Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS08625 and HSERO_RS08630 are separated by 95 nucleotides HSERO_RS08630 and HSERO_RS08635 are separated by 241 nucleotides HSERO_RS08635 and HSERO_RS08640 are separated by 4 nucleotides HSERO_RS08640 and HSERO_RS08645 overlap by 4 nucleotides
HSERO_RS08625: HSERO_RS08625 - lipase, at 1,959,449 to 1,960,378
_RS08625
HSERO_RS08630: HSERO_RS08630 - membrane protein, at 1,960,474 to 1,960,980
_RS08630
HSERO_RS08635: HSERO_RS08635 - hypothetical protein, at 1,961,222 to 1,962,100
_RS08635
HSERO_RS08640: HSERO_RS08640 - hypothetical protein, at 1,962,105 to 1,962,515
_RS08640
HSERO_RS08645: HSERO_RS08645 - RNA polymerase sigma70 factor, at 1,962,512 to 1,963,090
_RS08645
Position (kb) 1961
1962
1963 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1960.399 kb on + strand at 1960.432 kb on + strand at 1960.437 kb on + strand at 1960.445 kb on - strand at 1960.510 kb on + strand at 1960.563 kb on + strand, within HSERO_RS08630 at 1960.564 kb on - strand, within HSERO_RS08630 at 1960.809 kb on + strand, within HSERO_RS08630 at 1961.002 kb on + strand at 1961.169 kb on + strand at 1961.177 kb on - strand at 1961.486 kb on - strand, within HSERO_RS08635 at 1961.486 kb on - strand, within HSERO_RS08635 at 1961.486 kb on - strand, within HSERO_RS08635 at 1961.766 kb on + strand, within HSERO_RS08635 at 1961.788 kb on + strand, within HSERO_RS08635 at 1962.011 kb on + strand, within HSERO_RS08635 at 1962.151 kb on - strand, within HSERO_RS08640 at 1962.373 kb on + strand, within HSERO_RS08640 at 1962.711 kb on - strand, within HSERO_RS08645 at 1962.711 kb on - strand, within HSERO_RS08645 at 1962.759 kb on - strand, within HSERO_RS08645 at 1962.787 kb on + strand, within HSERO_RS08645 at 1962.865 kb on + strand, within HSERO_RS08645 at 1963.030 kb on + strand, within HSERO_RS08645 at 1963.091 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 1,960,399 + -0.5 1,960,432 + +0.3 1,960,437 + -0.7 1,960,445 - +0.6 1,960,510 + -0.3 1,960,563 + HSERO_RS08630 0.18 -0.1 1,960,564 - HSERO_RS08630 0.18 -0.3 1,960,809 + HSERO_RS08630 0.66 +0.3 1,961,002 + -1.0 1,961,169 + +0.9 1,961,177 - +0.7 1,961,486 - HSERO_RS08635 0.30 -0.9 1,961,486 - HSERO_RS08635 0.30 -0.5 1,961,486 - HSERO_RS08635 0.30 -1.3 1,961,766 + HSERO_RS08635 0.62 -4.0 1,961,788 + HSERO_RS08635 0.64 -1.7 1,962,011 + HSERO_RS08635 0.90 -1.1 1,962,151 - HSERO_RS08640 0.11 -2.4 1,962,373 + HSERO_RS08640 0.65 -2.4 1,962,711 - HSERO_RS08645 0.34 +0.1 1,962,711 - HSERO_RS08645 0.34 -0.1 1,962,759 - HSERO_RS08645 0.43 +0.2 1,962,787 + HSERO_RS08645 0.47 +0.4 1,962,865 + HSERO_RS08645 0.61 +0.5 1,963,030 + HSERO_RS08645 0.89 +0.9 1,963,091 + +1.0
Or see this region's nucleotide sequence