Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04860 and HSERO_RS04865 are separated by 2 nucleotides HSERO_RS04865 and HSERO_RS04870 are separated by 280 nucleotides HSERO_RS04870 and HSERO_RS04875 are separated by 270 nucleotides
HSERO_RS04860: HSERO_RS04860 - sugar ABC transporter permease, at 1,064,284 to 1,065,120
_RS04860
HSERO_RS04865: HSERO_RS04865 - membrane protein, at 1,065,123 to 1,065,407
_RS04865
HSERO_RS04870: HSERO_RS04870 - glycerol ABC transporter, substrate-binding component (from data) , at 1,065,688 to 1,067,436
_RS04870
HSERO_RS04875: HSERO_RS04875 - glycerol kinase, at 1,067,707 to 1,069,194
_RS04875
Position (kb)
1065
1066
1067
1068 Strain fitness (log2 ratio)
-2
-1
0
1 at 1064.881 kb on - strand at 1065.052 kb on - strand at 1065.555 kb on - strand at 1065.566 kb on - strand at 1065.623 kb on - strand at 1065.879 kb on + strand, within HSERO_RS04870 at 1066.024 kb on + strand, within HSERO_RS04870 at 1066.209 kb on + strand, within HSERO_RS04870 at 1066.233 kb on + strand, within HSERO_RS04870 at 1066.257 kb on - strand, within HSERO_RS04870 at 1066.272 kb on - strand, within HSERO_RS04870 at 1066.359 kb on - strand, within HSERO_RS04870 at 1066.739 kb on - strand, within HSERO_RS04870 at 1066.838 kb on + strand at 1066.907 kb on + strand, within HSERO_RS04870 at 1066.979 kb on - strand, within HSERO_RS04870 at 1067.011 kb on + strand, within HSERO_RS04870 at 1067.163 kb on - strand, within HSERO_RS04870 at 1067.228 kb on - strand, within HSERO_RS04870 at 1067.288 kb on + strand at 1067.543 kb on + strand at 1067.580 kb on - strand at 1067.580 kb on - strand at 1067.649 kb on - strand at 1067.868 kb on + strand, within HSERO_RS04875 at 1067.936 kb on + strand, within HSERO_RS04875 at 1067.962 kb on - strand, within HSERO_RS04875 at 1068.043 kb on - strand, within HSERO_RS04875 at 1068.239 kb on + strand, within HSERO_RS04875
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 1,064,881 - +0.3 1,065,052 - -0.3 1,065,555 - -0.5 1,065,566 - +1.3 1,065,623 - -0.5 1,065,879 + HSERO_RS04870 0.11 -0.8 1,066,024 + HSERO_RS04870 0.19 -0.8 1,066,209 + HSERO_RS04870 0.30 -0.9 1,066,233 + HSERO_RS04870 0.31 -0.8 1,066,257 - HSERO_RS04870 0.33 -0.8 1,066,272 - HSERO_RS04870 0.33 +0.1 1,066,359 - HSERO_RS04870 0.38 -1.4 1,066,739 - HSERO_RS04870 0.60 -0.3 1,066,838 + -0.1 1,066,907 + HSERO_RS04870 0.70 -1.0 1,066,979 - HSERO_RS04870 0.74 -0.3 1,067,011 + HSERO_RS04870 0.76 -1.0 1,067,163 - HSERO_RS04870 0.84 +0.9 1,067,228 - HSERO_RS04870 0.88 -0.8 1,067,288 + -1.5 1,067,543 + -0.2 1,067,580 - -0.2 1,067,580 - -0.5 1,067,649 - -0.4 1,067,868 + HSERO_RS04875 0.11 -2.6 1,067,936 + HSERO_RS04875 0.15 -2.2 1,067,962 - HSERO_RS04875 0.17 -1.0 1,068,043 - HSERO_RS04875 0.23 +0.3 1,068,239 + HSERO_RS04875 0.36 -0.9
Or see this region's nucleotide sequence