Experiment: Bacitracin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbC and rfbD overlap by 4 nucleotides rfbD and rfbB overlap by 4 nucleotides rfbB and rfbA overlap by 4 nucleotides rfbA and Synpcc7942_2102 are separated by 11 nucleotides
Synpcc7942_2098: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 2,180,457 to 2,181,008
rfbC
Synpcc7942_2099: rfbD - dTDP-4-dehydrorhamnose reductase, at 2,181,005 to 2,181,874
rfbD
Synpcc7942_2100: rfbB - dTDP-glucose 4,6-dehydratase, at 2,181,871 to 2,182,944
rfbB
Synpcc7942_2101: rfbA - glucose-1-phosphate thymidylyltransferase, at 2,182,941 to 2,183,825
rfbA
Synpcc7942_2102: Synpcc7942_2102 - NAD-reducing hydrogenase gamma, at 2,183,837 to 2,184,076
_2102
Position (kb)
2181
2182
2183 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2180.891 kb on + strand, within rfbC at 2180.899 kb on - strand, within rfbC at 2180.946 kb on + strand, within rfbC at 2180.954 kb on - strand at 2180.981 kb on + strand at 2181.019 kb on + strand at 2181.027 kb on + strand at 2181.037 kb on + strand at 2181.045 kb on - strand at 2181.174 kb on - strand, within rfbD at 2181.179 kb on + strand, within rfbD at 2181.207 kb on - strand, within rfbD at 2181.328 kb on + strand, within rfbD at 2181.342 kb on - strand, within rfbD at 2181.382 kb on + strand, within rfbD at 2181.449 kb on + strand, within rfbD at 2181.553 kb on + strand, within rfbD at 2181.561 kb on - strand, within rfbD at 2181.614 kb on + strand, within rfbD at 2181.622 kb on - strand, within rfbD at 2181.710 kb on + strand, within rfbD at 2181.718 kb on - strand, within rfbD at 2181.727 kb on + strand, within rfbD at 2181.763 kb on + strand, within rfbD at 2181.764 kb on + strand, within rfbD at 2181.773 kb on + strand, within rfbD at 2181.786 kb on - strand, within rfbD at 2181.815 kb on + strand at 2181.823 kb on - strand at 2181.890 kb on - strand at 2181.913 kb on + strand at 2181.921 kb on - strand at 2181.935 kb on - strand at 2182.006 kb on - strand, within rfbB at 2182.007 kb on + strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.038 kb on - strand, within rfbB at 2182.053 kb on + strand, within rfbB at 2182.093 kb on - strand, within rfbB at 2182.093 kb on - strand, within rfbB at 2182.107 kb on - strand, within rfbB at 2182.152 kb on - strand, within rfbB at 2182.202 kb on - strand, within rfbB at 2182.232 kb on + strand, within rfbB at 2182.258 kb on + strand, within rfbB at 2182.271 kb on - strand, within rfbB at 2182.278 kb on + strand, within rfbB at 2182.279 kb on + strand, within rfbB at 2182.286 kb on - strand, within rfbB at 2182.287 kb on - strand, within rfbB at 2182.362 kb on - strand, within rfbB at 2182.383 kb on + strand, within rfbB at 2182.395 kb on + strand, within rfbB at 2182.403 kb on - strand, within rfbB at 2182.405 kb on - strand, within rfbB at 2182.405 kb on - strand, within rfbB at 2182.457 kb on - strand, within rfbB at 2182.475 kb on - strand, within rfbB at 2182.489 kb on - strand, within rfbB at 2182.566 kb on - strand, within rfbB at 2182.571 kb on + strand, within rfbB at 2182.584 kb on - strand, within rfbB at 2182.584 kb on - strand, within rfbB at 2182.617 kb on - strand, within rfbB at 2182.641 kb on - strand, within rfbB at 2182.762 kb on + strand, within rfbB at 2182.921 kb on - strand at 2182.968 kb on - strand at 2182.991 kb on + strand at 2182.991 kb on + strand at 2182.991 kb on + strand at 2182.999 kb on - strand at 2183.003 kb on + strand at 2183.005 kb on + strand at 2183.005 kb on + strand at 2183.013 kb on - strand at 2183.013 kb on - strand at 2183.046 kb on - strand, within rfbA at 2183.063 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on - strand, within rfbA at 2183.115 kb on - strand, within rfbA at 2183.123 kb on - strand, within rfbA at 2183.123 kb on - strand, within rfbA at 2183.141 kb on + strand, within rfbA at 2183.177 kb on + strand, within rfbA at 2183.177 kb on + strand, within rfbA at 2183.207 kb on - strand, within rfbA at 2183.214 kb on - strand, within rfbA at 2183.238 kb on + strand, within rfbA at 2183.338 kb on + strand, within rfbA at 2183.383 kb on - strand, within rfbA at 2183.432 kb on + strand, within rfbA at 2183.432 kb on + strand, within rfbA at 2183.457 kb on + strand, within rfbA at 2183.466 kb on + strand, within rfbA at 2183.501 kb on + strand, within rfbA at 2183.513 kb on - strand, within rfbA at 2183.540 kb on - strand, within rfbA at 2183.549 kb on + strand, within rfbA at 2183.557 kb on - strand, within rfbA at 2183.574 kb on + strand, within rfbA at 2183.592 kb on - strand, within rfbA at 2183.609 kb on + strand, within rfbA at 2183.628 kb on + strand, within rfbA at 2183.628 kb on + strand, within rfbA at 2183.636 kb on - strand, within rfbA at 2183.636 kb on - strand, within rfbA at 2183.721 kb on + strand, within rfbA at 2183.740 kb on - strand at 2183.746 kb on + strand at 2183.754 kb on - strand at 2183.763 kb on + strand at 2183.771 kb on - strand at 2183.804 kb on - strand at 2183.813 kb on - strand at 2183.827 kb on - strand at 2183.841 kb on + strand at 2183.849 kb on + strand at 2183.849 kb on - strand at 2183.862 kb on + strand, within Synpcc7942_2102 at 2183.897 kb on + strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.924 kb on + strand, within Synpcc7942_2102 at 2183.932 kb on - strand, within Synpcc7942_2102
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.0009765625 mg/ml remove 2,180,891 + rfbC Synpcc7942_2098 0.79 +0.4 2,180,899 - rfbC Synpcc7942_2098 0.80 -2.2 2,180,946 + rfbC Synpcc7942_2098 0.89 +2.0 2,180,954 - +1.0 2,180,981 + -0.7 2,181,019 + +3.4 2,181,027 + +4.5 2,181,037 + +3.9 2,181,045 - +4.8 2,181,174 - rfbD Synpcc7942_2099 0.19 +4.8 2,181,179 + rfbD Synpcc7942_2099 0.20 +2.8 2,181,207 - rfbD Synpcc7942_2099 0.23 +0.9 2,181,328 + rfbD Synpcc7942_2099 0.37 +3.0 2,181,342 - rfbD Synpcc7942_2099 0.39 +3.9 2,181,382 + rfbD Synpcc7942_2099 0.43 +3.8 2,181,449 + rfbD Synpcc7942_2099 0.51 +4.1 2,181,553 + rfbD Synpcc7942_2099 0.63 +0.8 2,181,561 - rfbD Synpcc7942_2099 0.64 +3.6 2,181,614 + rfbD Synpcc7942_2099 0.70 +3.9 2,181,622 - rfbD Synpcc7942_2099 0.71 -1.2 2,181,710 + rfbD Synpcc7942_2099 0.81 +0.4 2,181,718 - rfbD Synpcc7942_2099 0.82 +1.1 2,181,727 + rfbD Synpcc7942_2099 0.83 +2.3 2,181,763 + rfbD Synpcc7942_2099 0.87 +4.0 2,181,764 + rfbD Synpcc7942_2099 0.87 +2.9 2,181,773 + rfbD Synpcc7942_2099 0.88 +4.3 2,181,786 - rfbD Synpcc7942_2099 0.90 +2.2 2,181,815 + -0.2 2,181,823 - +1.8 2,181,890 - -4.0 2,181,913 + -0.7 2,181,921 - +3.3 2,181,935 - +0.3 2,182,006 - rfbB Synpcc7942_2100 0.13 +0.2 2,182,007 + rfbB Synpcc7942_2100 0.13 -0.2 2,182,015 - rfbB Synpcc7942_2100 0.13 +0.3 2,182,015 - rfbB Synpcc7942_2100 0.13 -2.5 2,182,015 - rfbB Synpcc7942_2100 0.13 +0.1 2,182,038 - rfbB Synpcc7942_2100 0.16 +1.3 2,182,053 + rfbB Synpcc7942_2100 0.17 +1.1 2,182,093 - rfbB Synpcc7942_2100 0.21 +0.2 2,182,093 - rfbB Synpcc7942_2100 0.21 +1.1 2,182,107 - rfbB Synpcc7942_2100 0.22 +0.3 2,182,152 - rfbB Synpcc7942_2100 0.26 +1.6 2,182,202 - rfbB Synpcc7942_2100 0.31 +0.7 2,182,232 + rfbB Synpcc7942_2100 0.34 +0.2 2,182,258 + rfbB Synpcc7942_2100 0.36 -0.9 2,182,271 - rfbB Synpcc7942_2100 0.37 -1.7 2,182,278 + rfbB Synpcc7942_2100 0.38 +3.5 2,182,279 + rfbB Synpcc7942_2100 0.38 +0.3 2,182,286 - rfbB Synpcc7942_2100 0.39 +0.2 2,182,287 - rfbB Synpcc7942_2100 0.39 +1.5 2,182,362 - rfbB Synpcc7942_2100 0.46 -2.0 2,182,383 + rfbB Synpcc7942_2100 0.48 +0.3 2,182,395 + rfbB Synpcc7942_2100 0.49 +1.1 2,182,403 - rfbB Synpcc7942_2100 0.50 -0.7 2,182,405 - rfbB Synpcc7942_2100 0.50 -0.2 2,182,405 - rfbB Synpcc7942_2100 0.50 +0.0 2,182,457 - rfbB Synpcc7942_2100 0.55 -0.2 2,182,475 - rfbB Synpcc7942_2100 0.56 -1.4 2,182,489 - rfbB Synpcc7942_2100 0.58 -1.2 2,182,566 - rfbB Synpcc7942_2100 0.65 +1.5 2,182,571 + rfbB Synpcc7942_2100 0.65 -1.2 2,182,584 - rfbB Synpcc7942_2100 0.66 +1.3 2,182,584 - rfbB Synpcc7942_2100 0.66 +0.6 2,182,617 - rfbB Synpcc7942_2100 0.69 +2.5 2,182,641 - rfbB Synpcc7942_2100 0.72 -0.6 2,182,762 + rfbB Synpcc7942_2100 0.83 +0.8 2,182,921 - -1.7 2,182,968 - -2.7 2,182,991 + -2.4 2,182,991 + +1.6 2,182,991 + -0.4 2,182,999 - -1.7 2,183,003 + +0.5 2,183,005 + -2.7 2,183,005 + -1.0 2,183,013 - +2.3 2,183,013 - +0.5 2,183,046 - rfbA Synpcc7942_2101 0.12 -1.7 2,183,063 + rfbA Synpcc7942_2101 0.14 -0.8 2,183,115 + rfbA Synpcc7942_2101 0.20 +1.0 2,183,115 + rfbA Synpcc7942_2101 0.20 -2.0 2,183,115 + rfbA Synpcc7942_2101 0.20 +2.7 2,183,115 - rfbA Synpcc7942_2101 0.20 +0.3 2,183,115 - rfbA Synpcc7942_2101 0.20 +2.2 2,183,123 - rfbA Synpcc7942_2101 0.21 +3.3 2,183,123 - rfbA Synpcc7942_2101 0.21 +0.8 2,183,141 + rfbA Synpcc7942_2101 0.23 -1.7 2,183,177 + rfbA Synpcc7942_2101 0.27 +3.0 2,183,177 + rfbA Synpcc7942_2101 0.27 -1.4 2,183,207 - rfbA Synpcc7942_2101 0.30 +0.3 2,183,214 - rfbA Synpcc7942_2101 0.31 +1.7 2,183,238 + rfbA Synpcc7942_2101 0.34 +2.2 2,183,338 + rfbA Synpcc7942_2101 0.45 -1.2 2,183,383 - rfbA Synpcc7942_2101 0.50 +0.3 2,183,432 + rfbA Synpcc7942_2101 0.55 -2.8 2,183,432 + rfbA Synpcc7942_2101 0.55 +0.9 2,183,457 + rfbA Synpcc7942_2101 0.58 -1.2 2,183,466 + rfbA Synpcc7942_2101 0.59 -2.8 2,183,501 + rfbA Synpcc7942_2101 0.63 +1.6 2,183,513 - rfbA Synpcc7942_2101 0.65 +0.3 2,183,540 - rfbA Synpcc7942_2101 0.68 +0.1 2,183,549 + rfbA Synpcc7942_2101 0.69 +2.0 2,183,557 - rfbA Synpcc7942_2101 0.70 +0.3 2,183,574 + rfbA Synpcc7942_2101 0.72 -0.7 2,183,592 - rfbA Synpcc7942_2101 0.74 +0.1 2,183,609 + rfbA Synpcc7942_2101 0.75 -0.7 2,183,628 + rfbA Synpcc7942_2101 0.78 -2.5 2,183,628 + rfbA Synpcc7942_2101 0.78 +2.7 2,183,636 - rfbA Synpcc7942_2101 0.79 +0.8 2,183,636 - rfbA Synpcc7942_2101 0.79 -2.0 2,183,721 + rfbA Synpcc7942_2101 0.88 -0.7 2,183,740 - -2.8 2,183,746 + -3.5 2,183,754 - +1.3 2,183,763 + -2.5 2,183,771 - -0.2 2,183,804 - +0.6 2,183,813 - +0.4 2,183,827 - +1.1 2,183,841 + +1.8 2,183,849 + +1.3 2,183,849 - -2.0 2,183,862 + Synpcc7942_2102 0.10 +3.0 2,183,897 + Synpcc7942_2102 0.25 +2.3 2,183,905 - Synpcc7942_2102 0.28 +2.6 2,183,905 - Synpcc7942_2102 0.28 +2.1 2,183,905 - Synpcc7942_2102 0.28 +1.9 2,183,905 - Synpcc7942_2102 0.28 +3.5 2,183,924 + Synpcc7942_2102 0.36 +1.6 2,183,932 - Synpcc7942_2102 0.40 +1.4
Or see this region's nucleotide sequence