Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05400 and RR42_RS05405 are separated by 107 nucleotides RR42_RS05405 and RR42_RS05410 are separated by 145 nucleotides
RR42_RS05400: RR42_RS05400 - GDP-mannose-dependent alpha-mannosyltransferase, at 1,192,903 to 1,194,069
_RS05400
RR42_RS05405: RR42_RS05405 - serine/threonine protein phosphatase, at 1,194,177 to 1,195,193
_RS05405
RR42_RS05410: RR42_RS05410 - acetyltransferase, at 1,195,339 to 1,196,316
_RS05410
Position (kb)
1194
1195
1196 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1193.311 kb on - strand, within RR42_RS05400 at 1193.351 kb on + strand, within RR42_RS05400 at 1193.351 kb on + strand, within RR42_RS05400 at 1193.351 kb on + strand, within RR42_RS05400 at 1193.351 kb on + strand, within RR42_RS05400 at 1193.352 kb on - strand, within RR42_RS05400 at 1193.380 kb on + strand, within RR42_RS05400 at 1193.381 kb on - strand, within RR42_RS05400 at 1193.391 kb on - strand, within RR42_RS05400 at 1193.391 kb on - strand, within RR42_RS05400 at 1193.520 kb on - strand, within RR42_RS05400 at 1193.553 kb on + strand, within RR42_RS05400 at 1193.554 kb on - strand, within RR42_RS05400 at 1193.702 kb on + strand, within RR42_RS05400 at 1193.703 kb on - strand, within RR42_RS05400 at 1193.741 kb on + strand, within RR42_RS05400 at 1194.069 kb on + strand at 1194.069 kb on + strand at 1194.069 kb on + strand at 1194.126 kb on + strand at 1194.134 kb on + strand at 1194.135 kb on - strand at 1194.135 kb on - strand at 1194.238 kb on + strand at 1194.238 kb on + strand at 1194.239 kb on - strand at 1194.239 kb on - strand at 1194.353 kb on + strand, within RR42_RS05405 at 1194.427 kb on + strand, within RR42_RS05405 at 1194.428 kb on - strand, within RR42_RS05405 at 1194.428 kb on - strand, within RR42_RS05405 at 1194.428 kb on - strand, within RR42_RS05405 at 1194.461 kb on - strand, within RR42_RS05405 at 1194.461 kb on - strand, within RR42_RS05405 at 1194.461 kb on - strand, within RR42_RS05405 at 1194.541 kb on + strand, within RR42_RS05405 at 1194.542 kb on - strand, within RR42_RS05405 at 1194.559 kb on + strand, within RR42_RS05405 at 1194.560 kb on - strand, within RR42_RS05405 at 1194.727 kb on + strand, within RR42_RS05405 at 1194.728 kb on - strand, within RR42_RS05405 at 1194.799 kb on + strand, within RR42_RS05405 at 1194.848 kb on - strand, within RR42_RS05405 at 1194.889 kb on + strand, within RR42_RS05405 at 1194.889 kb on + strand, within RR42_RS05405 at 1195.269 kb on + strand at 1195.269 kb on + strand at 1195.799 kb on + strand, within RR42_RS05410 at 1195.800 kb on - strand, within RR42_RS05410 at 1196.019 kb on - strand, within RR42_RS05410 at 1196.030 kb on + strand, within RR42_RS05410 at 1196.031 kb on - strand, within RR42_RS05410 at 1196.136 kb on + strand, within RR42_RS05410
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 1,193,311 - RR42_RS05400 0.35 -0.2 1,193,351 + RR42_RS05400 0.38 -1.2 1,193,351 + RR42_RS05400 0.38 +1.0 1,193,351 + RR42_RS05400 0.38 +0.1 1,193,351 + RR42_RS05400 0.38 +0.0 1,193,352 - RR42_RS05400 0.38 -0.6 1,193,380 + RR42_RS05400 0.41 +0.0 1,193,381 - RR42_RS05400 0.41 -2.7 1,193,391 - RR42_RS05400 0.42 +0.3 1,193,391 - RR42_RS05400 0.42 +0.0 1,193,520 - RR42_RS05400 0.53 -0.1 1,193,553 + RR42_RS05400 0.56 +0.1 1,193,554 - RR42_RS05400 0.56 +1.6 1,193,702 + RR42_RS05400 0.68 -0.2 1,193,703 - RR42_RS05400 0.69 -0.4 1,193,741 + RR42_RS05400 0.72 -0.4 1,194,069 + +0.1 1,194,069 + -0.3 1,194,069 + -0.2 1,194,126 + +0.1 1,194,134 + +0.2 1,194,135 - +0.1 1,194,135 - +0.9 1,194,238 + -0.4 1,194,238 + +2.0 1,194,239 - -0.6 1,194,239 - -0.6 1,194,353 + RR42_RS05405 0.17 -0.4 1,194,427 + RR42_RS05405 0.25 -1.2 1,194,428 - RR42_RS05405 0.25 -1.4 1,194,428 - RR42_RS05405 0.25 +0.3 1,194,428 - RR42_RS05405 0.25 -2.5 1,194,461 - RR42_RS05405 0.28 -0.2 1,194,461 - RR42_RS05405 0.28 -0.1 1,194,461 - RR42_RS05405 0.28 +0.4 1,194,541 + RR42_RS05405 0.36 -2.1 1,194,542 - RR42_RS05405 0.36 +0.0 1,194,559 + RR42_RS05405 0.38 -0.3 1,194,560 - RR42_RS05405 0.38 -0.9 1,194,727 + RR42_RS05405 0.54 +0.1 1,194,728 - RR42_RS05405 0.54 -2.3 1,194,799 + RR42_RS05405 0.61 +0.1 1,194,848 - RR42_RS05405 0.66 -0.6 1,194,889 + RR42_RS05405 0.70 -1.4 1,194,889 + RR42_RS05405 0.70 +0.4 1,195,269 + +0.2 1,195,269 + +0.4 1,195,799 + RR42_RS05410 0.47 +0.4 1,195,800 - RR42_RS05410 0.47 +0.1 1,196,019 - RR42_RS05410 0.70 +0.3 1,196,030 + RR42_RS05410 0.71 -0.4 1,196,031 - RR42_RS05410 0.71 +0.4 1,196,136 + RR42_RS05410 0.81 +1.5
Or see this region's nucleotide sequence