Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21365 and HSERO_RS21370 are separated by 164 nucleotides HSERO_RS21370 and HSERO_RS21375 overlap by 1 nucleotides HSERO_RS21375 and HSERO_RS21380 are separated by 14 nucleotides
HSERO_RS21365: HSERO_RS21365 - peptide chain release factor 1, at 4,890,053 to 4,891,120
_RS21365
HSERO_RS21370: HSERO_RS21370 - dihydrolipoamide acyltransferase, at 4,891,285 to 4,891,779
_RS21370
HSERO_RS21375: HSERO_RS21375 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 4,891,779 to 4,892,612
_RS21375
HSERO_RS21380: HSERO_RS21380 - MFS transporter, at 4,892,627 to 4,896,226
_RS21380
Position (kb) 4891
4892 Strain fitness (log2 ratio) -2
-1
0
1 at 4891.217 kb on + strand at 4891.331 kb on - strand at 4891.331 kb on - strand at 4891.483 kb on + strand, within HSERO_RS21370 at 4891.568 kb on - strand, within HSERO_RS21370 at 4891.823 kb on + strand at 4891.823 kb on + strand at 4891.996 kb on - strand, within HSERO_RS21375 at 4892.342 kb on - strand, within HSERO_RS21375 at 4892.497 kb on + strand, within HSERO_RS21375 at 4892.553 kb on + strand at 4892.588 kb on + strand at 4892.615 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 4,891,217 + +1.2 4,891,331 - -2.6 4,891,331 - -0.3 4,891,483 + HSERO_RS21370 0.40 -1.3 4,891,568 - HSERO_RS21370 0.57 -2.4 4,891,823 + -0.7 4,891,823 + -0.0 4,891,996 - HSERO_RS21375 0.26 -0.4 4,892,342 - HSERO_RS21375 0.68 -0.1 4,892,497 + HSERO_RS21375 0.86 -1.9 4,892,553 + +1.7 4,892,588 + -0.1 4,892,615 + -0.2
Or see this region's nucleotide sequence