Experiment: LB, pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2343 and Ac3H11_2344 overlap by 23 nucleotides Ac3H11_2344 and Ac3H11_2345 are separated by 66 nucleotides Ac3H11_2345 and Ac3H11_2346 are separated by 142 nucleotides
Ac3H11_2343 - FIG00978337: hypothetical protein, at 598,768 to 599,256
_2343
Ac3H11_2344 - Transcriptional activator MetR, at 599,234 to 600,160
_2344
Ac3H11_2345 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 600,227 to 602,659
_2345
Ac3H11_2346 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 602,802 to 603,755
_2346
Position (kb)
600
601
602
603 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 599.236 kb on - strand at 599.236 kb on - strand at 599.300 kb on + strand at 599.300 kb on + strand at 599.300 kb on + strand at 599.300 kb on + strand at 599.301 kb on - strand at 599.301 kb on - strand at 599.301 kb on - strand at 599.304 kb on + strand at 599.304 kb on + strand at 599.305 kb on - strand at 599.305 kb on - strand at 599.378 kb on - strand, within Ac3H11_2344 at 599.378 kb on - strand, within Ac3H11_2344 at 599.388 kb on + strand, within Ac3H11_2344 at 599.388 kb on + strand, within Ac3H11_2344 at 599.403 kb on + strand, within Ac3H11_2344 at 599.562 kb on + strand, within Ac3H11_2344 at 599.562 kb on + strand, within Ac3H11_2344 at 599.563 kb on - strand, within Ac3H11_2344 at 599.673 kb on + strand, within Ac3H11_2344 at 599.673 kb on + strand, within Ac3H11_2344 at 599.673 kb on + strand, within Ac3H11_2344 at 599.673 kb on + strand, within Ac3H11_2344 at 600.292 kb on - strand at 600.516 kb on + strand, within Ac3H11_2345 at 600.516 kb on + strand, within Ac3H11_2345 at 600.517 kb on - strand, within Ac3H11_2345 at 600.517 kb on - strand, within Ac3H11_2345 at 600.593 kb on + strand, within Ac3H11_2345 at 600.725 kb on + strand, within Ac3H11_2345 at 600.725 kb on + strand, within Ac3H11_2345 at 600.726 kb on - strand, within Ac3H11_2345 at 600.726 kb on - strand, within Ac3H11_2345 at 600.731 kb on + strand, within Ac3H11_2345 at 600.731 kb on + strand, within Ac3H11_2345 at 600.731 kb on + strand, within Ac3H11_2345 at 600.732 kb on - strand, within Ac3H11_2345 at 600.929 kb on + strand, within Ac3H11_2345 at 601.002 kb on + strand, within Ac3H11_2345 at 601.088 kb on + strand, within Ac3H11_2345 at 601.088 kb on + strand, within Ac3H11_2345 at 601.088 kb on + strand, within Ac3H11_2345 at 601.089 kb on - strand, within Ac3H11_2345 at 601.089 kb on - strand, within Ac3H11_2345 at 601.263 kb on + strand at 601.264 kb on - strand, within Ac3H11_2345 at 601.267 kb on + strand, within Ac3H11_2345 at 601.267 kb on + strand, within Ac3H11_2345 at 601.267 kb on + strand, within Ac3H11_2345 at 601.267 kb on + strand, within Ac3H11_2345 at 601.267 kb on + strand, within Ac3H11_2345 at 601.267 kb on + strand, within Ac3H11_2345 at 601.268 kb on - strand, within Ac3H11_2345 at 601.268 kb on - strand, within Ac3H11_2345 at 601.268 kb on - strand, within Ac3H11_2345 at 601.268 kb on - strand, within Ac3H11_2345 at 601.745 kb on + strand, within Ac3H11_2345 at 601.745 kb on + strand, within Ac3H11_2345 at 601.745 kb on + strand, within Ac3H11_2345 at 601.745 kb on + strand, within Ac3H11_2345 at 601.746 kb on - strand, within Ac3H11_2345 at 601.746 kb on - strand, within Ac3H11_2345 at 601.746 kb on - strand, within Ac3H11_2345 at 601.746 kb on - strand, within Ac3H11_2345 at 601.746 kb on - strand, within Ac3H11_2345 at 601.746 kb on - strand, within Ac3H11_2345 at 601.746 kb on - strand, within Ac3H11_2345 at 601.837 kb on + strand, within Ac3H11_2345 at 601.854 kb on - strand, within Ac3H11_2345 at 601.854 kb on - strand, within Ac3H11_2345 at 601.886 kb on - strand, within Ac3H11_2345 at 601.910 kb on + strand, within Ac3H11_2345 at 601.910 kb on + strand, within Ac3H11_2345 at 601.910 kb on + strand, within Ac3H11_2345 at 601.911 kb on - strand, within Ac3H11_2345 at 601.911 kb on - strand, within Ac3H11_2345 at 601.911 kb on - strand, within Ac3H11_2345 at 601.911 kb on - strand, within Ac3H11_2345 at 601.944 kb on - strand, within Ac3H11_2345 at 601.991 kb on + strand, within Ac3H11_2345 at 601.991 kb on + strand, within Ac3H11_2345 at 602.225 kb on + strand, within Ac3H11_2345 at 602.225 kb on + strand, within Ac3H11_2345 at 602.225 kb on + strand, within Ac3H11_2345 at 602.226 kb on - strand, within Ac3H11_2345 at 602.226 kb on - strand, within Ac3H11_2345 at 602.307 kb on - strand, within Ac3H11_2345 at 602.307 kb on - strand, within Ac3H11_2345 at 602.307 kb on - strand, within Ac3H11_2345 at 602.447 kb on + strand at 602.447 kb on + strand at 602.447 kb on + strand at 602.690 kb on + strand at 602.774 kb on - strand at 602.774 kb on - strand at 602.791 kb on - strand at 602.803 kb on + strand at 602.803 kb on + strand at 602.804 kb on - strand at 602.903 kb on - strand, within Ac3H11_2346 at 602.903 kb on - strand, within Ac3H11_2346 at 602.965 kb on + strand, within Ac3H11_2346 at 602.965 kb on + strand, within Ac3H11_2346 at 602.965 kb on + strand, within Ac3H11_2346 at 602.965 kb on + strand, within Ac3H11_2346 at 602.966 kb on - strand, within Ac3H11_2346 at 603.115 kb on + strand, within Ac3H11_2346 at 603.115 kb on + strand, within Ac3H11_2346 at 603.115 kb on + strand, within Ac3H11_2346 at 603.115 kb on + strand, within Ac3H11_2346 at 603.116 kb on - strand, within Ac3H11_2346 at 603.148 kb on + strand, within Ac3H11_2346 at 603.148 kb on + strand, within Ac3H11_2346 at 603.148 kb on + strand, within Ac3H11_2346 at 603.149 kb on - strand, within Ac3H11_2346 at 603.149 kb on - strand, within Ac3H11_2346 at 603.331 kb on + strand, within Ac3H11_2346 at 603.331 kb on + strand, within Ac3H11_2346 at 603.331 kb on + strand, within Ac3H11_2346 at 603.331 kb on + strand, within Ac3H11_2346 at 603.331 kb on + strand, within Ac3H11_2346 at 603.331 kb on + strand, within Ac3H11_2346 at 603.332 kb on - strand, within Ac3H11_2346 at 603.332 kb on - strand, within Ac3H11_2346 at 603.332 kb on - strand, within Ac3H11_2346 at 603.332 kb on - strand, within Ac3H11_2346 at 603.332 kb on - strand, within Ac3H11_2346
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH7 remove 599,236 - -1.1 599,236 - +0.5 599,300 + +0.2 599,300 + +0.4 599,300 + +1.0 599,300 + +0.2 599,301 - -0.9 599,301 - -0.1 599,301 - +0.1 599,304 + +0.1 599,304 + +0.3 599,305 - +0.3 599,305 - -0.2 599,378 - Ac3H11_2344 0.16 +0.8 599,378 - Ac3H11_2344 0.16 -0.5 599,388 + Ac3H11_2344 0.17 -1.1 599,388 + Ac3H11_2344 0.17 -0.0 599,403 + Ac3H11_2344 0.18 -1.3 599,562 + Ac3H11_2344 0.35 -0.1 599,562 + Ac3H11_2344 0.35 +1.6 599,563 - Ac3H11_2344 0.35 +0.3 599,673 + Ac3H11_2344 0.47 +0.1 599,673 + Ac3H11_2344 0.47 +0.2 599,673 + Ac3H11_2344 0.47 -0.2 599,673 + Ac3H11_2344 0.47 -0.0 600,292 - +2.8 600,516 + Ac3H11_2345 0.12 -0.6 600,516 + Ac3H11_2345 0.12 +0.0 600,517 - Ac3H11_2345 0.12 +0.5 600,517 - Ac3H11_2345 0.12 -0.7 600,593 + Ac3H11_2345 0.15 +0.7 600,725 + Ac3H11_2345 0.20 -0.0 600,725 + Ac3H11_2345 0.20 -0.4 600,726 - Ac3H11_2345 0.21 -0.6 600,726 - Ac3H11_2345 0.21 -0.2 600,731 + Ac3H11_2345 0.21 -0.2 600,731 + Ac3H11_2345 0.21 +0.9 600,731 + Ac3H11_2345 0.21 -0.6 600,732 - Ac3H11_2345 0.21 -0.9 600,929 + Ac3H11_2345 0.29 +0.4 601,002 + Ac3H11_2345 0.32 +0.4 601,088 + Ac3H11_2345 0.35 +0.8 601,088 + Ac3H11_2345 0.35 -0.7 601,088 + Ac3H11_2345 0.35 +0.5 601,089 - Ac3H11_2345 0.35 -0.0 601,089 - Ac3H11_2345 0.35 -0.4 601,263 + +1.9 601,264 - Ac3H11_2345 0.43 +0.1 601,267 + Ac3H11_2345 0.43 +0.5 601,267 + Ac3H11_2345 0.43 -0.7 601,267 + Ac3H11_2345 0.43 +2.2 601,267 + Ac3H11_2345 0.43 +1.1 601,267 + Ac3H11_2345 0.43 +0.2 601,267 + Ac3H11_2345 0.43 +0.2 601,268 - Ac3H11_2345 0.43 -0.2 601,268 - Ac3H11_2345 0.43 -2.1 601,268 - Ac3H11_2345 0.43 +0.3 601,268 - Ac3H11_2345 0.43 -0.9 601,745 + Ac3H11_2345 0.62 -0.3 601,745 + Ac3H11_2345 0.62 +0.3 601,745 + Ac3H11_2345 0.62 -0.3 601,745 + Ac3H11_2345 0.62 +0.0 601,746 - Ac3H11_2345 0.62 +0.5 601,746 - Ac3H11_2345 0.62 -0.2 601,746 - Ac3H11_2345 0.62 -0.0 601,746 - Ac3H11_2345 0.62 -0.3 601,746 - Ac3H11_2345 0.62 +0.1 601,746 - Ac3H11_2345 0.62 -0.1 601,746 - Ac3H11_2345 0.62 +0.5 601,837 + Ac3H11_2345 0.66 +0.4 601,854 - Ac3H11_2345 0.67 +0.1 601,854 - Ac3H11_2345 0.67 +0.6 601,886 - Ac3H11_2345 0.68 +0.4 601,910 + Ac3H11_2345 0.69 -0.6 601,910 + Ac3H11_2345 0.69 -0.6 601,910 + Ac3H11_2345 0.69 +0.0 601,911 - Ac3H11_2345 0.69 -0.7 601,911 - Ac3H11_2345 0.69 -1.2 601,911 - Ac3H11_2345 0.69 +0.3 601,911 - Ac3H11_2345 0.69 -0.4 601,944 - Ac3H11_2345 0.71 -1.0 601,991 + Ac3H11_2345 0.73 -0.4 601,991 + Ac3H11_2345 0.73 +0.0 602,225 + Ac3H11_2345 0.82 +0.0 602,225 + Ac3H11_2345 0.82 -0.6 602,225 + Ac3H11_2345 0.82 -0.2 602,226 - Ac3H11_2345 0.82 -1.8 602,226 - Ac3H11_2345 0.82 -0.2 602,307 - Ac3H11_2345 0.85 +0.9 602,307 - Ac3H11_2345 0.85 +0.2 602,307 - Ac3H11_2345 0.85 +0.3 602,447 + -1.4 602,447 + -0.2 602,447 + -0.2 602,690 + +1.0 602,774 - -0.1 602,774 - -1.0 602,791 - -0.1 602,803 + -0.9 602,803 + +0.6 602,804 - +0.3 602,903 - Ac3H11_2346 0.11 -0.4 602,903 - Ac3H11_2346 0.11 -0.1 602,965 + Ac3H11_2346 0.17 -1.0 602,965 + Ac3H11_2346 0.17 +0.1 602,965 + Ac3H11_2346 0.17 -0.2 602,965 + Ac3H11_2346 0.17 -0.8 602,966 - Ac3H11_2346 0.17 -0.3 603,115 + Ac3H11_2346 0.33 -0.7 603,115 + Ac3H11_2346 0.33 +0.7 603,115 + Ac3H11_2346 0.33 +0.6 603,115 + Ac3H11_2346 0.33 -0.7 603,116 - Ac3H11_2346 0.33 -0.1 603,148 + Ac3H11_2346 0.36 -0.2 603,148 + Ac3H11_2346 0.36 -0.1 603,148 + Ac3H11_2346 0.36 -0.5 603,149 - Ac3H11_2346 0.36 +0.8 603,149 - Ac3H11_2346 0.36 -1.3 603,331 + Ac3H11_2346 0.55 -0.4 603,331 + Ac3H11_2346 0.55 +0.0 603,331 + Ac3H11_2346 0.55 +0.1 603,331 + Ac3H11_2346 0.55 -0.2 603,331 + Ac3H11_2346 0.55 -0.9 603,331 + Ac3H11_2346 0.55 +0.9 603,332 - Ac3H11_2346 0.56 +0.4 603,332 - Ac3H11_2346 0.56 +0.7 603,332 - Ac3H11_2346 0.56 +0.5 603,332 - Ac3H11_2346 0.56 +0.3 603,332 - Ac3H11_2346 0.56 -0.9
Or see this region's nucleotide sequence