Experiment: casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01715 and HSERO_RS01720 are separated by 186 nucleotides HSERO_RS01720 and HSERO_RS01725 are separated by 13 nucleotides HSERO_RS01725 and HSERO_RS01730 are separated by 35 nucleotides
HSERO_RS01715: HSERO_RS01715 - preprotein translocase subunit SecA, at 381,850 to 384,606
_RS01715
HSERO_RS01720: HSERO_RS01720 - membrane protein, at 384,793 to 385,587
_RS01720
HSERO_RS01725: HSERO_RS01725 - lysophospholipase, at 385,601 to 386,353
_RS01725
HSERO_RS01730: HSERO_RS01730 - N-acetylglutamate synthase, at 386,389 to 387,624
_RS01730
Position (kb) 385
386
387 Strain fitness (log2 ratio) -1
0
1 at 384.729 kb on + strand at 384.819 kb on - strand at 384.907 kb on + strand, within HSERO_RS01720 at 385.465 kb on - strand, within HSERO_RS01720 at 385.643 kb on + strand at 385.678 kb on - strand, within HSERO_RS01725 at 385.762 kb on + strand, within HSERO_RS01725 at 385.957 kb on + strand, within HSERO_RS01725 at 386.261 kb on + strand, within HSERO_RS01725 at 386.265 kb on - strand, within HSERO_RS01725 at 386.283 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos remove 384,729 + +0.3 384,819 - +0.3 384,907 + HSERO_RS01720 0.14 +0.6 385,465 - HSERO_RS01720 0.85 -0.6 385,643 + +1.1 385,678 - HSERO_RS01725 0.10 -0.3 385,762 + HSERO_RS01725 0.21 +0.5 385,957 + HSERO_RS01725 0.47 +0.3 386,261 + HSERO_RS01725 0.88 +0.6 386,265 - HSERO_RS01725 0.88 +0.7 386,283 + +0.2
Or see this region's nucleotide sequence