Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14350 and HSERO_RS14355 are separated by 11 nucleotides HSERO_RS14355 and HSERO_RS14360 overlap by 4 nucleotides HSERO_RS14360 and HSERO_RS14365 are separated by 17 nucleotides HSERO_RS14365 and HSERO_RS14370 are separated by 17 nucleotides
HSERO_RS14350: HSERO_RS14350 - cysteine desulfurase, at 3,252,485 to 3,253,624
_RS14350
HSERO_RS14355: HSERO_RS14355 - hypothetical protein, at 3,253,636 to 3,254,010
_RS14355
HSERO_RS14360: HSERO_RS14360 - iron-sulfur cofactor synthesis protein, at 3,254,007 to 3,254,255
_RS14360
HSERO_RS14365: HSERO_RS14365 - nitrogen fixation protein NifZ, at 3,254,273 to 3,254,569
_RS14365
HSERO_RS14370: HSERO_RS14370 - hypothetical protein, at 3,254,587 to 3,255,345
_RS14370
Position (kb)
3253
3254
3255 Strain fitness (log2 ratio)
-1
0
1 at 3252.728 kb on - strand, within HSERO_RS14350 at 3252.834 kb on - strand, within HSERO_RS14350 at 3252.834 kb on - strand, within HSERO_RS14350 at 3252.869 kb on + strand, within HSERO_RS14350 at 3253.003 kb on + strand, within HSERO_RS14350 at 3253.080 kb on - strand, within HSERO_RS14350 at 3253.284 kb on + strand, within HSERO_RS14350 at 3253.330 kb on + strand, within HSERO_RS14350 at 3253.476 kb on + strand, within HSERO_RS14350 at 3253.543 kb on - strand at 3253.614 kb on + strand at 3253.650 kb on + strand at 3253.677 kb on + strand, within HSERO_RS14355 at 3253.684 kb on + strand, within HSERO_RS14355 at 3253.845 kb on - strand, within HSERO_RS14355 at 3253.845 kb on - strand, within HSERO_RS14355 at 3253.975 kb on + strand at 3254.501 kb on - strand, within HSERO_RS14365 at 3254.668 kb on + strand, within HSERO_RS14370 at 3254.764 kb on - strand, within HSERO_RS14370 at 3254.846 kb on + strand, within HSERO_RS14370
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 3,252,728 - HSERO_RS14350 0.21 -0.4 3,252,834 - HSERO_RS14350 0.31 +1.2 3,252,834 - HSERO_RS14350 0.31 +1.5 3,252,869 + HSERO_RS14350 0.34 +0.2 3,253,003 + HSERO_RS14350 0.45 +0.2 3,253,080 - HSERO_RS14350 0.52 -0.2 3,253,284 + HSERO_RS14350 0.70 -0.1 3,253,330 + HSERO_RS14350 0.74 +0.5 3,253,476 + HSERO_RS14350 0.87 +0.0 3,253,543 - -0.5 3,253,614 + -1.1 3,253,650 + +0.1 3,253,677 + HSERO_RS14355 0.11 -0.4 3,253,684 + HSERO_RS14355 0.13 +0.6 3,253,845 - HSERO_RS14355 0.56 -0.3 3,253,845 - HSERO_RS14355 0.56 +0.4 3,253,975 + -0.1 3,254,501 - HSERO_RS14365 0.77 -0.3 3,254,668 + HSERO_RS14370 0.11 -0.1 3,254,764 - HSERO_RS14370 0.23 -0.7 3,254,846 + HSERO_RS14370 0.34 +0.3
Or see this region's nucleotide sequence