Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3163 and DvMF_3164 are separated by 239 nucleotides DvMF_3164 and DvMF_3165 are separated by 272 nucleotides DvMF_3165 and DvMF_3166 are separated by 10 nucleotides
DvMF_3163: DvMF_3163 - signal peptide peptidase SppA, 36K type (RefSeq), at 3,989,316 to 3,990,323
_3163
DvMF_3164: DvMF_3164 - Cobyrinic acid ac-diamide synthase (RefSeq), at 3,990,563 to 3,991,516
_3164
DvMF_3165: DvMF_3165 - hydrogenase accessory protein HypB (RefSeq), at 3,991,789 to 3,992,445
_3165
DvMF_3166: DvMF_3166 - hydrogenase expression/synthesis HypA (RefSeq), at 3,992,456 to 3,992,800
_3166
Position (kb)
3990
3991
3992 Strain fitness (log2 ratio)
-2
-1
0
1 at 3989.745 kb on + strand, within DvMF_3163 at 3989.796 kb on - strand, within DvMF_3163 at 3989.854 kb on + strand, within DvMF_3163 at 3990.185 kb on - strand, within DvMF_3163 at 3990.247 kb on + strand at 3990.806 kb on - strand, within DvMF_3164 at 3991.426 kb on + strand at 3991.552 kb on - strand at 3991.559 kb on + strand at 3991.782 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 3,989,745 + DvMF_3163 0.43 -0.2 3,989,796 - DvMF_3163 0.48 -0.6 3,989,854 + DvMF_3163 0.53 -1.3 3,990,185 - DvMF_3163 0.86 -0.8 3,990,247 + -0.2 3,990,806 - DvMF_3164 0.25 -2.1 3,991,426 + -0.2 3,991,552 - -0.8 3,991,559 + -0.6 3,991,782 - -0.4
Or see this region's nucleotide sequence