Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS15550 and HSERO_RS15555 overlap by 8 nucleotides HSERO_RS15555 and HSERO_RS15560 are separated by 156 nucleotides HSERO_RS15560 and HSERO_RS15565 are separated by 49 nucleotides
HSERO_RS15550: HSERO_RS15550 - sulfite reductase, at 3,554,001 to 3,555,698
_RS15550
HSERO_RS15555: HSERO_RS15555 - oxidoreductase, at 3,555,691 to 3,556,218
_RS15555
HSERO_RS15560: HSERO_RS15560 - phosphoadenosine phosphosulfate reductase, at 3,556,375 to 3,557,106
_RS15560
HSERO_RS15565: HSERO_RS15565 - sulfate adenylyltransferase subunit 2, at 3,557,156 to 3,558,091
_RS15565
Position (kb)
3555
3556
3557 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3554.937 kb on + strand, within HSERO_RS15550 at 3555.198 kb on + strand, within HSERO_RS15550 at 3555.739 kb on + strand at 3555.835 kb on + strand, within HSERO_RS15555 at 3555.991 kb on + strand, within HSERO_RS15555 at 3556.004 kb on - strand, within HSERO_RS15555 at 3556.033 kb on - strand, within HSERO_RS15555 at 3556.279 kb on + strand at 3556.279 kb on + strand at 3556.326 kb on + strand at 3556.334 kb on - strand at 3556.753 kb on + strand, within HSERO_RS15560 at 3556.885 kb on - strand, within HSERO_RS15560 at 3556.905 kb on - strand, within HSERO_RS15560
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 3,554,937 + HSERO_RS15550 0.55 -3.9 3,555,198 + HSERO_RS15550 0.70 -4.1 3,555,739 + -4.5 3,555,835 + HSERO_RS15555 0.27 -2.7 3,555,991 + HSERO_RS15555 0.57 -4.8 3,556,004 - HSERO_RS15555 0.59 -3.1 3,556,033 - HSERO_RS15555 0.65 -4.7 3,556,279 + -0.0 3,556,279 + -0.3 3,556,326 + +0.1 3,556,334 - -3.7 3,556,753 + HSERO_RS15560 0.52 -2.7 3,556,885 - HSERO_RS15560 0.70 -4.4 3,556,905 - HSERO_RS15560 0.72 -1.4
Or see this region's nucleotide sequence