Experiment: Phosphomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mreB and ssb are separated by 192 nucleotides ssb and Synpcc7942_0302 are separated by 17 nucleotides Synpcc7942_0302 and Synpcc7942_0303 are separated by 95 nucleotides
Synpcc7942_0300: mreB - rod shape-determining protein MreB, at 298,398 to 299,435
mreB
Synpcc7942_0301: ssb - single-strand DNA-binding protein, at 299,628 to 299,990
ssb
Synpcc7942_0302: Synpcc7942_0302 - Phospholipase D/Transphosphatidylase, at 300,008 to 301,447
_0302
Synpcc7942_0303: Synpcc7942_0303 - response regulator receiver domain protein (CheY-like), at 301,543 to 302,697
_0303
Position (kb)
300
301
302 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 299.608 kb on - strand at 299.608 kb on - strand at 299.608 kb on - strand at 299.608 kb on - strand at 299.612 kb on + strand at 299.613 kb on + strand at 299.615 kb on - strand at 299.634 kb on - strand at 299.914 kb on + strand, within ssb at 299.914 kb on + strand, within ssb at 299.946 kb on - strand, within ssb at 299.961 kb on - strand at 299.984 kb on + strand at 299.992 kb on - strand at 299.992 kb on - strand at 299.992 kb on - strand at 300.019 kb on - strand at 300.026 kb on - strand at 300.049 kb on - strand at 300.100 kb on - strand at 300.121 kb on + strand at 300.126 kb on + strand at 300.126 kb on + strand at 300.126 kb on + strand at 300.126 kb on + strand at 300.126 kb on + strand at 300.126 kb on + strand at 300.134 kb on - strand at 300.134 kb on - strand at 300.134 kb on - strand at 300.134 kb on - strand at 300.134 kb on - strand at 300.134 kb on - strand at 300.134 kb on - strand at 300.134 kb on - strand at 300.134 kb on - strand at 300.145 kb on - strand at 300.214 kb on - strand, within Synpcc7942_0302 at 300.230 kb on - strand, within Synpcc7942_0302 at 300.244 kb on + strand, within Synpcc7942_0302 at 300.257 kb on - strand, within Synpcc7942_0302 at 300.318 kb on + strand, within Synpcc7942_0302 at 300.318 kb on + strand, within Synpcc7942_0302 at 300.333 kb on + strand, within Synpcc7942_0302 at 300.333 kb on + strand, within Synpcc7942_0302 at 300.341 kb on - strand, within Synpcc7942_0302 at 300.347 kb on + strand, within Synpcc7942_0302 at 300.357 kb on + strand, within Synpcc7942_0302 at 300.379 kb on + strand, within Synpcc7942_0302 at 300.379 kb on + strand, within Synpcc7942_0302 at 300.379 kb on + strand, within Synpcc7942_0302 at 300.379 kb on + strand, within Synpcc7942_0302 at 300.379 kb on + strand, within Synpcc7942_0302 at 300.387 kb on - strand, within Synpcc7942_0302 at 300.387 kb on - strand, within Synpcc7942_0302 at 300.395 kb on + strand, within Synpcc7942_0302 at 300.398 kb on - strand, within Synpcc7942_0302 at 300.403 kb on - strand, within Synpcc7942_0302 at 300.416 kb on - strand, within Synpcc7942_0302 at 300.428 kb on + strand, within Synpcc7942_0302 at 300.440 kb on - strand, within Synpcc7942_0302 at 300.451 kb on + strand, within Synpcc7942_0302 at 300.451 kb on + strand, within Synpcc7942_0302 at 300.459 kb on - strand, within Synpcc7942_0302 at 300.466 kb on - strand, within Synpcc7942_0302 at 300.494 kb on + strand, within Synpcc7942_0302 at 300.509 kb on + strand, within Synpcc7942_0302 at 300.517 kb on + strand, within Synpcc7942_0302 at 300.517 kb on + strand, within Synpcc7942_0302 at 300.517 kb on + strand, within Synpcc7942_0302 at 300.522 kb on + strand, within Synpcc7942_0302 at 300.544 kb on + strand, within Synpcc7942_0302 at 300.573 kb on + strand, within Synpcc7942_0302 at 300.612 kb on + strand, within Synpcc7942_0302 at 300.634 kb on + strand, within Synpcc7942_0302 at 300.648 kb on + strand, within Synpcc7942_0302 at 300.665 kb on + strand, within Synpcc7942_0302 at 300.666 kb on + strand, within Synpcc7942_0302 at 300.684 kb on - strand, within Synpcc7942_0302 at 300.714 kb on + strand, within Synpcc7942_0302 at 300.714 kb on + strand, within Synpcc7942_0302 at 300.762 kb on - strand, within Synpcc7942_0302 at 300.773 kb on + strand, within Synpcc7942_0302 at 300.855 kb on + strand, within Synpcc7942_0302 at 300.855 kb on + strand, within Synpcc7942_0302 at 300.861 kb on + strand, within Synpcc7942_0302 at 300.885 kb on + strand, within Synpcc7942_0302 at 300.885 kb on + strand, within Synpcc7942_0302 at 300.893 kb on - strand, within Synpcc7942_0302 at 300.895 kb on - strand, within Synpcc7942_0302 at 300.916 kb on - strand, within Synpcc7942_0302 at 300.932 kb on + strand, within Synpcc7942_0302 at 300.943 kb on + strand, within Synpcc7942_0302 at 300.954 kb on - strand, within Synpcc7942_0302 at 300.962 kb on + strand, within Synpcc7942_0302 at 300.964 kb on - strand, within Synpcc7942_0302 at 300.996 kb on + strand, within Synpcc7942_0302 at 301.014 kb on + strand, within Synpcc7942_0302 at 301.019 kb on + strand, within Synpcc7942_0302 at 301.074 kb on - strand, within Synpcc7942_0302 at 301.076 kb on + strand, within Synpcc7942_0302 at 301.084 kb on - strand, within Synpcc7942_0302 at 301.091 kb on + strand, within Synpcc7942_0302 at 301.098 kb on + strand, within Synpcc7942_0302 at 301.099 kb on - strand, within Synpcc7942_0302 at 301.099 kb on - strand, within Synpcc7942_0302 at 301.118 kb on - strand, within Synpcc7942_0302 at 301.215 kb on + strand, within Synpcc7942_0302 at 301.220 kb on + strand, within Synpcc7942_0302 at 301.225 kb on + strand, within Synpcc7942_0302 at 301.225 kb on + strand, within Synpcc7942_0302 at 301.285 kb on - strand, within Synpcc7942_0302 at 301.300 kb on + strand, within Synpcc7942_0302 at 301.333 kb on - strand at 301.356 kb on + strand at 301.356 kb on + strand at 301.369 kb on - strand at 301.369 kb on - strand at 301.374 kb on + strand at 301.432 kb on + strand at 301.434 kb on + strand at 301.454 kb on - strand at 301.454 kb on - strand at 301.454 kb on - strand at 301.454 kb on - strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.456 kb on + strand at 301.464 kb on - strand at 301.466 kb on + strand at 301.466 kb on + strand at 301.470 kb on + strand at 301.474 kb on - strand at 301.474 kb on - strand at 301.484 kb on - strand at 301.494 kb on + strand at 301.524 kb on - strand at 301.524 kb on - strand at 301.526 kb on + strand at 301.527 kb on + strand at 301.545 kb on + strand at 301.546 kb on - strand at 301.548 kb on + strand at 301.550 kb on + strand at 301.551 kb on - strand at 301.553 kb on - strand at 301.596 kb on - strand at 301.632 kb on - strand at 301.640 kb on + strand at 301.658 kb on - strand at 301.658 kb on - strand at 301.665 kb on - strand, within Synpcc7942_0303 at 301.696 kb on + strand, within Synpcc7942_0303 at 301.737 kb on - strand, within Synpcc7942_0303 at 301.798 kb on + strand, within Synpcc7942_0303 at 301.798 kb on + strand, within Synpcc7942_0303 at 301.805 kb on + strand, within Synpcc7942_0303 at 301.805 kb on + strand, within Synpcc7942_0303 at 301.806 kb on - strand, within Synpcc7942_0303 at 301.867 kb on - strand, within Synpcc7942_0303 at 301.887 kb on + strand, within Synpcc7942_0303 at 301.895 kb on - strand, within Synpcc7942_0303 at 301.915 kb on + strand, within Synpcc7942_0303 at 301.962 kb on - strand, within Synpcc7942_0303 at 301.966 kb on + strand, within Synpcc7942_0303 at 301.993 kb on + strand, within Synpcc7942_0303 at 302.001 kb on - strand, within Synpcc7942_0303 at 302.001 kb on - strand, within Synpcc7942_0303 at 302.001 kb on - strand, within Synpcc7942_0303 at 302.025 kb on - strand, within Synpcc7942_0303 at 302.108 kb on + strand, within Synpcc7942_0303 at 302.116 kb on - strand, within Synpcc7942_0303 at 302.116 kb on - strand, within Synpcc7942_0303 at 302.116 kb on - strand, within Synpcc7942_0303 at 302.116 kb on - strand, within Synpcc7942_0303 at 302.118 kb on - strand, within Synpcc7942_0303 at 302.128 kb on - strand, within Synpcc7942_0303 at 302.140 kb on + strand, within Synpcc7942_0303 at 302.143 kb on - strand, within Synpcc7942_0303 at 302.148 kb on - strand, within Synpcc7942_0303 at 302.148 kb on - strand, within Synpcc7942_0303 at 302.148 kb on - strand, within Synpcc7942_0303 at 302.148 kb on - strand, within Synpcc7942_0303 at 302.165 kb on + strand at 302.169 kb on + strand, within Synpcc7942_0303 at 302.211 kb on - strand, within Synpcc7942_0303 at 302.249 kb on + strand, within Synpcc7942_0303 at 302.281 kb on + strand, within Synpcc7942_0303 at 302.286 kb on + strand, within Synpcc7942_0303 at 302.305 kb on + strand, within Synpcc7942_0303 at 302.313 kb on - strand, within Synpcc7942_0303 at 302.313 kb on - strand, within Synpcc7942_0303 at 302.331 kb on + strand, within Synpcc7942_0303 at 302.351 kb on - strand, within Synpcc7942_0303 at 302.351 kb on - strand, within Synpcc7942_0303 at 302.363 kb on + strand, within Synpcc7942_0303 at 302.398 kb on + strand, within Synpcc7942_0303 at 302.398 kb on + strand, within Synpcc7942_0303
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.00625 mg/ml remove 299,608 - +0.2 299,608 - +0.2 299,608 - +0.3 299,608 - +2.5 299,612 + +0.4 299,613 + -1.3 299,615 - +1.0 299,634 - -0.0 299,914 + ssb Synpcc7942_0301 0.79 -1.1 299,914 + ssb Synpcc7942_0301 0.79 -0.8 299,946 - ssb Synpcc7942_0301 0.88 +1.2 299,961 - +1.5 299,984 + +1.1 299,992 - +0.4 299,992 - -0.5 299,992 - +1.2 300,019 - -0.1 300,026 - -0.7 300,049 - +0.9 300,100 - -0.7 300,121 + -1.7 300,126 + +0.1 300,126 + -1.1 300,126 + +0.6 300,126 + -0.6 300,126 + +0.3 300,126 + -0.1 300,134 - +0.2 300,134 - -1.5 300,134 - -0.3 300,134 - +0.7 300,134 - -0.5 300,134 - -0.7 300,134 - -1.4 300,134 - +0.3 300,134 - -2.0 300,145 - -1.2 300,214 - Synpcc7942_0302 0.14 +1.5 300,230 - Synpcc7942_0302 0.15 +0.3 300,244 + Synpcc7942_0302 0.16 -0.1 300,257 - Synpcc7942_0302 0.17 -0.6 300,318 + Synpcc7942_0302 0.22 +0.1 300,318 + Synpcc7942_0302 0.22 -0.2 300,333 + Synpcc7942_0302 0.23 +0.0 300,333 + Synpcc7942_0302 0.23 -0.0 300,341 - Synpcc7942_0302 0.23 -0.1 300,347 + Synpcc7942_0302 0.24 -1.3 300,357 + Synpcc7942_0302 0.24 +0.2 300,379 + Synpcc7942_0302 0.26 -1.1 300,379 + Synpcc7942_0302 0.26 -0.6 300,379 + Synpcc7942_0302 0.26 +0.7 300,379 + Synpcc7942_0302 0.26 -1.1 300,379 + Synpcc7942_0302 0.26 -0.6 300,387 - Synpcc7942_0302 0.26 -0.5 300,387 - Synpcc7942_0302 0.26 -0.7 300,395 + Synpcc7942_0302 0.27 +0.2 300,398 - Synpcc7942_0302 0.27 -0.8 300,403 - Synpcc7942_0302 0.27 -0.7 300,416 - Synpcc7942_0302 0.28 +0.5 300,428 + Synpcc7942_0302 0.29 +0.1 300,440 - Synpcc7942_0302 0.30 -0.5 300,451 + Synpcc7942_0302 0.31 -0.7 300,451 + Synpcc7942_0302 0.31 -0.7 300,459 - Synpcc7942_0302 0.31 +0.8 300,466 - Synpcc7942_0302 0.32 +1.7 300,494 + Synpcc7942_0302 0.34 +0.1 300,509 + Synpcc7942_0302 0.35 -0.8 300,517 + Synpcc7942_0302 0.35 -0.3 300,517 + Synpcc7942_0302 0.35 +0.3 300,517 + Synpcc7942_0302 0.35 -0.6 300,522 + Synpcc7942_0302 0.36 -0.2 300,544 + Synpcc7942_0302 0.37 +0.8 300,573 + Synpcc7942_0302 0.39 -0.5 300,612 + Synpcc7942_0302 0.42 +0.4 300,634 + Synpcc7942_0302 0.43 +0.2 300,648 + Synpcc7942_0302 0.44 +0.9 300,665 + Synpcc7942_0302 0.46 +0.1 300,666 + Synpcc7942_0302 0.46 -0.4 300,684 - Synpcc7942_0302 0.47 +0.1 300,714 + Synpcc7942_0302 0.49 -1.4 300,714 + Synpcc7942_0302 0.49 +0.2 300,762 - Synpcc7942_0302 0.52 -0.1 300,773 + Synpcc7942_0302 0.53 +1.5 300,855 + Synpcc7942_0302 0.59 -0.5 300,855 + Synpcc7942_0302 0.59 -0.6 300,861 + Synpcc7942_0302 0.59 +0.2 300,885 + Synpcc7942_0302 0.61 -1.5 300,885 + Synpcc7942_0302 0.61 -0.1 300,893 - Synpcc7942_0302 0.61 -0.9 300,895 - Synpcc7942_0302 0.62 +0.4 300,916 - Synpcc7942_0302 0.63 +0.2 300,932 + Synpcc7942_0302 0.64 -2.1 300,943 + Synpcc7942_0302 0.65 +0.9 300,954 - Synpcc7942_0302 0.66 +1.2 300,962 + Synpcc7942_0302 0.66 -0.5 300,964 - Synpcc7942_0302 0.66 -1.3 300,996 + Synpcc7942_0302 0.69 -0.4 301,014 + Synpcc7942_0302 0.70 +0.8 301,019 + Synpcc7942_0302 0.70 +2.5 301,074 - Synpcc7942_0302 0.74 -0.8 301,076 + Synpcc7942_0302 0.74 -0.8 301,084 - Synpcc7942_0302 0.75 +0.4 301,091 + Synpcc7942_0302 0.75 -3.8 301,098 + Synpcc7942_0302 0.76 -0.1 301,099 - Synpcc7942_0302 0.76 +1.5 301,099 - Synpcc7942_0302 0.76 -0.1 301,118 - Synpcc7942_0302 0.77 -0.7 301,215 + Synpcc7942_0302 0.84 +1.2 301,220 + Synpcc7942_0302 0.84 -0.1 301,225 + Synpcc7942_0302 0.85 +0.2 301,225 + Synpcc7942_0302 0.85 -0.2 301,285 - Synpcc7942_0302 0.89 -0.1 301,300 + Synpcc7942_0302 0.90 +0.8 301,333 - +0.7 301,356 + -0.1 301,356 + +0.8 301,369 - +0.1 301,369 - -0.2 301,374 + -1.6 301,432 + -0.7 301,434 + +1.7 301,454 - +1.6 301,454 - -0.1 301,454 - +0.1 301,454 - +1.2 301,456 + -0.9 301,456 + +0.8 301,456 + +0.9 301,456 + -0.1 301,456 + -0.5 301,456 + -0.4 301,456 + +2.2 301,456 + +0.7 301,456 + +1.8 301,456 + +1.1 301,456 + +1.5 301,464 - -0.1 301,466 + +0.7 301,466 + +0.1 301,470 + -1.1 301,474 - -0.6 301,474 - -0.5 301,484 - +1.5 301,494 + +0.2 301,524 - +0.8 301,524 - +0.9 301,526 + +0.3 301,527 + +2.1 301,545 + +0.5 301,546 - +0.7 301,548 + +0.9 301,550 + -1.8 301,551 - -0.1 301,553 - -0.7 301,596 - -0.5 301,632 - +0.9 301,640 + -0.1 301,658 - +0.2 301,658 - -0.1 301,665 - Synpcc7942_0303 0.11 +0.3 301,696 + Synpcc7942_0303 0.13 -1.4 301,737 - Synpcc7942_0303 0.17 +2.9 301,798 + Synpcc7942_0303 0.22 -1.1 301,798 + Synpcc7942_0303 0.22 +0.8 301,805 + Synpcc7942_0303 0.23 -0.1 301,805 + Synpcc7942_0303 0.23 -0.4 301,806 - Synpcc7942_0303 0.23 -0.8 301,867 - Synpcc7942_0303 0.28 +0.3 301,887 + Synpcc7942_0303 0.30 +0.7 301,895 - Synpcc7942_0303 0.30 -1.7 301,915 + Synpcc7942_0303 0.32 +3.5 301,962 - Synpcc7942_0303 0.36 +0.0 301,966 + Synpcc7942_0303 0.37 -0.1 301,993 + Synpcc7942_0303 0.39 +0.5 302,001 - Synpcc7942_0303 0.40 +0.2 302,001 - Synpcc7942_0303 0.40 -0.1 302,001 - Synpcc7942_0303 0.40 -1.4 302,025 - Synpcc7942_0303 0.42 +0.5 302,108 + Synpcc7942_0303 0.49 -0.5 302,116 - Synpcc7942_0303 0.50 -0.2 302,116 - Synpcc7942_0303 0.50 +1.2 302,116 - Synpcc7942_0303 0.50 -0.5 302,116 - Synpcc7942_0303 0.50 +0.3 302,118 - Synpcc7942_0303 0.50 -0.3 302,128 - Synpcc7942_0303 0.51 -0.8 302,140 + Synpcc7942_0303 0.52 +0.5 302,143 - Synpcc7942_0303 0.52 -0.1 302,148 - Synpcc7942_0303 0.52 +0.2 302,148 - Synpcc7942_0303 0.52 +0.6 302,148 - Synpcc7942_0303 0.52 -0.3 302,148 - Synpcc7942_0303 0.52 -1.7 302,165 + -1.1 302,169 + Synpcc7942_0303 0.54 -0.6 302,211 - Synpcc7942_0303 0.58 +0.1 302,249 + Synpcc7942_0303 0.61 -0.1 302,281 + Synpcc7942_0303 0.64 -0.3 302,286 + Synpcc7942_0303 0.64 -0.1 302,305 + Synpcc7942_0303 0.66 -0.1 302,313 - Synpcc7942_0303 0.67 -0.7 302,313 - Synpcc7942_0303 0.67 -0.8 302,331 + Synpcc7942_0303 0.68 +0.6 302,351 - Synpcc7942_0303 0.70 -1.7 302,351 - Synpcc7942_0303 0.70 +1.1 302,363 + Synpcc7942_0303 0.71 +0.5 302,398 + Synpcc7942_0303 0.74 -0.1 302,398 + Synpcc7942_0303 0.74 +1.8
Or see this region's nucleotide sequence