Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS07745 and HSERO_RS07750 are separated by 174 nucleotides HSERO_RS07750 and HSERO_RS07755 are separated by 128 nucleotides HSERO_RS07755 and HSERO_RS07760 are separated by 36 nucleotides
HSERO_RS07745: HSERO_RS07745 - glutathione S-transferase, at 1,775,063 to 1,775,692
_RS07745
HSERO_RS07750: HSERO_RS07750 - tRNA-His, at 1,775,867 to 1,775,942
_RS07750
HSERO_RS07755: HSERO_RS07755 - glutamate racemase, at 1,776,071 to 1,776,964
_RS07755
HSERO_RS07760: HSERO_RS07760 - fumarate hydratase, at 1,777,001 to 1,778,542
_RS07760
Position (kb)
1776
1777 Strain fitness (log2 ratio)
-2
-1
0
1 at 1775.163 kb on - strand, within HSERO_RS07745 at 1775.196 kb on + strand, within HSERO_RS07745 at 1775.228 kb on + strand, within HSERO_RS07745 at 1775.266 kb on - strand, within HSERO_RS07745 at 1775.278 kb on - strand, within HSERO_RS07745 at 1775.435 kb on - strand, within HSERO_RS07745 at 1775.453 kb on + strand, within HSERO_RS07745 at 1775.512 kb on - strand, within HSERO_RS07745 at 1775.581 kb on - strand, within HSERO_RS07745 at 1776.250 kb on + strand, within HSERO_RS07755 at 1776.346 kb on - strand, within HSERO_RS07755 at 1776.403 kb on - strand, within HSERO_RS07755 at 1776.812 kb on + strand, within HSERO_RS07755 at 1777.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 1,775,163 - HSERO_RS07745 0.16 -0.4 1,775,196 + HSERO_RS07745 0.21 -0.3 1,775,228 + HSERO_RS07745 0.26 -0.2 1,775,266 - HSERO_RS07745 0.32 +0.3 1,775,278 - HSERO_RS07745 0.34 +0.1 1,775,435 - HSERO_RS07745 0.59 -0.2 1,775,453 + HSERO_RS07745 0.62 -0.6 1,775,512 - HSERO_RS07745 0.71 -0.2 1,775,581 - HSERO_RS07745 0.82 +0.2 1,776,250 + HSERO_RS07755 0.20 -2.4 1,776,346 - HSERO_RS07755 0.31 -1.5 1,776,403 - HSERO_RS07755 0.37 -2.5 1,776,812 + HSERO_RS07755 0.83 -2.4 1,777,002 - -0.3
Or see this region's nucleotide sequence