Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05470 and HSERO_RS05475 are separated by 56 nucleotides HSERO_RS05475 and HSERO_RS05480 are separated by 158 nucleotides HSERO_RS05480 and HSERO_RS05485 are separated by 28 nucleotides
HSERO_RS05470: HSERO_RS05470 - permease, at 1,237,067 to 1,238,419
_RS05470
HSERO_RS05475: HSERO_RS05475 - gluconokinase, at 1,238,476 to 1,240,038
_RS05475
HSERO_RS05480: HSERO_RS05480 - gluconate 5-dehydrogenase, at 1,240,197 to 1,240,991
_RS05480
HSERO_RS05485: HSERO_RS05485 - phosphoesterase, at 1,241,020 to 1,242,057
_RS05485
Position (kb)
1238
1239
1240
1241 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1237.545 kb on + strand, within HSERO_RS05470 at 1237.734 kb on - strand, within HSERO_RS05470 at 1237.827 kb on + strand, within HSERO_RS05470 at 1237.827 kb on + strand, within HSERO_RS05470 at 1237.835 kb on - strand, within HSERO_RS05470 at 1237.927 kb on - strand at 1238.140 kb on - strand, within HSERO_RS05470 at 1238.333 kb on - strand at 1238.347 kb on - strand at 1238.381 kb on + strand at 1238.404 kb on + strand at 1238.478 kb on + strand at 1238.545 kb on + strand at 1238.569 kb on - strand at 1238.606 kb on - strand at 1238.653 kb on + strand, within HSERO_RS05475 at 1238.731 kb on + strand, within HSERO_RS05475 at 1238.731 kb on + strand, within HSERO_RS05475 at 1238.731 kb on + strand, within HSERO_RS05475 at 1238.731 kb on + strand, within HSERO_RS05475 at 1238.739 kb on - strand, within HSERO_RS05475 at 1238.739 kb on - strand, within HSERO_RS05475 at 1238.758 kb on + strand, within HSERO_RS05475 at 1238.781 kb on - strand, within HSERO_RS05475 at 1238.906 kb on - strand, within HSERO_RS05475 at 1239.262 kb on + strand, within HSERO_RS05475 at 1239.349 kb on - strand at 1239.400 kb on + strand, within HSERO_RS05475 at 1239.431 kb on + strand, within HSERO_RS05475 at 1239.494 kb on + strand, within HSERO_RS05475 at 1239.851 kb on + strand, within HSERO_RS05475 at 1240.125 kb on + strand at 1240.125 kb on + strand at 1240.132 kb on + strand at 1240.150 kb on - strand at 1240.309 kb on + strand, within HSERO_RS05480 at 1240.440 kb on - strand at 1240.442 kb on + strand, within HSERO_RS05480 at 1240.442 kb on + strand, within HSERO_RS05480 at 1240.450 kb on - strand, within HSERO_RS05480 at 1240.570 kb on + strand at 1240.583 kb on - strand, within HSERO_RS05480 at 1240.652 kb on + strand, within HSERO_RS05480 at 1240.743 kb on + strand, within HSERO_RS05480 at 1240.796 kb on + strand, within HSERO_RS05480 at 1240.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 1,237,545 + HSERO_RS05470 0.35 +1.4 1,237,734 - HSERO_RS05470 0.49 -0.1 1,237,827 + HSERO_RS05470 0.56 +1.3 1,237,827 + HSERO_RS05470 0.56 -0.4 1,237,835 - HSERO_RS05470 0.57 +2.7 1,237,927 - +0.9 1,238,140 - HSERO_RS05470 0.79 -0.4 1,238,333 - -0.3 1,238,347 - +0.0 1,238,381 + -0.4 1,238,404 + +1.2 1,238,478 + -1.4 1,238,545 + +0.0 1,238,569 - -1.1 1,238,606 - -1.3 1,238,653 + HSERO_RS05475 0.11 -1.9 1,238,731 + HSERO_RS05475 0.16 -0.5 1,238,731 + HSERO_RS05475 0.16 -0.7 1,238,731 + HSERO_RS05475 0.16 -1.8 1,238,731 + HSERO_RS05475 0.16 +1.3 1,238,739 - HSERO_RS05475 0.17 -0.5 1,238,739 - HSERO_RS05475 0.17 -1.3 1,238,758 + HSERO_RS05475 0.18 +0.1 1,238,781 - HSERO_RS05475 0.20 -0.6 1,238,906 - HSERO_RS05475 0.28 -1.0 1,239,262 + HSERO_RS05475 0.50 -1.2 1,239,349 - +0.3 1,239,400 + HSERO_RS05475 0.59 -1.3 1,239,431 + HSERO_RS05475 0.61 -1.3 1,239,494 + HSERO_RS05475 0.65 -1.2 1,239,851 + HSERO_RS05475 0.88 -1.2 1,240,125 + -0.1 1,240,125 + -0.5 1,240,132 + +0.7 1,240,150 - -0.8 1,240,309 + HSERO_RS05480 0.14 -0.4 1,240,440 - -0.6 1,240,442 + HSERO_RS05480 0.31 +0.2 1,240,442 + HSERO_RS05480 0.31 -0.1 1,240,450 - HSERO_RS05480 0.32 +0.3 1,240,570 + -1.5 1,240,583 - HSERO_RS05480 0.49 -1.1 1,240,652 + HSERO_RS05480 0.57 -0.7 1,240,743 + HSERO_RS05480 0.69 -0.8 1,240,796 + HSERO_RS05480 0.75 -0.0 1,240,937 - -0.2
Or see this region's nucleotide sequence