Experiment: Bacitracin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0214 and Synpcc7942_0215 overlap by 9 nucleotides Synpcc7942_0215 and Synpcc7942_0216 are separated by 134 nucleotides
Synpcc7942_0214: Synpcc7942_0214 - hypothetical protein, at 216,509 to 217,432
_0214
Synpcc7942_0215: Synpcc7942_0215 - hypothetical protein, at 217,424 to 217,813
_0215
Synpcc7942_0216: Synpcc7942_0216 - putative zinc-binding oxidoreductase, at 217,948 to 218,964
_0216
Position (kb) 217
218 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 216.447 kb on + strand at 216.486 kb on + strand at 216.493 kb on - strand at 216.500 kb on - strand at 216.518 kb on + strand at 216.519 kb on + strand at 216.525 kb on - strand at 216.529 kb on + strand at 216.537 kb on - strand at 216.596 kb on - strand at 216.611 kb on - strand, within Synpcc7942_0214 at 216.618 kb on + strand, within Synpcc7942_0214 at 216.641 kb on + strand, within Synpcc7942_0214 at 216.642 kb on + strand, within Synpcc7942_0214 at 216.664 kb on - strand, within Synpcc7942_0214 at 216.673 kb on + strand, within Synpcc7942_0214 at 216.676 kb on + strand, within Synpcc7942_0214 at 216.678 kb on + strand, within Synpcc7942_0214 at 216.683 kb on + strand, within Synpcc7942_0214 at 216.683 kb on + strand, within Synpcc7942_0214 at 216.683 kb on + strand, within Synpcc7942_0214 at 216.683 kb on + strand, within Synpcc7942_0214 at 216.683 kb on + strand at 216.683 kb on + strand, within Synpcc7942_0214 at 216.683 kb on + strand, within Synpcc7942_0214 at 216.686 kb on - strand, within Synpcc7942_0214 at 216.688 kb on + strand, within Synpcc7942_0214 at 216.690 kb on + strand, within Synpcc7942_0214 at 216.690 kb on + strand, within Synpcc7942_0214 at 216.690 kb on + strand, within Synpcc7942_0214 at 216.690 kb on + strand, within Synpcc7942_0214 at 216.691 kb on - strand, within Synpcc7942_0214 at 216.691 kb on - strand, within Synpcc7942_0214 at 216.691 kb on - strand, within Synpcc7942_0214 at 216.691 kb on - strand, within Synpcc7942_0214 at 216.698 kb on + strand, within Synpcc7942_0214 at 216.698 kb on - strand, within Synpcc7942_0214 at 216.698 kb on - strand, within Synpcc7942_0214 at 216.698 kb on - strand, within Synpcc7942_0214 at 216.703 kb on - strand, within Synpcc7942_0214 at 216.706 kb on - strand, within Synpcc7942_0214 at 216.708 kb on + strand, within Synpcc7942_0214 at 216.721 kb on - strand, within Synpcc7942_0214 at 216.733 kb on + strand, within Synpcc7942_0214 at 216.765 kb on - strand, within Synpcc7942_0214 at 216.765 kb on - strand, within Synpcc7942_0214 at 216.789 kb on + strand, within Synpcc7942_0214 at 216.792 kb on + strand, within Synpcc7942_0214 at 216.800 kb on - strand, within Synpcc7942_0214 at 216.854 kb on - strand, within Synpcc7942_0214 at 216.861 kb on - strand, within Synpcc7942_0214 at 216.879 kb on + strand, within Synpcc7942_0214 at 216.882 kb on - strand, within Synpcc7942_0214 at 216.887 kb on - strand, within Synpcc7942_0214 at 216.889 kb on + strand, within Synpcc7942_0214 at 216.892 kb on - strand, within Synpcc7942_0214 at 216.897 kb on - strand, within Synpcc7942_0214 at 216.897 kb on - strand, within Synpcc7942_0214 at 216.897 kb on - strand, within Synpcc7942_0214 at 216.897 kb on - strand, within Synpcc7942_0214 at 216.904 kb on + strand, within Synpcc7942_0214 at 216.924 kb on + strand, within Synpcc7942_0214 at 216.932 kb on - strand, within Synpcc7942_0214 at 216.959 kb on + strand, within Synpcc7942_0214 at 216.969 kb on + strand, within Synpcc7942_0214 at 216.973 kb on + strand, within Synpcc7942_0214 at 216.981 kb on - strand, within Synpcc7942_0214 at 216.981 kb on - strand, within Synpcc7942_0214 at 217.035 kb on - strand, within Synpcc7942_0214 at 217.046 kb on - strand, within Synpcc7942_0214 at 217.048 kb on + strand, within Synpcc7942_0214 at 217.050 kb on + strand, within Synpcc7942_0214 at 217.058 kb on + strand, within Synpcc7942_0214 at 217.058 kb on - strand, within Synpcc7942_0214 at 217.102 kb on - strand, within Synpcc7942_0214 at 217.116 kb on + strand, within Synpcc7942_0214 at 217.116 kb on + strand, within Synpcc7942_0214 at 217.118 kb on + strand, within Synpcc7942_0214 at 217.148 kb on - strand, within Synpcc7942_0214 at 217.164 kb on + strand, within Synpcc7942_0214 at 217.164 kb on + strand, within Synpcc7942_0214 at 217.164 kb on + strand, within Synpcc7942_0214 at 217.172 kb on - strand, within Synpcc7942_0214 at 217.172 kb on - strand, within Synpcc7942_0214 at 217.172 kb on - strand, within Synpcc7942_0214 at 217.172 kb on - strand, within Synpcc7942_0214 at 217.172 kb on - strand, within Synpcc7942_0214 at 217.172 kb on - strand, within Synpcc7942_0214 at 217.206 kb on + strand, within Synpcc7942_0214 at 217.219 kb on + strand, within Synpcc7942_0214 at 217.223 kb on + strand, within Synpcc7942_0214 at 217.253 kb on + strand, within Synpcc7942_0214 at 217.300 kb on - strand, within Synpcc7942_0214 at 217.300 kb on - strand, within Synpcc7942_0214 at 217.320 kb on - strand, within Synpcc7942_0214 at 217.351 kb on + strand at 217.366 kb on + strand at 217.422 kb on - strand at 217.426 kb on + strand at 217.431 kb on + strand at 217.439 kb on - strand at 217.453 kb on - strand at 217.457 kb on - strand at 217.468 kb on - strand, within Synpcc7942_0215 at 217.515 kb on - strand, within Synpcc7942_0215 at 217.546 kb on - strand, within Synpcc7942_0215 at 217.552 kb on + strand, within Synpcc7942_0215 at 217.552 kb on + strand, within Synpcc7942_0215 at 217.571 kb on - strand, within Synpcc7942_0215 at 217.590 kb on + strand, within Synpcc7942_0215 at 217.595 kb on + strand, within Synpcc7942_0215 at 217.595 kb on - strand, within Synpcc7942_0215 at 217.600 kb on + strand, within Synpcc7942_0215 at 217.600 kb on + strand, within Synpcc7942_0215 at 217.600 kb on + strand, within Synpcc7942_0215 at 217.600 kb on + strand at 217.608 kb on - strand, within Synpcc7942_0215 at 217.608 kb on - strand, within Synpcc7942_0215 at 217.608 kb on - strand, within Synpcc7942_0215 at 217.608 kb on - strand, within Synpcc7942_0215 at 217.608 kb on - strand, within Synpcc7942_0215 at 217.635 kb on - strand, within Synpcc7942_0215 at 217.671 kb on - strand, within Synpcc7942_0215 at 217.675 kb on - strand, within Synpcc7942_0215 at 217.754 kb on + strand, within Synpcc7942_0215 at 217.754 kb on + strand, within Synpcc7942_0215 at 217.762 kb on - strand, within Synpcc7942_0215 at 217.797 kb on + strand at 217.805 kb on - strand at 217.805 kb on - strand at 217.817 kb on - strand at 217.825 kb on + strand at 217.834 kb on + strand at 217.839 kb on + strand at 217.841 kb on + strand at 217.842 kb on - strand at 217.849 kb on - strand at 218.010 kb on - strand at 218.010 kb on - strand at 218.038 kb on - strand at 218.043 kb on - strand at 218.062 kb on + strand, within Synpcc7942_0216 at 218.073 kb on + strand, within Synpcc7942_0216 at 218.118 kb on + strand, within Synpcc7942_0216 at 218.128 kb on + strand, within Synpcc7942_0216 at 218.134 kb on + strand, within Synpcc7942_0216 at 218.136 kb on - strand, within Synpcc7942_0216 at 218.172 kb on + strand, within Synpcc7942_0216 at 218.172 kb on - strand, within Synpcc7942_0216 at 218.172 kb on - strand, within Synpcc7942_0216 at 218.172 kb on - strand, within Synpcc7942_0216 at 218.190 kb on - strand at 218.230 kb on - strand, within Synpcc7942_0216 at 218.240 kb on - strand, within Synpcc7942_0216 at 218.271 kb on + strand, within Synpcc7942_0216 at 218.274 kb on - strand, within Synpcc7942_0216 at 218.286 kb on + strand, within Synpcc7942_0216 at 218.296 kb on - strand, within Synpcc7942_0216 at 218.296 kb on - strand, within Synpcc7942_0216 at 218.302 kb on + strand, within Synpcc7942_0216 at 218.309 kb on - strand, within Synpcc7942_0216 at 218.310 kb on - strand, within Synpcc7942_0216 at 218.319 kb on - strand, within Synpcc7942_0216 at 218.326 kb on + strand, within Synpcc7942_0216 at 218.329 kb on - strand, within Synpcc7942_0216 at 218.329 kb on - strand, within Synpcc7942_0216 at 218.334 kb on - strand, within Synpcc7942_0216 at 218.334 kb on - strand, within Synpcc7942_0216 at 218.381 kb on + strand, within Synpcc7942_0216 at 218.426 kb on + strand, within Synpcc7942_0216 at 218.433 kb on + strand, within Synpcc7942_0216 at 218.434 kb on - strand, within Synpcc7942_0216 at 218.472 kb on + strand, within Synpcc7942_0216 at 218.478 kb on - strand, within Synpcc7942_0216 at 218.497 kb on + strand, within Synpcc7942_0216 at 218.497 kb on + strand at 218.497 kb on + strand, within Synpcc7942_0216 at 218.497 kb on + strand, within Synpcc7942_0216 at 218.497 kb on + strand, within Synpcc7942_0216 at 218.497 kb on + strand, within Synpcc7942_0216 at 218.505 kb on - strand, within Synpcc7942_0216 at 218.505 kb on - strand, within Synpcc7942_0216 at 218.505 kb on - strand, within Synpcc7942_0216 at 218.505 kb on - strand, within Synpcc7942_0216 at 218.505 kb on - strand, within Synpcc7942_0216 at 218.505 kb on - strand, within Synpcc7942_0216 at 218.505 kb on - strand, within Synpcc7942_0216 at 218.516 kb on + strand, within Synpcc7942_0216 at 218.544 kb on + strand, within Synpcc7942_0216 at 218.552 kb on + strand, within Synpcc7942_0216 at 218.552 kb on + strand, within Synpcc7942_0216 at 218.557 kb on + strand, within Synpcc7942_0216 at 218.613 kb on + strand, within Synpcc7942_0216 at 218.614 kb on - strand, within Synpcc7942_0216 at 218.614 kb on - strand, within Synpcc7942_0216 at 218.618 kb on + strand, within Synpcc7942_0216 at 218.618 kb on + strand, within Synpcc7942_0216 at 218.626 kb on - strand, within Synpcc7942_0216 at 218.626 kb on - strand, within Synpcc7942_0216 at 218.631 kb on - strand, within Synpcc7942_0216 at 218.638 kb on + strand, within Synpcc7942_0216 at 218.656 kb on - strand, within Synpcc7942_0216 at 218.678 kb on - strand, within Synpcc7942_0216 at 218.686 kb on + strand, within Synpcc7942_0216 at 218.690 kb on - strand, within Synpcc7942_0216 at 218.690 kb on - strand, within Synpcc7942_0216 at 218.696 kb on + strand, within Synpcc7942_0216 at 218.702 kb on - strand, within Synpcc7942_0216 at 218.704 kb on - strand, within Synpcc7942_0216 at 218.716 kb on + strand, within Synpcc7942_0216 at 218.740 kb on + strand, within Synpcc7942_0216 at 218.740 kb on + strand, within Synpcc7942_0216 at 218.774 kb on - strand, within Synpcc7942_0216 at 218.811 kb on + strand, within Synpcc7942_0216 at 218.811 kb on + strand, within Synpcc7942_0216
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.0009765625 mg/ml remove 216,447 + +0.1 216,486 + -0.7 216,493 - +1.5 216,500 - -0.8 216,518 + +0.4 216,519 + +0.8 216,525 - +0.7 216,529 + -2.1 216,537 - +2.5 216,596 - +1.4 216,611 - Synpcc7942_0214 0.11 -1.1 216,618 + Synpcc7942_0214 0.12 +1.9 216,641 + Synpcc7942_0214 0.14 +1.9 216,642 + Synpcc7942_0214 0.14 +1.5 216,664 - Synpcc7942_0214 0.17 +0.8 216,673 + Synpcc7942_0214 0.18 +0.5 216,676 + Synpcc7942_0214 0.18 -0.7 216,678 + Synpcc7942_0214 0.18 -0.6 216,683 + Synpcc7942_0214 0.19 -0.2 216,683 + Synpcc7942_0214 0.19 +0.9 216,683 + Synpcc7942_0214 0.19 +1.5 216,683 + Synpcc7942_0214 0.19 +0.5 216,683 + -0.1 216,683 + Synpcc7942_0214 0.19 +0.7 216,683 + Synpcc7942_0214 0.19 -1.2 216,686 - Synpcc7942_0214 0.19 -1.2 216,688 + Synpcc7942_0214 0.19 +0.5 216,690 + Synpcc7942_0214 0.20 -0.7 216,690 + Synpcc7942_0214 0.20 -1.7 216,690 + Synpcc7942_0214 0.20 -2.1 216,690 + Synpcc7942_0214 0.20 -0.1 216,691 - Synpcc7942_0214 0.20 -1.1 216,691 - Synpcc7942_0214 0.20 +0.7 216,691 - Synpcc7942_0214 0.20 +1.1 216,691 - Synpcc7942_0214 0.20 +3.2 216,698 + Synpcc7942_0214 0.20 +0.3 216,698 - Synpcc7942_0214 0.20 +3.1 216,698 - Synpcc7942_0214 0.20 +0.1 216,698 - Synpcc7942_0214 0.20 +0.3 216,703 - Synpcc7942_0214 0.21 +0.9 216,706 - Synpcc7942_0214 0.21 +0.2 216,708 + Synpcc7942_0214 0.22 +0.9 216,721 - Synpcc7942_0214 0.23 +0.6 216,733 + Synpcc7942_0214 0.24 -0.2 216,765 - Synpcc7942_0214 0.28 +0.3 216,765 - Synpcc7942_0214 0.28 +0.1 216,789 + Synpcc7942_0214 0.30 -0.4 216,792 + Synpcc7942_0214 0.31 -3.9 216,800 - Synpcc7942_0214 0.31 -0.2 216,854 - Synpcc7942_0214 0.37 +0.9 216,861 - Synpcc7942_0214 0.38 +0.3 216,879 + Synpcc7942_0214 0.40 +0.6 216,882 - Synpcc7942_0214 0.40 +1.0 216,887 - Synpcc7942_0214 0.41 +0.7 216,889 + Synpcc7942_0214 0.41 -0.1 216,892 - Synpcc7942_0214 0.41 +0.3 216,897 - Synpcc7942_0214 0.42 +1.5 216,897 - Synpcc7942_0214 0.42 -0.6 216,897 - Synpcc7942_0214 0.42 +1.2 216,897 - Synpcc7942_0214 0.42 -2.1 216,904 + Synpcc7942_0214 0.43 +0.7 216,924 + Synpcc7942_0214 0.45 +0.5 216,932 - Synpcc7942_0214 0.46 +0.7 216,959 + Synpcc7942_0214 0.49 +0.3 216,969 + Synpcc7942_0214 0.50 -0.5 216,973 + Synpcc7942_0214 0.50 +1.2 216,981 - Synpcc7942_0214 0.51 -1.5 216,981 - Synpcc7942_0214 0.51 -0.7 217,035 - Synpcc7942_0214 0.57 -2.0 217,046 - Synpcc7942_0214 0.58 -0.3 217,048 + Synpcc7942_0214 0.58 +1.5 217,050 + Synpcc7942_0214 0.59 -0.7 217,058 + Synpcc7942_0214 0.59 +0.1 217,058 - Synpcc7942_0214 0.59 +1.4 217,102 - Synpcc7942_0214 0.64 +2.7 217,116 + Synpcc7942_0214 0.66 +2.3 217,116 + Synpcc7942_0214 0.66 -0.2 217,118 + Synpcc7942_0214 0.66 -1.1 217,148 - Synpcc7942_0214 0.69 -1.1 217,164 + Synpcc7942_0214 0.71 +1.9 217,164 + Synpcc7942_0214 0.71 +0.1 217,164 + Synpcc7942_0214 0.71 +3.2 217,172 - Synpcc7942_0214 0.72 -0.8 217,172 - Synpcc7942_0214 0.72 -1.0 217,172 - Synpcc7942_0214 0.72 -0.1 217,172 - Synpcc7942_0214 0.72 +0.4 217,172 - Synpcc7942_0214 0.72 +0.7 217,172 - Synpcc7942_0214 0.72 -1.4 217,206 + Synpcc7942_0214 0.75 +0.6 217,219 + Synpcc7942_0214 0.77 -1.3 217,223 + Synpcc7942_0214 0.77 +0.5 217,253 + Synpcc7942_0214 0.81 +1.1 217,300 - Synpcc7942_0214 0.86 +1.2 217,300 - Synpcc7942_0214 0.86 +0.4 217,320 - Synpcc7942_0214 0.88 +0.7 217,351 + -1.7 217,366 + +3.4 217,422 - +0.9 217,426 + -0.7 217,431 + -1.1 217,439 - +3.1 217,453 - -2.1 217,457 - -0.1 217,468 - Synpcc7942_0215 0.11 +0.5 217,515 - Synpcc7942_0215 0.23 +0.1 217,546 - Synpcc7942_0215 0.31 -1.1 217,552 + Synpcc7942_0215 0.33 -0.4 217,552 + Synpcc7942_0215 0.33 -1.1 217,571 - Synpcc7942_0215 0.38 +0.9 217,590 + Synpcc7942_0215 0.43 -4.2 217,595 + Synpcc7942_0215 0.44 -1.2 217,595 - Synpcc7942_0215 0.44 -1.7 217,600 + Synpcc7942_0215 0.45 -1.7 217,600 + Synpcc7942_0215 0.45 -0.2 217,600 + Synpcc7942_0215 0.45 -1.2 217,600 + -1.1 217,608 - Synpcc7942_0215 0.47 -0.9 217,608 - Synpcc7942_0215 0.47 -0.1 217,608 - Synpcc7942_0215 0.47 -1.7 217,608 - Synpcc7942_0215 0.47 -3.7 217,608 - Synpcc7942_0215 0.47 -0.7 217,635 - Synpcc7942_0215 0.54 -1.3 217,671 - Synpcc7942_0215 0.63 +2.5 217,675 - Synpcc7942_0215 0.64 +1.9 217,754 + Synpcc7942_0215 0.85 -3.1 217,754 + Synpcc7942_0215 0.85 -0.6 217,762 - Synpcc7942_0215 0.87 -0.4 217,797 + -0.3 217,805 - -1.7 217,805 - -1.7 217,817 - -0.1 217,825 + -2.9 217,834 + -0.1 217,839 + +0.2 217,841 + -0.4 217,842 - -1.1 217,849 - -1.7 218,010 - -1.7 218,010 - +0.2 218,038 - +0.5 218,043 - +1.4 218,062 + Synpcc7942_0216 0.11 +0.2 218,073 + Synpcc7942_0216 0.12 +0.3 218,118 + Synpcc7942_0216 0.17 -1.4 218,128 + Synpcc7942_0216 0.18 -0.2 218,134 + Synpcc7942_0216 0.18 +1.6 218,136 - Synpcc7942_0216 0.18 +0.7 218,172 + Synpcc7942_0216 0.22 +0.5 218,172 - Synpcc7942_0216 0.22 -0.7 218,172 - Synpcc7942_0216 0.22 -0.1 218,172 - Synpcc7942_0216 0.22 +0.4 218,190 - -1.1 218,230 - Synpcc7942_0216 0.28 -0.1 218,240 - Synpcc7942_0216 0.29 +0.0 218,271 + Synpcc7942_0216 0.32 -0.3 218,274 - Synpcc7942_0216 0.32 +0.3 218,286 + Synpcc7942_0216 0.33 -0.5 218,296 - Synpcc7942_0216 0.34 +1.7 218,296 - Synpcc7942_0216 0.34 +2.1 218,302 + Synpcc7942_0216 0.35 -0.4 218,309 - Synpcc7942_0216 0.35 +0.1 218,310 - Synpcc7942_0216 0.36 +1.2 218,319 - Synpcc7942_0216 0.36 +0.1 218,326 + Synpcc7942_0216 0.37 +0.7 218,329 - Synpcc7942_0216 0.37 -0.7 218,329 - Synpcc7942_0216 0.37 -1.9 218,334 - Synpcc7942_0216 0.38 -0.0 218,334 - Synpcc7942_0216 0.38 +4.2 218,381 + Synpcc7942_0216 0.43 -0.6 218,426 + Synpcc7942_0216 0.47 +0.3 218,433 + Synpcc7942_0216 0.48 +2.5 218,434 - Synpcc7942_0216 0.48 +0.7 218,472 + Synpcc7942_0216 0.52 +1.2 218,478 - Synpcc7942_0216 0.52 +0.9 218,497 + Synpcc7942_0216 0.54 +1.5 218,497 + +2.2 218,497 + Synpcc7942_0216 0.54 +1.2 218,497 + Synpcc7942_0216 0.54 -0.4 218,497 + Synpcc7942_0216 0.54 +1.2 218,497 + Synpcc7942_0216 0.54 -0.5 218,505 - Synpcc7942_0216 0.55 +0.5 218,505 - Synpcc7942_0216 0.55 +1.5 218,505 - Synpcc7942_0216 0.55 -1.1 218,505 - Synpcc7942_0216 0.55 -0.1 218,505 - Synpcc7942_0216 0.55 +0.1 218,505 - Synpcc7942_0216 0.55 +1.8 218,505 - Synpcc7942_0216 0.55 -2.5 218,516 + Synpcc7942_0216 0.56 +0.5 218,544 + Synpcc7942_0216 0.59 +0.2 218,552 + Synpcc7942_0216 0.59 +0.9 218,552 + Synpcc7942_0216 0.59 -2.7 218,557 + Synpcc7942_0216 0.60 +1.2 218,613 + Synpcc7942_0216 0.65 +0.5 218,614 - Synpcc7942_0216 0.65 -1.1 218,614 - Synpcc7942_0216 0.65 -1.7 218,618 + Synpcc7942_0216 0.66 +2.3 218,618 + Synpcc7942_0216 0.66 +2.1 218,626 - Synpcc7942_0216 0.67 +1.7 218,626 - Synpcc7942_0216 0.67 -0.2 218,631 - Synpcc7942_0216 0.67 -0.3 218,638 + Synpcc7942_0216 0.68 -1.7 218,656 - Synpcc7942_0216 0.70 +0.2 218,678 - Synpcc7942_0216 0.72 +0.7 218,686 + Synpcc7942_0216 0.73 +0.5 218,690 - Synpcc7942_0216 0.73 +1.0 218,690 - Synpcc7942_0216 0.73 +0.3 218,696 + Synpcc7942_0216 0.74 +1.9 218,702 - Synpcc7942_0216 0.74 +0.0 218,704 - Synpcc7942_0216 0.74 +0.6 218,716 + Synpcc7942_0216 0.76 -1.7 218,740 + Synpcc7942_0216 0.78 +0.3 218,740 + Synpcc7942_0216 0.78 +1.1 218,774 - Synpcc7942_0216 0.81 -1.1 218,811 + Synpcc7942_0216 0.85 +2.2 218,811 + Synpcc7942_0216 0.85 -0.1
Or see this region's nucleotide sequence