Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_775 and Pf1N1B4_776 overlap by 4 nucleotides Pf1N1B4_776 and Pf1N1B4_777 overlap by 1 nucleotides Pf1N1B4_777 and Pf1N1B4_778 are separated by 118 nucleotides
Pf1N1B4_775 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system (EC 2.7.13.3), at 879,089 to 880,990
_775
Pf1N1B4_776 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 880,987 to 882,315
_776
Pf1N1B4_777 - Membrane protein glpM, at 882,315 to 882,653
_777
Pf1N1B4_778 - hypothetical protein, at 882,772 to 883,662
_778
Position (kb)
880
881
882
883 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 880.623 kb on + strand, within Pf1N1B4_775 at 880.624 kb on - strand, within Pf1N1B4_775 at 880.624 kb on - strand, within Pf1N1B4_775 at 880.624 kb on - strand, within Pf1N1B4_775 at 880.752 kb on - strand, within Pf1N1B4_775 at 880.954 kb on - strand at 880.986 kb on + strand at 881.001 kb on + strand at 881.001 kb on + strand at 881.002 kb on - strand at 881.002 kb on - strand at 881.002 kb on - strand at 881.284 kb on + strand, within Pf1N1B4_776 at 881.382 kb on - strand, within Pf1N1B4_776 at 881.782 kb on - strand, within Pf1N1B4_776 at 881.782 kb on - strand, within Pf1N1B4_776 at 881.896 kb on + strand, within Pf1N1B4_776 at 881.897 kb on - strand, within Pf1N1B4_776 at 881.897 kb on - strand, within Pf1N1B4_776 at 881.897 kb on - strand, within Pf1N1B4_776 at 881.897 kb on - strand, within Pf1N1B4_776 at 881.897 kb on - strand, within Pf1N1B4_776 at 881.915 kb on + strand, within Pf1N1B4_776 at 882.298 kb on + strand at 882.299 kb on - strand at 882.355 kb on + strand, within Pf1N1B4_777 at 882.390 kb on + strand, within Pf1N1B4_777 at 882.391 kb on - strand, within Pf1N1B4_777 at 882.391 kb on - strand, within Pf1N1B4_777 at 882.391 kb on - strand, within Pf1N1B4_777 at 882.393 kb on + strand, within Pf1N1B4_777 at 882.393 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.444 kb on + strand, within Pf1N1B4_777 at 882.445 kb on - strand, within Pf1N1B4_777 at 882.445 kb on - strand, within Pf1N1B4_777 at 882.445 kb on - strand, within Pf1N1B4_777 at 882.445 kb on - strand, within Pf1N1B4_777 at 882.445 kb on - strand, within Pf1N1B4_777 at 882.519 kb on + strand, within Pf1N1B4_777 at 882.519 kb on + strand, within Pf1N1B4_777 at 882.519 kb on + strand, within Pf1N1B4_777 at 882.519 kb on + strand, within Pf1N1B4_777 at 882.519 kb on + strand, within Pf1N1B4_777 at 882.529 kb on - strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.543 kb on + strand, within Pf1N1B4_777 at 882.544 kb on - strand, within Pf1N1B4_777 at 882.544 kb on - strand, within Pf1N1B4_777 at 882.544 kb on - strand, within Pf1N1B4_777 at 882.544 kb on - strand at 882.544 kb on - strand, within Pf1N1B4_777 at 882.544 kb on - strand, within Pf1N1B4_777 at 882.544 kb on - strand, within Pf1N1B4_777 at 882.544 kb on - strand, within Pf1N1B4_777 at 882.544 kb on - strand, within Pf1N1B4_777 at 882.544 kb on - strand, within Pf1N1B4_777 at 882.745 kb on - strand at 882.752 kb on + strand at 882.752 kb on + strand at 882.753 kb on - strand at 882.872 kb on + strand, within Pf1N1B4_778 at 882.872 kb on + strand, within Pf1N1B4_778 at 882.872 kb on + strand, within Pf1N1B4_778 at 882.872 kb on + strand, within Pf1N1B4_778 at 882.873 kb on - strand at 882.873 kb on - strand, within Pf1N1B4_778 at 882.873 kb on - strand, within Pf1N1B4_778 at 882.873 kb on - strand, within Pf1N1B4_778 at 882.873 kb on - strand, within Pf1N1B4_778 at 882.873 kb on - strand, within Pf1N1B4_778 at 882.873 kb on - strand, within Pf1N1B4_778 at 882.876 kb on + strand, within Pf1N1B4_778 at 882.932 kb on + strand, within Pf1N1B4_778 at 882.932 kb on + strand, within Pf1N1B4_778 at 882.932 kb on + strand, within Pf1N1B4_778 at 882.932 kb on + strand, within Pf1N1B4_778 at 882.932 kb on + strand, within Pf1N1B4_778 at 882.933 kb on - strand, within Pf1N1B4_778 at 883.210 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 880,623 + Pf1N1B4_775 0.81 +1.6 880,624 - Pf1N1B4_775 0.81 -2.0 880,624 - Pf1N1B4_775 0.81 -0.2 880,624 - Pf1N1B4_775 0.81 +1.0 880,752 - Pf1N1B4_775 0.87 +0.0 880,954 - +0.5 880,986 + -1.0 881,001 + -0.6 881,001 + -0.2 881,002 - -1.2 881,002 - +0.3 881,002 - -2.3 881,284 + Pf1N1B4_776 0.22 -2.6 881,382 - Pf1N1B4_776 0.30 -1.0 881,782 - Pf1N1B4_776 0.60 -0.8 881,782 - Pf1N1B4_776 0.60 +0.3 881,896 + Pf1N1B4_776 0.68 -1.6 881,897 - Pf1N1B4_776 0.68 -1.3 881,897 - Pf1N1B4_776 0.68 -2.8 881,897 - Pf1N1B4_776 0.68 +0.6 881,897 - Pf1N1B4_776 0.68 -1.0 881,897 - Pf1N1B4_776 0.68 +1.0 881,915 + Pf1N1B4_776 0.70 -0.6 882,298 + -1.6 882,299 - +0.2 882,355 + Pf1N1B4_777 0.12 +0.4 882,390 + Pf1N1B4_777 0.22 +0.0 882,391 - Pf1N1B4_777 0.22 -0.7 882,391 - Pf1N1B4_777 0.22 +0.6 882,391 - Pf1N1B4_777 0.22 -0.6 882,393 + Pf1N1B4_777 0.23 +0.0 882,393 + Pf1N1B4_777 0.23 +2.0 882,444 + Pf1N1B4_777 0.38 -0.7 882,444 + Pf1N1B4_777 0.38 +1.8 882,444 + Pf1N1B4_777 0.38 +0.0 882,444 + Pf1N1B4_777 0.38 -0.5 882,444 + Pf1N1B4_777 0.38 -0.7 882,444 + Pf1N1B4_777 0.38 -2.0 882,444 + Pf1N1B4_777 0.38 +1.0 882,444 + Pf1N1B4_777 0.38 +0.0 882,444 + Pf1N1B4_777 0.38 +1.0 882,444 + Pf1N1B4_777 0.38 -1.2 882,444 + Pf1N1B4_777 0.38 -1.0 882,445 - Pf1N1B4_777 0.38 -1.6 882,445 - Pf1N1B4_777 0.38 -0.6 882,445 - Pf1N1B4_777 0.38 -0.7 882,445 - Pf1N1B4_777 0.38 -1.6 882,445 - Pf1N1B4_777 0.38 -1.6 882,519 + Pf1N1B4_777 0.60 +0.0 882,519 + Pf1N1B4_777 0.60 -0.3 882,519 + Pf1N1B4_777 0.60 -0.8 882,519 + Pf1N1B4_777 0.60 -1.3 882,519 + Pf1N1B4_777 0.60 +0.4 882,529 - Pf1N1B4_777 0.63 +1.3 882,543 + Pf1N1B4_777 0.67 -2.3 882,543 + Pf1N1B4_777 0.67 -1.0 882,543 + Pf1N1B4_777 0.67 -0.7 882,543 + Pf1N1B4_777 0.67 -1.0 882,543 + Pf1N1B4_777 0.67 -1.0 882,543 + Pf1N1B4_777 0.67 -0.4 882,543 + Pf1N1B4_777 0.67 -1.0 882,543 + Pf1N1B4_777 0.67 -0.3 882,543 + Pf1N1B4_777 0.67 -0.2 882,544 - Pf1N1B4_777 0.68 +0.7 882,544 - Pf1N1B4_777 0.68 +0.0 882,544 - Pf1N1B4_777 0.68 +0.0 882,544 - +0.0 882,544 - Pf1N1B4_777 0.68 +0.5 882,544 - Pf1N1B4_777 0.68 -1.0 882,544 - Pf1N1B4_777 0.68 +0.0 882,544 - Pf1N1B4_777 0.68 +1.6 882,544 - Pf1N1B4_777 0.68 +0.8 882,544 - Pf1N1B4_777 0.68 -0.4 882,745 - -1.3 882,752 + -2.1 882,752 + -2.0 882,753 - -1.6 882,872 + Pf1N1B4_778 0.11 -2.0 882,872 + Pf1N1B4_778 0.11 +0.0 882,872 + Pf1N1B4_778 0.11 -1.6 882,872 + Pf1N1B4_778 0.11 +2.0 882,873 - +0.0 882,873 - Pf1N1B4_778 0.11 +1.0 882,873 - Pf1N1B4_778 0.11 -1.6 882,873 - Pf1N1B4_778 0.11 -0.4 882,873 - Pf1N1B4_778 0.11 -1.1 882,873 - Pf1N1B4_778 0.11 -2.0 882,873 - Pf1N1B4_778 0.11 +1.8 882,876 + Pf1N1B4_778 0.12 -1.0 882,932 + Pf1N1B4_778 0.18 +0.4 882,932 + Pf1N1B4_778 0.18 +0.0 882,932 + Pf1N1B4_778 0.18 +1.3 882,932 + Pf1N1B4_778 0.18 +0.0 882,932 + Pf1N1B4_778 0.18 +0.6 882,933 - Pf1N1B4_778 0.18 -2.0 883,210 + -1.0
Or see this region's nucleotide sequence