Experiment: Bacitracin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and rpsT are separated by 205 nucleotides rpsT and tatD are separated by 66 nucleotides tatD and rpoB are separated by 82 nucleotides
Synpcc7942_1519: hisD - histidinol dehydrogenase, at 1,570,976 to 1,572,280
hisD
Synpcc7942_1520: rpsT - 30S ribosomal protein S20, at 1,572,486 to 1,572,782
rpsT
Synpcc7942_1521: tatD - Sec-independent protein translocase TatD, at 1,572,849 to 1,573,646
tatD
Synpcc7942_1522: rpoB - DNA-directed RNA polymerase subunit beta, at 1,573,729 to 1,577,031
rpoB
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1572.359 kb on + strand at 1572.776 kb on + strand at 1572.776 kb on + strand at 1572.784 kb on - strand at 1572.784 kb on - strand at 1572.784 kb on - strand at 1572.784 kb on - strand at 1572.800 kb on + strand at 1572.800 kb on + strand at 1572.800 kb on + strand at 1572.806 kb on + strand at 1572.808 kb on + strand at 1572.808 kb on - strand at 1572.808 kb on - strand at 1572.808 kb on - strand at 1572.808 kb on - strand at 1572.808 kb on - strand at 1572.819 kb on + strand at 1572.839 kb on + strand at 1572.852 kb on - strand at 1572.944 kb on - strand, within tatD at 1572.951 kb on + strand, within tatD at 1572.951 kb on + strand, within tatD at 1572.959 kb on - strand, within tatD at 1572.959 kb on - strand, within tatD at 1572.959 kb on - strand, within tatD at 1572.987 kb on + strand, within tatD at 1572.995 kb on - strand, within tatD at 1573.042 kb on + strand, within tatD at 1573.043 kb on + strand, within tatD at 1573.055 kb on + strand, within tatD at 1573.056 kb on - strand, within tatD at 1573.056 kb on - strand, within tatD at 1573.104 kb on + strand, within tatD at 1573.156 kb on + strand, within tatD at 1573.158 kb on + strand, within tatD at 1573.166 kb on - strand, within tatD at 1573.190 kb on - strand, within tatD at 1573.190 kb on - strand, within tatD at 1573.226 kb on - strand, within tatD at 1573.245 kb on - strand, within tatD at 1573.281 kb on - strand, within tatD at 1573.289 kb on + strand, within tatD at 1573.291 kb on - strand, within tatD at 1573.309 kb on - strand, within tatD at 1573.314 kb on - strand, within tatD at 1573.314 kb on - strand, within tatD at 1573.395 kb on - strand, within tatD at 1573.403 kb on + strand, within tatD at 1573.413 kb on + strand, within tatD at 1573.496 kb on + strand, within tatD at 1573.551 kb on - strand, within tatD at 1573.563 kb on - strand, within tatD at 1573.564 kb on - strand, within tatD at 1573.572 kb on - strand at 1573.572 kb on - strand at 1573.600 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.0009765625 mg/ml remove 1,572,359 + +0.7 1,572,776 + -1.6 1,572,776 + -0.5 1,572,784 - +2.1 1,572,784 - -0.5 1,572,784 - -2.4 1,572,784 - -2.4 1,572,800 + -0.4 1,572,800 + -1.1 1,572,800 + +0.7 1,572,806 + +1.5 1,572,808 + -2.4 1,572,808 - -0.1 1,572,808 - -5.4 1,572,808 - -1.1 1,572,808 - -0.1 1,572,808 - -2.6 1,572,819 + +0.1 1,572,839 + +0.9 1,572,852 - -0.1 1,572,944 - tatD Synpcc7942_1521 0.12 -0.6 1,572,951 + tatD Synpcc7942_1521 0.13 -1.1 1,572,951 + tatD Synpcc7942_1521 0.13 -1.1 1,572,959 - tatD Synpcc7942_1521 0.14 +2.1 1,572,959 - tatD Synpcc7942_1521 0.14 -1.1 1,572,959 - tatD Synpcc7942_1521 0.14 -1.1 1,572,987 + tatD Synpcc7942_1521 0.17 -1.2 1,572,995 - tatD Synpcc7942_1521 0.18 -2.5 1,573,042 + tatD Synpcc7942_1521 0.24 -3.5 1,573,043 + tatD Synpcc7942_1521 0.24 -0.2 1,573,055 + tatD Synpcc7942_1521 0.26 -2.1 1,573,056 - tatD Synpcc7942_1521 0.26 -1.3 1,573,056 - tatD Synpcc7942_1521 0.26 -2.1 1,573,104 + tatD Synpcc7942_1521 0.32 -0.7 1,573,156 + tatD Synpcc7942_1521 0.38 -1.1 1,573,158 + tatD Synpcc7942_1521 0.39 -0.6 1,573,166 - tatD Synpcc7942_1521 0.40 -1.6 1,573,190 - tatD Synpcc7942_1521 0.43 -1.5 1,573,190 - tatD Synpcc7942_1521 0.43 -1.1 1,573,226 - tatD Synpcc7942_1521 0.47 -1.1 1,573,245 - tatD Synpcc7942_1521 0.50 +0.5 1,573,281 - tatD Synpcc7942_1521 0.54 -0.4 1,573,289 + tatD Synpcc7942_1521 0.55 +0.0 1,573,291 - tatD Synpcc7942_1521 0.55 +0.4 1,573,309 - tatD Synpcc7942_1521 0.58 -0.5 1,573,314 - tatD Synpcc7942_1521 0.58 -1.6 1,573,314 - tatD Synpcc7942_1521 0.58 -0.8 1,573,395 - tatD Synpcc7942_1521 0.68 -1.1 1,573,403 + tatD Synpcc7942_1521 0.69 +0.9 1,573,413 + tatD Synpcc7942_1521 0.71 -3.1 1,573,496 + tatD Synpcc7942_1521 0.81 -1.3 1,573,551 - tatD Synpcc7942_1521 0.88 -0.1 1,573,563 - tatD Synpcc7942_1521 0.89 -2.1 1,573,564 - tatD Synpcc7942_1521 0.90 -2.9 1,573,572 - -0.8 1,573,572 - -1.5 1,573,600 + -1.9
Or see this region's nucleotide sequence