Experiment: LB, pH5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_342 and Ac3H11_343 are separated by 11 nucleotides Ac3H11_343 and Ac3H11_344 are separated by 88 nucleotides Ac3H11_344 and Ac3H11_345 are separated by 228 nucleotides Ac3H11_345 and Ac3H11_346 overlap by 4 nucleotides
Ac3H11_342 - Osmolarity sensory histidine kinase EnvZ, at 19,071 to 20,594
_342
Ac3H11_343 - Two-component system response regulator OmpR, at 20,606 to 21,337
_343
Ac3H11_344 - hypothetical protein, at 21,426 to 21,599
_344
Ac3H11_345 - hypothetical protein, at 21,828 to 22,223
_345
Ac3H11_346 - hypothetical protein, at 22,220 to 22,435
_346
Position (kb) 21
22 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 21.428 kb on - strand at 21.810 kb on - strand at 22.033 kb on - strand, within Ac3H11_345 at 22.059 kb on + strand, within Ac3H11_345 at 22.060 kb on - strand, within Ac3H11_345 at 22.325 kb on + strand, within Ac3H11_346 at 22.326 kb on - strand, within Ac3H11_346 at 22.356 kb on - strand, within Ac3H11_346 at 22.356 kb on - strand, within Ac3H11_346 at 22.356 kb on - strand, within Ac3H11_346 at 22.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH5 remove 20,591 - -0.8 20,591 - +0.0 20,591 - +0.7 20,591 - +0.8 20,591 - +0.1 20,591 - -0.0 20,591 - +1.1 20,591 - +1.1 20,591 - +0.7 20,591 - +1.0 21,428 - -3.8 21,810 - -2.6 22,033 - Ac3H11_345 0.52 -0.2 22,059 + Ac3H11_345 0.58 -1.3 22,060 - Ac3H11_345 0.59 -1.3 22,325 + Ac3H11_346 0.49 +0.0 22,326 - Ac3H11_346 0.49 -0.2 22,356 - Ac3H11_346 0.63 +0.1 22,356 - Ac3H11_346 0.63 +0.2 22,356 - Ac3H11_346 0.63 +0.6 22,520 - -0.4
Or see this region's nucleotide sequence