Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13295 and HSERO_RS13300 overlap by 4 nucleotides HSERO_RS13300 and HSERO_RS13305 are separated by 11 nucleotides HSERO_RS13305 and HSERO_RS13310 overlap by 4 nucleotides
HSERO_RS13295: HSERO_RS13295 - membrane protein, at 3,028,691 to 3,030,268
_RS13295
HSERO_RS13300: HSERO_RS13300 - cobalt-precorrin-6Y C(5)-methyltransferase, at 3,030,265 to 3,030,990
_RS13300
HSERO_RS13305: HSERO_RS13305 - magnesium chelatase, at 3,031,002 to 3,032,993
_RS13305
HSERO_RS13310: HSERO_RS13310 - cobaltochelatase, at 3,032,990 to 3,037,183
_RS13310
Position (kb)
3031
3032
3033 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3030.047 kb on - strand, within HSERO_RS13295 at 3030.324 kb on + strand at 3030.340 kb on - strand, within HSERO_RS13300 at 3030.444 kb on + strand, within HSERO_RS13300 at 3031.038 kb on - strand at 3031.678 kb on - strand, within HSERO_RS13305 at 3031.731 kb on - strand, within HSERO_RS13305 at 3032.443 kb on - strand, within HSERO_RS13305 at 3032.513 kb on + strand, within HSERO_RS13305 at 3032.651 kb on + strand, within HSERO_RS13305 at 3032.788 kb on + strand, within HSERO_RS13305 at 3032.845 kb on - strand at 3032.930 kb on - strand at 3033.051 kb on + strand at 3033.309 kb on - strand at 3033.505 kb on - strand, within HSERO_RS13310 at 3033.768 kb on + strand, within HSERO_RS13310 at 3033.834 kb on + strand, within HSERO_RS13310 at 3033.844 kb on - strand, within HSERO_RS13310 at 3033.984 kb on + strand, within HSERO_RS13310
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 3,030,047 - HSERO_RS13295 0.86 -0.3 3,030,324 + -1.3 3,030,340 - HSERO_RS13300 0.10 -0.4 3,030,444 + HSERO_RS13300 0.25 +1.3 3,031,038 - +0.3 3,031,678 - HSERO_RS13305 0.34 -2.9 3,031,731 - HSERO_RS13305 0.37 -0.9 3,032,443 - HSERO_RS13305 0.72 -0.6 3,032,513 + HSERO_RS13305 0.76 -1.2 3,032,651 + HSERO_RS13305 0.83 -0.7 3,032,788 + HSERO_RS13305 0.90 -0.8 3,032,845 - +0.2 3,032,930 - -0.2 3,033,051 + -0.9 3,033,309 - -0.3 3,033,505 - HSERO_RS13310 0.12 -1.4 3,033,768 + HSERO_RS13310 0.19 -0.0 3,033,834 + HSERO_RS13310 0.20 -1.0 3,033,844 - HSERO_RS13310 0.20 -0.3 3,033,984 + HSERO_RS13310 0.24 -0.5
Or see this region's nucleotide sequence