Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03420 and RR42_RS03425 are separated by 95 nucleotides RR42_RS03425 and RR42_RS03430 are separated by 104 nucleotides
RR42_RS03420: RR42_RS03420 - bile acid:sodium symporter, at 760,908 to 761,930
_RS03420
RR42_RS03425: RR42_RS03425 - LysR family transcriptional regulator, at 762,026 to 762,934
_RS03425
RR42_RS03430: RR42_RS03430 - SAM-dependent methyltransferase, at 763,039 to 763,797
_RS03430
Position (kb)
762
763 Strain fitness (log2 ratio)
-1
0
1
2 at 761.227 kb on + strand, within RR42_RS03420 at 761.227 kb on + strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.272 kb on + strand, within RR42_RS03420 at 761.273 kb on - strand, within RR42_RS03420 at 761.273 kb on - strand, within RR42_RS03420 at 761.426 kb on + strand, within RR42_RS03420 at 761.426 kb on + strand, within RR42_RS03420 at 761.427 kb on - strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.874 kb on + strand at 761.874 kb on + strand at 761.874 kb on + strand at 761.874 kb on + strand at 761.875 kb on - strand at 761.946 kb on + strand at 761.963 kb on - strand at 762.033 kb on - strand at 762.296 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.465 kb on + strand, within RR42_RS03425 at 762.465 kb on + strand, within RR42_RS03425 at 762.466 kb on - strand, within RR42_RS03425 at 762.597 kb on + strand, within RR42_RS03425 at 762.597 kb on + strand, within RR42_RS03425 at 762.598 kb on - strand, within RR42_RS03425 at 762.891 kb on + strand at 762.891 kb on + strand at 762.891 kb on + strand at 762.892 kb on - strand at 762.952 kb on + strand at 762.968 kb on + strand at 762.976 kb on + strand at 763.017 kb on - strand at 763.024 kb on + strand at 763.040 kb on + strand at 763.103 kb on + strand at 763.114 kb on + strand at 763.114 kb on + strand at 763.283 kb on + strand, within RR42_RS03430 at 763.293 kb on + strand, within RR42_RS03430 at 763.406 kb on + strand, within RR42_RS03430 at 763.592 kb on + strand, within RR42_RS03430 at 763.593 kb on - strand, within RR42_RS03430 at 763.593 kb on - strand, within RR42_RS03430 at 763.745 kb on + strand at 763.893 kb on - strand at 763.897 kb on + strand at 763.897 kb on + strand at 763.897 kb on + strand at 763.897 kb on + strand at 763.898 kb on - strand at 763.898 kb on - strand at 763.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 761,227 + RR42_RS03420 0.31 +0.9 761,227 + RR42_RS03420 0.31 +0.6 761,228 - RR42_RS03420 0.31 -1.3 761,228 - RR42_RS03420 0.31 -0.3 761,228 - RR42_RS03420 0.31 -0.1 761,272 + RR42_RS03420 0.36 +1.6 761,273 - RR42_RS03420 0.36 -0.3 761,273 - RR42_RS03420 0.36 +0.5 761,426 + RR42_RS03420 0.51 -0.8 761,426 + RR42_RS03420 0.51 +0.2 761,427 - RR42_RS03420 0.51 -0.1 761,590 + RR42_RS03420 0.67 -0.1 761,590 + RR42_RS03420 0.67 +1.2 761,590 + RR42_RS03420 0.67 +0.2 761,591 - RR42_RS03420 0.67 +0.7 761,591 - RR42_RS03420 0.67 -0.1 761,591 - RR42_RS03420 0.67 +0.9 761,591 - RR42_RS03420 0.67 -0.1 761,591 - RR42_RS03420 0.67 -0.8 761,674 + RR42_RS03420 0.75 +0.1 761,674 + RR42_RS03420 0.75 +0.6 761,674 + RR42_RS03420 0.75 +0.5 761,674 + RR42_RS03420 0.75 +1.5 761,674 + RR42_RS03420 0.75 -0.3 761,675 - RR42_RS03420 0.75 -0.2 761,675 - RR42_RS03420 0.75 -0.1 761,675 - RR42_RS03420 0.75 +0.9 761,675 - RR42_RS03420 0.75 +0.5 761,874 + +0.4 761,874 + -1.1 761,874 + +0.7 761,874 + -0.5 761,875 - -0.3 761,946 + +0.8 761,963 - +0.2 762,033 - -0.1 762,296 + RR42_RS03425 0.30 +0.3 762,356 + RR42_RS03425 0.36 +0.0 762,356 + RR42_RS03425 0.36 -0.1 762,356 + RR42_RS03425 0.36 -0.3 762,356 + RR42_RS03425 0.36 -0.4 762,356 + RR42_RS03425 0.36 -0.1 762,356 + RR42_RS03425 0.36 +0.8 762,357 - RR42_RS03425 0.36 -0.6 762,357 - RR42_RS03425 0.36 -0.1 762,357 - RR42_RS03425 0.36 -0.5 762,357 - RR42_RS03425 0.36 +0.2 762,357 - RR42_RS03425 0.36 -0.2 762,357 - RR42_RS03425 0.36 +0.8 762,357 - RR42_RS03425 0.36 -0.2 762,357 - RR42_RS03425 0.36 +0.1 762,357 - RR42_RS03425 0.36 -1.1 762,465 + RR42_RS03425 0.48 +0.2 762,465 + RR42_RS03425 0.48 +0.4 762,466 - RR42_RS03425 0.48 -0.3 762,597 + RR42_RS03425 0.63 +0.9 762,597 + RR42_RS03425 0.63 -0.1 762,598 - RR42_RS03425 0.63 -0.0 762,891 + +0.1 762,891 + +0.2 762,891 + -1.1 762,892 - -0.4 762,952 + +0.5 762,968 + +0.2 762,976 + -1.0 763,017 - -0.5 763,024 + +0.1 763,040 + +0.7 763,103 + -1.5 763,114 + +2.4 763,114 + +1.3 763,283 + RR42_RS03430 0.32 +0.4 763,293 + RR42_RS03430 0.33 +0.2 763,406 + RR42_RS03430 0.48 +0.7 763,592 + RR42_RS03430 0.73 -0.8 763,593 - RR42_RS03430 0.73 -0.1 763,593 - RR42_RS03430 0.73 +0.6 763,745 + -0.6 763,893 - -0.0 763,897 + -0.1 763,897 + -0.4 763,897 + -0.3 763,897 + +0.0 763,898 - +0.1 763,898 - -1.6 763,898 - -1.4
Or see this region's nucleotide sequence