS11060 at 2359.992 kb on - strand, within RR42_RS11060 at 2359.992 kb on - strand, within RR42_RS11060 at 2360.086 kb on + strand, within RR42_RS11060 at 2360.223 kb on - strand, within RR42_RS11060 at 2360.223 kb on - strand, within RR42_RS11060 at 2360.223 kb on - strand, within RR42_RS11060 at 2360.223 kb on - strand, within RR42_RS11060 at 2360.275 kb on + strand, within RR42_RS11060 at 2360.275 kb on + strand, within RR42_RS11060 at 2360.392 kb on + strand, within RR42_RS11060 at 2360.392 kb on + strand, within RR42_RS11060 at 2360.393 kb on - strand, within RR42_RS11060 at 2360.446 kb on + strand, within RR42_RS11060 at 2360.506 kb on + strand, within RR42_RS11060 at 2360.506 kb on + strand, within RR42_RS11060 at 2360.506 kb on + strand, within RR42_RS11060 at 2360.537 kb on - strand, within RR42_RS11060 at 2360.548 kb on + strand, within RR42_RS11060 at 2360.549 kb on - strand, within RR42_RS11060 at 2360.729 kb on + strand, within RR42_RS11060 at 2360.730 kb on - strand, within RR42_RS11060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 2,357,865 + -0.6 2,357,866 - +0.3 2,357,866 - +0.7 2,358,249 + RR42_RS11050 0.50 +0.5 2,358,249 + RR42_RS11050 0.50 +0.4 2,358,249 + RR42_RS11050 0.50 +1.1 2,358,250 - RR42_RS11050 0.50 +1.4 2,358,250 - RR42_RS11050 0.50 +0.7 2,358,250 - RR42_RS11050 0.50 -0.6 2,358,256 + RR42_RS11050 0.50 -0.5 2,358,339 + RR42_RS11050 0.60 -0.2 2,358,562 + RR42_RS11050 0.86 -0.2 2,358,738 + -0.7 2,358,739 - -1.9 2,358,739 - -0.7 2,358,739 - -0.4 2,358,924 + RR42_RS11055 0.18 +1.4 2,358,995 + RR42_RS11055 0.25 -0.4 2,358,996 - RR42_RS11055 0.25 -0.7 2,359,130 + RR42_RS11055 0.38 -0.2 2,359,131 - RR42_RS11055 0.38 -0.3 2,359,131 - RR42_RS11055 0.38 -0.6 2,359,131 - RR42_RS11055 0.38 -0.1 2,359,161 - RR42_RS11055 0.41 -0.1 2,359,199 + RR42_RS11055 0.45 +1.0 2,359,200 - RR42_RS11055 0.45 -0.7 2,359,404 + RR42_RS11055 0.65 +0.4 2,359,404 + RR42_RS11055 0.65 +0.3 2,359,405 - RR42_RS11055 0.65 -1.2 2,359,496 + RR42_RS11055 0.74 -0.6 2,359,496 + RR42_RS11055 0.74 -1.0 2,359,497 - RR42_RS11055 0.74 -0.4 2,359,606 - RR42_RS11055 0.85 +0.2 2,359,607 + RR42_RS11055 0.85 -0.5 2,359,607 + RR42_RS11055 0.85 -0.1 2,359,607 + RR42_RS11055 0.85 +0.5 2,359,608 - RR42_RS11055 0.85 -0.6 2,359,608 - RR42_RS11055 0.85 -0.1 2,359,608 - RR42_RS11055 0.85 -0.3 2,359,742 + +0.3 2,359,743 - -0.3 2,359,824 + -0.3 2,359,824 + +0.1 2,359,836 - -0.4 2,359,836 - -0.4 2,359,987 + RR42_RS11060 0.11 -1.3 2,359,988 - RR42_RS11060 0.11 -0.3 2,359,991 + RR42_RS11060 0.11 -0.5 2,359,992 - RR42_RS11060 0.11 -0.6 2,359,992 - RR42_RS11060 0.11 -1.2 2,360,086 + RR42_RS11060 0.18 -1.9 2,360,223 - RR42_RS11060 0.29 -1.1 2,360,223 - RR42_RS11060 0.29 -0.1 2,360,223 - RR42_RS11060 0.29 -0.6 2,360,223 - RR42_RS11060 0.29 +0.0 2,360,275 + RR42_RS11060 0.33 +0.1 2,360,275 + RR42_RS11060 0.33 -0.1 2,360,392 + RR42_RS11060 0.42 +0.4 2,360,392 + RR42_RS11060 0.42 -0.7 2,360,393 - RR42_RS11060 0.42 -0.2 2,360,446 + RR42_RS11060 0.46 +0.8 2,360,506 + RR42_RS11060 0.50 +0.1 2,360,506 + RR42_RS11060 0.50 -0.3 2,360,506 + RR42_RS11060 0.50 +1.4 2,360,537 - RR42_RS11060 0.53 +0.2 2,360,548 + RR42_RS11060 0.53 -0.4 2,360,549 - RR42_RS11060 0.54 -0.4 2,360,729 + RR42_RS11060 0.67 -0.1 2,360,730 - RR42_RS11060 0.67 +1.2
Or see this region's nucleotide sequence