Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05120 and HSERO_RS05125 are separated by 262 nucleotides HSERO_RS05125 and HSERO_RS05130 are separated by 23 nucleotides HSERO_RS05130 and HSERO_RS05135 are separated by 101 nucleotides
HSERO_RS05120: HSERO_RS05120 - deoxyribose-phosphate aldolase, at 1,132,326 to 1,133,357
_RS05120
HSERO_RS05125: HSERO_RS05125 - DeoR family transcripitonal regulator, at 1,133,620 to 1,134,435
_RS05125
HSERO_RS05130: HSERO_RS05130 - methionine aminopeptidase, at 1,134,459 to 1,135,256
_RS05130
HSERO_RS05135: HSERO_RS05135 - ABC transporter permease, at 1,135,358 to 1,136,230
_RS05135
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-2
-1
0
1 at 1132.717 kb on + strand, within HSERO_RS05120 at 1132.725 kb on - strand, within HSERO_RS05120 at 1132.767 kb on + strand, within HSERO_RS05120 at 1132.879 kb on - strand, within HSERO_RS05120 at 1132.999 kb on + strand at 1133.114 kb on - strand, within HSERO_RS05120 at 1133.287 kb on + strand at 1133.316 kb on - strand at 1133.338 kb on - strand at 1133.352 kb on + strand at 1133.398 kb on + strand at 1133.443 kb on + strand at 1133.443 kb on + strand at 1133.734 kb on - strand, within HSERO_RS05125 at 1133.846 kb on + strand, within HSERO_RS05125 at 1133.854 kb on - strand, within HSERO_RS05125 at 1133.894 kb on + strand, within HSERO_RS05125 at 1133.894 kb on + strand, within HSERO_RS05125 at 1133.978 kb on - strand, within HSERO_RS05125 at 1134.308 kb on - strand, within HSERO_RS05125 at 1134.817 kb on + strand, within HSERO_RS05130 at 1134.988 kb on + strand, within HSERO_RS05130 at 1135.059 kb on - strand, within HSERO_RS05130 at 1135.116 kb on - strand, within HSERO_RS05130 at 1135.161 kb on - strand, within HSERO_RS05130 at 1135.161 kb on - strand, within HSERO_RS05130 at 1135.363 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 1,132,717 + HSERO_RS05120 0.38 +0.8 1,132,725 - HSERO_RS05120 0.39 -1.0 1,132,767 + HSERO_RS05120 0.43 -0.5 1,132,879 - HSERO_RS05120 0.54 -1.9 1,132,999 + +0.1 1,133,114 - HSERO_RS05120 0.76 -0.0 1,133,287 + -0.5 1,133,316 - -0.7 1,133,338 - +0.6 1,133,352 + +0.0 1,133,398 + -1.0 1,133,443 + -0.9 1,133,443 + -0.5 1,133,734 - HSERO_RS05125 0.14 -2.1 1,133,846 + HSERO_RS05125 0.28 -1.7 1,133,854 - HSERO_RS05125 0.29 -1.7 1,133,894 + HSERO_RS05125 0.34 -1.6 1,133,894 + HSERO_RS05125 0.34 -0.6 1,133,978 - HSERO_RS05125 0.44 -1.0 1,134,308 - HSERO_RS05125 0.84 -1.5 1,134,817 + HSERO_RS05130 0.45 -0.5 1,134,988 + HSERO_RS05130 0.66 +0.4 1,135,059 - HSERO_RS05130 0.75 -0.4 1,135,116 - HSERO_RS05130 0.82 -0.0 1,135,161 - HSERO_RS05130 0.88 +0.8 1,135,161 - HSERO_RS05130 0.88 -0.0 1,135,363 - -0.7
Or see this region's nucleotide sequence