Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_979 and Pf1N1B4_980 are separated by 48 nucleotides Pf1N1B4_980 and Pf1N1B4_981 are separated by 60 nucleotides Pf1N1B4_981 and Pf1N1B4_982 are separated by 820 nucleotides
Pf1N1B4_979 - Uricase (urate oxidase) (EC 1.7.3.3), at 1,107,170 to 1,108,294
_979
Pf1N1B4_980 - hypothetical protein, at 1,108,343 to 1,108,471
_980
Pf1N1B4_981 - UPF0246 protein YaaA, at 1,108,532 to 1,109,311
_981
Pf1N1B4_982 - GDP-mannose 6-dehydrogenase (EC 1.1.1.132), at 1,110,132 to 1,111,448
_982
Position (kb)
1108
1109
1110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1107.582 kb on + strand, within Pf1N1B4_979 at 1107.582 kb on + strand, within Pf1N1B4_979 at 1107.582 kb on + strand, within Pf1N1B4_979 at 1107.582 kb on + strand, within Pf1N1B4_979 at 1107.583 kb on - strand, within Pf1N1B4_979 at 1107.583 kb on - strand, within Pf1N1B4_979 at 1107.583 kb on - strand, within Pf1N1B4_979 at 1107.655 kb on + strand, within Pf1N1B4_979 at 1107.656 kb on - strand, within Pf1N1B4_979 at 1107.681 kb on + strand, within Pf1N1B4_979 at 1107.682 kb on - strand, within Pf1N1B4_979 at 1107.703 kb on + strand, within Pf1N1B4_979 at 1107.704 kb on - strand, within Pf1N1B4_979 at 1107.704 kb on - strand, within Pf1N1B4_979 at 1107.704 kb on - strand, within Pf1N1B4_979 at 1107.780 kb on + strand, within Pf1N1B4_979 at 1107.852 kb on + strand, within Pf1N1B4_979 at 1107.894 kb on + strand, within Pf1N1B4_979 at 1107.894 kb on + strand, within Pf1N1B4_979 at 1107.894 kb on + strand, within Pf1N1B4_979 at 1107.894 kb on + strand, within Pf1N1B4_979 at 1107.894 kb on + strand, within Pf1N1B4_979 at 1107.894 kb on + strand, within Pf1N1B4_979 at 1107.894 kb on + strand, within Pf1N1B4_979 at 1107.894 kb on + strand, within Pf1N1B4_979 at 1107.895 kb on - strand, within Pf1N1B4_979 at 1107.895 kb on - strand, within Pf1N1B4_979 at 1107.895 kb on - strand, within Pf1N1B4_979 at 1107.895 kb on - strand, within Pf1N1B4_979 at 1107.895 kb on - strand at 1107.895 kb on - strand at 1107.895 kb on - strand, within Pf1N1B4_979 at 1107.895 kb on - strand at 1107.895 kb on - strand, within Pf1N1B4_979 at 1107.895 kb on - strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.897 kb on + strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1107.898 kb on - strand, within Pf1N1B4_979 at 1108.008 kb on + strand, within Pf1N1B4_979 at 1108.008 kb on + strand, within Pf1N1B4_979 at 1108.009 kb on - strand, within Pf1N1B4_979 at 1108.009 kb on - strand, within Pf1N1B4_979 at 1108.328 kb on + strand at 1108.328 kb on + strand at 1108.328 kb on + strand at 1108.328 kb on + strand at 1108.329 kb on - strand at 1108.330 kb on + strand at 1108.379 kb on + strand, within Pf1N1B4_980 at 1108.403 kb on + strand, within Pf1N1B4_980 at 1108.403 kb on + strand, within Pf1N1B4_980 at 1108.404 kb on - strand, within Pf1N1B4_980 at 1108.404 kb on - strand, within Pf1N1B4_980 at 1108.404 kb on - strand, within Pf1N1B4_980 at 1108.404 kb on - strand, within Pf1N1B4_980 at 1108.460 kb on + strand at 1108.460 kb on + strand at 1108.460 kb on + strand at 1108.460 kb on + strand at 1108.460 kb on + strand at 1108.461 kb on - strand at 1108.461 kb on - strand at 1108.461 kb on - strand at 1108.471 kb on + strand at 1108.499 kb on - strand at 1108.499 kb on - strand at 1108.611 kb on - strand, within Pf1N1B4_981 at 1108.793 kb on + strand, within Pf1N1B4_981 at 1108.794 kb on - strand, within Pf1N1B4_981 at 1108.794 kb on - strand, within Pf1N1B4_981 at 1108.794 kb on - strand, within Pf1N1B4_981 at 1108.794 kb on - strand, within Pf1N1B4_981 at 1108.874 kb on + strand, within Pf1N1B4_981 at 1108.874 kb on + strand, within Pf1N1B4_981 at 1108.875 kb on - strand, within Pf1N1B4_981 at 1108.875 kb on - strand, within Pf1N1B4_981 at 1108.875 kb on - strand, within Pf1N1B4_981 at 1108.962 kb on - strand, within Pf1N1B4_981 at 1109.054 kb on + strand, within Pf1N1B4_981 at 1109.054 kb on + strand, within Pf1N1B4_981 at 1109.249 kb on + strand at 1109.267 kb on - strand at 1109.309 kb on + strand at 1109.309 kb on + strand at 1109.539 kb on - strand at 1109.721 kb on - strand at 1109.789 kb on - strand at 1109.943 kb on - strand at 1110.015 kb on - strand at 1110.047 kb on - strand at 1110.125 kb on - strand at 1110.145 kb on + strand at 1110.145 kb on + strand at 1110.146 kb on - strand at 1110.160 kb on - strand at 1110.172 kb on + strand at 1110.242 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 1,107,582 + Pf1N1B4_979 0.37 +0.1 1,107,582 + Pf1N1B4_979 0.37 -1.9 1,107,582 + Pf1N1B4_979 0.37 +0.7 1,107,582 + Pf1N1B4_979 0.37 +0.1 1,107,583 - Pf1N1B4_979 0.37 +0.4 1,107,583 - Pf1N1B4_979 0.37 -1.5 1,107,583 - Pf1N1B4_979 0.37 -0.3 1,107,655 + Pf1N1B4_979 0.43 -0.9 1,107,656 - Pf1N1B4_979 0.43 -0.9 1,107,681 + Pf1N1B4_979 0.45 -1.8 1,107,682 - Pf1N1B4_979 0.46 +0.7 1,107,703 + Pf1N1B4_979 0.47 -1.7 1,107,704 - Pf1N1B4_979 0.47 -0.4 1,107,704 - Pf1N1B4_979 0.47 +1.3 1,107,704 - Pf1N1B4_979 0.47 +0.1 1,107,780 + Pf1N1B4_979 0.54 +0.1 1,107,852 + Pf1N1B4_979 0.61 +0.1 1,107,894 + Pf1N1B4_979 0.64 -1.2 1,107,894 + Pf1N1B4_979 0.64 -0.9 1,107,894 + Pf1N1B4_979 0.64 +0.7 1,107,894 + Pf1N1B4_979 0.64 +0.6 1,107,894 + Pf1N1B4_979 0.64 -0.9 1,107,894 + Pf1N1B4_979 0.64 -1.1 1,107,894 + Pf1N1B4_979 0.64 -0.7 1,107,894 + Pf1N1B4_979 0.64 +0.9 1,107,895 - Pf1N1B4_979 0.64 +1.7 1,107,895 - Pf1N1B4_979 0.64 +1.1 1,107,895 - Pf1N1B4_979 0.64 -0.9 1,107,895 - Pf1N1B4_979 0.64 +0.1 1,107,895 - +0.1 1,107,895 - +0.1 1,107,895 - Pf1N1B4_979 0.64 +0.3 1,107,895 - -0.9 1,107,895 - Pf1N1B4_979 0.64 -0.9 1,107,895 - Pf1N1B4_979 0.64 +0.1 1,107,897 + Pf1N1B4_979 0.65 -1.8 1,107,897 + Pf1N1B4_979 0.65 -0.5 1,107,897 + Pf1N1B4_979 0.65 -0.9 1,107,897 + Pf1N1B4_979 0.65 -2.5 1,107,897 + Pf1N1B4_979 0.65 -0.9 1,107,897 + Pf1N1B4_979 0.65 +1.1 1,107,897 + Pf1N1B4_979 0.65 -1.5 1,107,897 + Pf1N1B4_979 0.65 -1.9 1,107,897 + Pf1N1B4_979 0.65 +1.1 1,107,897 + Pf1N1B4_979 0.65 -1.9 1,107,897 + -1.5 1,107,897 + Pf1N1B4_979 0.65 +1.1 1,107,897 + Pf1N1B4_979 0.65 +0.4 1,107,897 + Pf1N1B4_979 0.65 -1.7 1,107,897 + Pf1N1B4_979 0.65 -1.5 1,107,898 - Pf1N1B4_979 0.65 -0.9 1,107,898 - Pf1N1B4_979 0.65 +1.5 1,107,898 - Pf1N1B4_979 0.65 -0.9 1,107,898 - Pf1N1B4_979 0.65 -2.5 1,107,898 - Pf1N1B4_979 0.65 +0.1 1,107,898 - Pf1N1B4_979 0.65 -1.5 1,107,898 - Pf1N1B4_979 0.65 +1.1 1,107,898 - +1.1 1,107,898 - Pf1N1B4_979 0.65 +0.4 1,107,898 - Pf1N1B4_979 0.65 -0.9 1,107,898 - Pf1N1B4_979 0.65 +1.4 1,108,008 + Pf1N1B4_979 0.74 -0.6 1,108,008 + Pf1N1B4_979 0.74 -1.5 1,108,009 - Pf1N1B4_979 0.75 +0.8 1,108,009 - Pf1N1B4_979 0.75 -0.3 1,108,328 + -0.8 1,108,328 + -0.2 1,108,328 + +1.3 1,108,328 + -1.5 1,108,329 - -1.2 1,108,330 + -0.9 1,108,379 + Pf1N1B4_980 0.28 +2.7 1,108,403 + Pf1N1B4_980 0.47 -0.1 1,108,403 + Pf1N1B4_980 0.47 +0.5 1,108,404 - Pf1N1B4_980 0.47 +1.7 1,108,404 - Pf1N1B4_980 0.47 -0.2 1,108,404 - Pf1N1B4_980 0.47 +0.4 1,108,404 - Pf1N1B4_980 0.47 +0.7 1,108,460 + -1.5 1,108,460 + -0.9 1,108,460 + +0.8 1,108,460 + -0.5 1,108,460 + +0.1 1,108,461 - +0.7 1,108,461 - -0.3 1,108,461 - -1.7 1,108,471 + -1.5 1,108,499 - -0.6 1,108,499 - -2.5 1,108,611 - Pf1N1B4_981 0.10 -1.5 1,108,793 + Pf1N1B4_981 0.33 +0.1 1,108,794 - Pf1N1B4_981 0.34 -1.5 1,108,794 - Pf1N1B4_981 0.34 +0.4 1,108,794 - Pf1N1B4_981 0.34 -0.9 1,108,794 - Pf1N1B4_981 0.34 +0.7 1,108,874 + Pf1N1B4_981 0.44 -0.9 1,108,874 + Pf1N1B4_981 0.44 +0.1 1,108,875 - Pf1N1B4_981 0.44 +1.1 1,108,875 - Pf1N1B4_981 0.44 -2.1 1,108,875 - Pf1N1B4_981 0.44 -1.5 1,108,962 - Pf1N1B4_981 0.55 -1.2 1,109,054 + Pf1N1B4_981 0.67 -0.9 1,109,054 + Pf1N1B4_981 0.67 -1.5 1,109,249 + -0.6 1,109,267 - -3.1 1,109,309 + -0.9 1,109,309 + +1.4 1,109,539 - -0.5 1,109,721 - +0.1 1,109,789 - -1.2 1,109,943 - -0.1 1,110,015 - -1.5 1,110,047 - -0.9 1,110,125 - +0.1 1,110,145 + +0.1 1,110,145 + -2.2 1,110,146 - +2.1 1,110,160 - -0.7 1,110,172 + -0.4 1,110,242 + +0.3
Or see this region's nucleotide sequence