Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_813 and Pf1N1B4_814 are separated by 514 nucleotides Pf1N1B4_814 and Pf1N1B4_815 overlap by 4 nucleotides Pf1N1B4_815 and Pf1N1B4_816 are separated by 161 nucleotides
Pf1N1B4_813 - hypothetical protein, at 931,282 to 931,776
_813
Pf1N1B4_814 - Transcriptional regulator, PadR family, at 932,291 to 932,872
_814
Pf1N1B4_815 - iron-chelator utilization protein, at 932,869 to 933,666
_815
Pf1N1B4_816 - Integral membrane protein, at 933,828 to 934,433
_816
Position (kb)
932
933 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 931.337 kb on + strand, within Pf1N1B4_813 at 931.337 kb on + strand, within Pf1N1B4_813 at 931.391 kb on + strand, within Pf1N1B4_813 at 931.434 kb on + strand, within Pf1N1B4_813 at 931.434 kb on + strand, within Pf1N1B4_813 at 931.434 kb on + strand, within Pf1N1B4_813 at 931.435 kb on - strand, within Pf1N1B4_813 at 931.444 kb on + strand, within Pf1N1B4_813 at 931.444 kb on + strand, within Pf1N1B4_813 at 931.479 kb on + strand, within Pf1N1B4_813 at 931.479 kb on + strand, within Pf1N1B4_813 at 931.479 kb on + strand at 931.479 kb on + strand, within Pf1N1B4_813 at 931.480 kb on - strand, within Pf1N1B4_813 at 931.480 kb on - strand, within Pf1N1B4_813 at 931.480 kb on - strand, within Pf1N1B4_813 at 931.480 kb on - strand, within Pf1N1B4_813 at 931.494 kb on - strand, within Pf1N1B4_813 at 931.494 kb on - strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.556 kb on + strand, within Pf1N1B4_813 at 931.557 kb on - strand, within Pf1N1B4_813 at 931.646 kb on + strand, within Pf1N1B4_813 at 931.646 kb on + strand, within Pf1N1B4_813 at 931.646 kb on + strand, within Pf1N1B4_813 at 931.646 kb on + strand, within Pf1N1B4_813 at 931.647 kb on - strand, within Pf1N1B4_813 at 931.778 kb on + strand at 931.779 kb on - strand at 931.781 kb on + strand at 931.797 kb on + strand at 931.797 kb on + strand at 932.108 kb on + strand at 932.146 kb on + strand at 932.148 kb on + strand at 932.148 kb on + strand at 932.148 kb on + strand at 932.251 kb on + strand at 932.267 kb on + strand at 932.460 kb on - strand, within Pf1N1B4_814 at 932.502 kb on - strand, within Pf1N1B4_814 at 932.525 kb on + strand, within Pf1N1B4_814 at 932.626 kb on - strand, within Pf1N1B4_814 at 932.809 kb on + strand, within Pf1N1B4_814 at 932.810 kb on - strand, within Pf1N1B4_814 at 932.989 kb on - strand, within Pf1N1B4_815 at 932.989 kb on - strand, within Pf1N1B4_815 at 932.989 kb on - strand, within Pf1N1B4_815 at 933.044 kb on + strand, within Pf1N1B4_815 at 933.044 kb on + strand, within Pf1N1B4_815 at 933.044 kb on + strand, within Pf1N1B4_815 at 933.045 kb on - strand, within Pf1N1B4_815 at 933.045 kb on - strand, within Pf1N1B4_815 at 933.045 kb on - strand, within Pf1N1B4_815 at 933.045 kb on - strand, within Pf1N1B4_815 at 933.045 kb on - strand, within Pf1N1B4_815 at 933.145 kb on + strand, within Pf1N1B4_815 at 933.146 kb on - strand, within Pf1N1B4_815 at 933.146 kb on - strand, within Pf1N1B4_815 at 933.160 kb on + strand, within Pf1N1B4_815 at 933.310 kb on + strand, within Pf1N1B4_815 at 933.310 kb on + strand, within Pf1N1B4_815 at 933.311 kb on - strand, within Pf1N1B4_815 at 933.311 kb on - strand at 933.311 kb on - strand at 933.311 kb on - strand, within Pf1N1B4_815 at 933.535 kb on + strand, within Pf1N1B4_815 at 933.535 kb on + strand, within Pf1N1B4_815 at 933.536 kb on - strand, within Pf1N1B4_815 at 933.584 kb on + strand, within Pf1N1B4_815 at 933.664 kb on + strand at 933.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 931,337 + Pf1N1B4_813 0.11 -1.0 931,337 + Pf1N1B4_813 0.11 -3.1 931,391 + Pf1N1B4_813 0.22 -1.0 931,434 + Pf1N1B4_813 0.31 -1.5 931,434 + Pf1N1B4_813 0.31 -1.0 931,434 + Pf1N1B4_813 0.31 -2.0 931,435 - Pf1N1B4_813 0.31 -0.7 931,444 + Pf1N1B4_813 0.33 -0.4 931,444 + Pf1N1B4_813 0.33 -1.0 931,479 + Pf1N1B4_813 0.40 -3.1 931,479 + Pf1N1B4_813 0.40 -1.5 931,479 + +0.0 931,479 + Pf1N1B4_813 0.40 -0.8 931,480 - Pf1N1B4_813 0.40 -2.3 931,480 - Pf1N1B4_813 0.40 -0.4 931,480 - Pf1N1B4_813 0.40 +0.7 931,480 - Pf1N1B4_813 0.40 -1.3 931,494 - Pf1N1B4_813 0.43 -1.0 931,494 - Pf1N1B4_813 0.43 -1.5 931,546 + Pf1N1B4_813 0.53 +0.0 931,546 + Pf1N1B4_813 0.53 -2.0 931,546 + Pf1N1B4_813 0.53 -1.0 931,546 + Pf1N1B4_813 0.53 +0.0 931,546 + Pf1N1B4_813 0.53 -1.5 931,546 + Pf1N1B4_813 0.53 -0.5 931,547 - Pf1N1B4_813 0.54 -1.0 931,547 - Pf1N1B4_813 0.54 +0.0 931,547 - Pf1N1B4_813 0.54 +0.0 931,547 - Pf1N1B4_813 0.54 -1.0 931,547 - Pf1N1B4_813 0.54 -0.8 931,547 - Pf1N1B4_813 0.54 -1.0 931,547 - Pf1N1B4_813 0.54 +1.6 931,556 + Pf1N1B4_813 0.55 +0.6 931,557 - Pf1N1B4_813 0.56 -0.2 931,646 + Pf1N1B4_813 0.74 -1.5 931,646 + Pf1N1B4_813 0.74 -0.5 931,646 + Pf1N1B4_813 0.74 -1.5 931,646 + Pf1N1B4_813 0.74 -3.4 931,647 - Pf1N1B4_813 0.74 -0.3 931,778 + -3.1 931,779 - -2.0 931,781 + -1.0 931,797 + -1.5 931,797 + -3.3 932,108 + -1.5 932,146 + +0.0 932,148 + -2.1 932,148 + +0.0 932,148 + -1.5 932,251 + -2.0 932,267 + -4.4 932,460 - Pf1N1B4_814 0.29 +0.0 932,502 - Pf1N1B4_814 0.36 -0.4 932,525 + Pf1N1B4_814 0.40 -2.0 932,626 - Pf1N1B4_814 0.58 +0.0 932,809 + Pf1N1B4_814 0.89 -2.0 932,810 - Pf1N1B4_814 0.89 -1.5 932,989 - Pf1N1B4_815 0.15 +2.4 932,989 - Pf1N1B4_815 0.15 +0.3 932,989 - Pf1N1B4_815 0.15 +0.0 933,044 + Pf1N1B4_815 0.22 -0.4 933,044 + Pf1N1B4_815 0.22 -0.2 933,044 + Pf1N1B4_815 0.22 -1.0 933,045 - Pf1N1B4_815 0.22 -1.5 933,045 - Pf1N1B4_815 0.22 -0.1 933,045 - Pf1N1B4_815 0.22 -1.0 933,045 - Pf1N1B4_815 0.22 -0.5 933,045 - Pf1N1B4_815 0.22 +0.4 933,145 + Pf1N1B4_815 0.35 +0.6 933,146 - Pf1N1B4_815 0.35 -1.0 933,146 - Pf1N1B4_815 0.35 -1.5 933,160 + Pf1N1B4_815 0.36 +0.0 933,310 + Pf1N1B4_815 0.55 -0.5 933,310 + Pf1N1B4_815 0.55 -2.0 933,311 - Pf1N1B4_815 0.55 -0.5 933,311 - +0.0 933,311 - -1.0 933,311 - Pf1N1B4_815 0.55 -0.3 933,535 + Pf1N1B4_815 0.83 -1.0 933,535 + Pf1N1B4_815 0.83 +0.5 933,536 - Pf1N1B4_815 0.84 -0.7 933,584 + Pf1N1B4_815 0.90 +0.5 933,664 + +0.0 933,694 - -1.3
Or see this region's nucleotide sequence