Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10640 and HSERO_RS10645 are separated by 59 nucleotides HSERO_RS10645 and HSERO_RS10650 are separated by 85 nucleotides HSERO_RS10650 and HSERO_RS10655 are separated by 339 nucleotides
HSERO_RS10640: HSERO_RS10640 - DNA-directed RNA polymerase subunit omega, at 2,395,843 to 2,396,046
_RS10640
HSERO_RS10645: HSERO_RS10645 - guanylate kinase, at 2,396,106 to 2,396,744
_RS10645
HSERO_RS10650: HSERO_RS10650 - hypothetical protein, at 2,396,830 to 2,397,729
_RS10650
HSERO_RS10655: HSERO_RS10655 - ribonuclease PH, at 2,398,069 to 2,398,797
_RS10655
Position (kb)
2396
2397
2398 Strain fitness (log2 ratio)
-1
0
1 at 2396.779 kb on - strand at 2397.288 kb on - strand, within HSERO_RS10650 at 2397.341 kb on - strand, within HSERO_RS10650 at 2397.722 kb on - strand at 2397.935 kb on - strand at 2398.055 kb on + strand at 2398.165 kb on - strand, within HSERO_RS10655 at 2398.165 kb on - strand, within HSERO_RS10655 at 2398.243 kb on + strand, within HSERO_RS10655 at 2398.297 kb on + strand, within HSERO_RS10655 at 2398.390 kb on - strand, within HSERO_RS10655 at 2398.688 kb on + strand, within HSERO_RS10655
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 2,396,779 - +0.1 2,397,288 - HSERO_RS10650 0.51 -1.0 2,397,341 - HSERO_RS10650 0.57 -0.5 2,397,722 - -0.6 2,397,935 - +0.3 2,398,055 + +0.6 2,398,165 - HSERO_RS10655 0.13 +0.3 2,398,165 - HSERO_RS10655 0.13 +0.8 2,398,243 + HSERO_RS10655 0.24 +0.2 2,398,297 + HSERO_RS10655 0.31 -0.5 2,398,390 - HSERO_RS10655 0.44 -0.7 2,398,688 + HSERO_RS10655 0.85 -0.4
Or see this region's nucleotide sequence