Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04710 and RR42_RS04715 are separated by 23 nucleotides RR42_RS04715 and RR42_RS04720 are separated by 15 nucleotides RR42_RS04720 and RR42_RS04725 are separated by 10 nucleotides
RR42_RS04710: RR42_RS04710 - 50S rRNA methyltransferase, at 1,049,443 to 1,049,913
_RS04710
RR42_RS04715: RR42_RS04715 - ribosome silencing factor RsfS, at 1,049,937 to 1,050,695
_RS04715
RR42_RS04720: RR42_RS04720 - nicotinate-nucleotide adenylyltransferase, at 1,050,711 to 1,051,481
_RS04720
RR42_RS04725: RR42_RS04725 - coproporphyrinogen III oxidase, at 1,051,492 to 1,052,403
_RS04725
Position (kb) 1050
1051
1052 Strain fitness (log2 ratio) -1
0
1 at 1049.772 kb on + strand, within RR42_RS04710 at 1049.772 kb on + strand, within RR42_RS04710 at 1049.773 kb on - strand, within RR42_RS04710 at 1049.773 kb on - strand, within RR42_RS04710 at 1050.173 kb on + strand, within RR42_RS04715 at 1050.403 kb on + strand, within RR42_RS04715 at 1050.403 kb on + strand, within RR42_RS04715 at 1050.404 kb on - strand, within RR42_RS04715 at 1050.404 kb on - strand, within RR42_RS04715 at 1050.406 kb on + strand, within RR42_RS04715 at 1050.407 kb on - strand, within RR42_RS04715 at 1050.407 kb on - strand, within RR42_RS04715 at 1050.622 kb on + strand at 1050.623 kb on - strand at 1050.623 kb on - strand at 1050.689 kb on + strand at 1050.690 kb on - strand at 1052.407 kb on - strand at 1052.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 1,049,772 + RR42_RS04710 0.70 -0.1 1,049,772 + RR42_RS04710 0.70 +0.9 1,049,773 - RR42_RS04710 0.70 -0.9 1,049,773 - RR42_RS04710 0.70 -0.2 1,050,173 + RR42_RS04715 0.31 -0.1 1,050,403 + RR42_RS04715 0.61 -0.3 1,050,403 + RR42_RS04715 0.61 -0.1 1,050,404 - RR42_RS04715 0.62 -0.6 1,050,404 - RR42_RS04715 0.62 -0.7 1,050,406 + RR42_RS04715 0.62 -0.3 1,050,407 - RR42_RS04715 0.62 -0.2 1,050,407 - RR42_RS04715 0.62 +1.2 1,050,622 + +0.1 1,050,623 - +0.2 1,050,623 - -0.0 1,050,689 + -0.4 1,050,690 - +0.8 1,052,407 - +0.3 1,052,407 - -0.7
Or see this region's nucleotide sequence