Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19190 and HSERO_RS19195 are separated by 117 nucleotides HSERO_RS19195 and HSERO_RS19200 are separated by 71 nucleotides HSERO_RS19200 and HSERO_RS19205 are separated by 9 nucleotides HSERO_RS19205 and HSERO_RS19210 are separated by 23 nucleotides
HSERO_RS19190: HSERO_RS19190 - glutamate-1-semialdehyde 2,1-aminomutase, at 4,403,602 to 4,404,885
_RS19190
HSERO_RS19195: HSERO_RS19195 - iron--sulfur cluster insertion protein ErpA, at 4,405,003 to 4,405,368
_RS19195
HSERO_RS19200: HSERO_RS19200 - cell shape determination protein CcmA, at 4,405,440 to 4,405,835
_RS19200
HSERO_RS19205: HSERO_RS19205 - hypothetical protein, at 4,405,845 to 4,406,555
_RS19205
HSERO_RS19210: HSERO_RS19210 - N-acetyl-gamma-glutamyl-phosphate reductase, at 4,406,579 to 4,407,616
_RS19210
Position (kb)
4405
4406
4407 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4404.922 kb on - strand at 4405.428 kb on - strand at 4405.486 kb on + strand, within HSERO_RS19200 at 4405.490 kb on + strand, within HSERO_RS19200 at 4405.498 kb on - strand, within HSERO_RS19200 at 4405.498 kb on - strand, within HSERO_RS19200 at 4405.545 kb on + strand, within HSERO_RS19200 at 4405.612 kb on - strand at 4405.862 kb on + strand at 4405.899 kb on - strand at 4405.909 kb on - strand at 4405.941 kb on + strand, within HSERO_RS19205 at 4406.146 kb on + strand, within HSERO_RS19205 at 4406.274 kb on + strand, within HSERO_RS19205 at 4406.446 kb on - strand, within HSERO_RS19205 at 4406.493 kb on - strand at 4406.777 kb on - strand, within HSERO_RS19210 at 4407.057 kb on + strand, within HSERO_RS19210 at 4407.058 kb on - strand, within HSERO_RS19210 at 4407.060 kb on - strand, within HSERO_RS19210 at 4407.126 kb on + strand, within HSERO_RS19210 at 4407.134 kb on - strand, within HSERO_RS19210 at 4407.251 kb on - strand, within HSERO_RS19210
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 4,404,922 - +0.1 4,405,428 - +1.0 4,405,486 + HSERO_RS19200 0.12 -0.3 4,405,490 + HSERO_RS19200 0.13 +0.4 4,405,498 - HSERO_RS19200 0.15 -1.1 4,405,498 - HSERO_RS19200 0.15 +0.4 4,405,545 + HSERO_RS19200 0.27 -0.7 4,405,612 - -2.1 4,405,862 + -1.4 4,405,899 - -0.4 4,405,909 - +0.1 4,405,941 + HSERO_RS19205 0.14 -0.1 4,406,146 + HSERO_RS19205 0.42 -1.2 4,406,274 + HSERO_RS19205 0.60 -0.6 4,406,446 - HSERO_RS19205 0.85 -1.3 4,406,493 - +0.1 4,406,777 - HSERO_RS19210 0.19 +0.5 4,407,057 + HSERO_RS19210 0.46 -2.8 4,407,058 - HSERO_RS19210 0.46 -0.1 4,407,060 - HSERO_RS19210 0.46 +2.1 4,407,126 + HSERO_RS19210 0.53 -1.1 4,407,134 - HSERO_RS19210 0.53 +0.6 4,407,251 - HSERO_RS19210 0.65 -0.3
Or see this region's nucleotide sequence