Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2210 and Ac3H11_2211 overlap by 9 nucleotides Ac3H11_2211 and Ac3H11_2212 overlap by 44 nucleotides Ac3H11_2212 and Ac3H11_2213 overlap by 4 nucleotides
Ac3H11_2210 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 440,264 to 444,100
_2210
Ac3H11_2211 - hypothetical protein, at 444,092 to 444,304
_2211
Ac3H11_2212 - Permease of the drug/metabolite transporter (DMT) superfamily, at 444,261 to 445,187
_2212
Ac3H11_2213 - Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 445,184 to 445,756
_2213
Position (kb)
444
445 Strain fitness (log2 ratio)
-2
-1
0
1 at 443.256 kb on + strand, within Ac3H11_2210 at 443.256 kb on + strand, within Ac3H11_2210 at 443.256 kb on + strand, within Ac3H11_2210 at 443.256 kb on + strand, within Ac3H11_2210 at 443.256 kb on + strand, within Ac3H11_2210 at 443.256 kb on + strand, within Ac3H11_2210 at 443.257 kb on - strand, within Ac3H11_2210 at 443.257 kb on - strand, within Ac3H11_2210 at 443.334 kb on + strand, within Ac3H11_2210 at 443.386 kb on - strand, within Ac3H11_2210 at 443.394 kb on + strand, within Ac3H11_2210 at 443.394 kb on + strand, within Ac3H11_2210 at 443.395 kb on - strand, within Ac3H11_2210 at 443.395 kb on - strand, within Ac3H11_2210 at 443.395 kb on - strand, within Ac3H11_2210 at 443.877 kb on + strand at 443.877 kb on + strand at 443.877 kb on + strand at 443.877 kb on + strand at 443.878 kb on - strand at 444.224 kb on - strand, within Ac3H11_2211 at 444.224 kb on - strand, within Ac3H11_2211 at 444.305 kb on + strand at 444.305 kb on + strand at 444.305 kb on + strand at 444.306 kb on - strand at 444.339 kb on + strand at 444.339 kb on + strand at 444.340 kb on - strand at 444.340 kb on - strand at 444.397 kb on + strand, within Ac3H11_2212 at 444.398 kb on - strand, within Ac3H11_2212 at 444.398 kb on - strand, within Ac3H11_2212 at 444.398 kb on - strand, within Ac3H11_2212 at 444.476 kb on - strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.484 kb on + strand, within Ac3H11_2212 at 444.485 kb on - strand, within Ac3H11_2212 at 444.485 kb on - strand, within Ac3H11_2212 at 444.485 kb on - strand, within Ac3H11_2212 at 444.485 kb on - strand, within Ac3H11_2212 at 444.485 kb on - strand, within Ac3H11_2212 at 444.485 kb on - strand, within Ac3H11_2212 at 444.567 kb on + strand, within Ac3H11_2212 at 444.567 kb on + strand, within Ac3H11_2212 at 444.567 kb on + strand, within Ac3H11_2212 at 444.567 kb on + strand, within Ac3H11_2212 at 444.568 kb on - strand, within Ac3H11_2212 at 444.568 kb on - strand, within Ac3H11_2212 at 444.676 kb on + strand, within Ac3H11_2212 at 444.677 kb on - strand, within Ac3H11_2212 at 444.677 kb on - strand, within Ac3H11_2212 at 444.677 kb on - strand, within Ac3H11_2212 at 444.677 kb on - strand, within Ac3H11_2212 at 444.677 kb on - strand, within Ac3H11_2212 at 444.677 kb on - strand, within Ac3H11_2212 at 444.677 kb on - strand, within Ac3H11_2212 at 444.997 kb on + strand, within Ac3H11_2212 at 445.303 kb on - strand, within Ac3H11_2213
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 443,256 + Ac3H11_2210 0.78 +0.2 443,256 + Ac3H11_2210 0.78 -0.0 443,256 + Ac3H11_2210 0.78 +0.5 443,256 + Ac3H11_2210 0.78 +0.3 443,256 + Ac3H11_2210 0.78 +0.2 443,256 + Ac3H11_2210 0.78 +0.4 443,257 - Ac3H11_2210 0.78 +0.1 443,257 - Ac3H11_2210 0.78 +0.3 443,334 + Ac3H11_2210 0.80 -1.0 443,386 - Ac3H11_2210 0.81 +0.0 443,394 + Ac3H11_2210 0.82 -1.0 443,394 + Ac3H11_2210 0.82 -0.2 443,395 - Ac3H11_2210 0.82 +0.6 443,395 - Ac3H11_2210 0.82 +0.4 443,395 - Ac3H11_2210 0.82 -0.2 443,877 + -0.5 443,877 + +0.1 443,877 + +0.6 443,877 + -0.7 443,878 - -0.5 444,224 - Ac3H11_2211 0.62 +0.1 444,224 - Ac3H11_2211 0.62 +0.6 444,305 + +0.6 444,305 + +0.1 444,305 + +1.0 444,306 - -0.0 444,339 + +0.2 444,339 + +0.1 444,340 - +0.1 444,340 - +1.2 444,397 + Ac3H11_2212 0.15 -0.4 444,398 - Ac3H11_2212 0.15 -0.4 444,398 - Ac3H11_2212 0.15 -0.7 444,398 - Ac3H11_2212 0.15 +0.2 444,476 - Ac3H11_2212 0.23 -0.5 444,484 + Ac3H11_2212 0.24 +0.2 444,484 + Ac3H11_2212 0.24 +0.0 444,484 + Ac3H11_2212 0.24 +0.1 444,484 + Ac3H11_2212 0.24 +0.5 444,484 + Ac3H11_2212 0.24 +0.6 444,484 + Ac3H11_2212 0.24 +0.2 444,484 + Ac3H11_2212 0.24 +1.5 444,484 + Ac3H11_2212 0.24 +0.1 444,484 + Ac3H11_2212 0.24 +0.6 444,485 - Ac3H11_2212 0.24 +0.1 444,485 - Ac3H11_2212 0.24 -0.4 444,485 - Ac3H11_2212 0.24 -0.0 444,485 - Ac3H11_2212 0.24 -0.4 444,485 - Ac3H11_2212 0.24 -0.7 444,485 - Ac3H11_2212 0.24 -2.4 444,567 + Ac3H11_2212 0.33 +1.6 444,567 + Ac3H11_2212 0.33 -0.9 444,567 + Ac3H11_2212 0.33 -0.2 444,567 + Ac3H11_2212 0.33 -0.1 444,568 - Ac3H11_2212 0.33 -1.0 444,568 - Ac3H11_2212 0.33 +0.4 444,676 + Ac3H11_2212 0.45 -0.1 444,677 - Ac3H11_2212 0.45 +0.5 444,677 - Ac3H11_2212 0.45 +0.6 444,677 - Ac3H11_2212 0.45 -0.2 444,677 - Ac3H11_2212 0.45 -0.0 444,677 - Ac3H11_2212 0.45 -0.7 444,677 - Ac3H11_2212 0.45 +0.2 444,677 - Ac3H11_2212 0.45 -0.2 444,997 + Ac3H11_2212 0.79 -0.5 445,303 - Ac3H11_2213 0.21 +0.3
Or see this region's nucleotide sequence