Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_737 and Pf1N1B4_738 overlap by 8 nucleotides Pf1N1B4_738 and Pf1N1B4_739 are separated by 25 nucleotides Pf1N1B4_739 and Pf1N1B4_740 are separated by 108 nucleotides Pf1N1B4_740 and Pf1N1B4_741 are separated by 86 nucleotides
Pf1N1B4_737 - Electron transport complex protein RnfG, at 840,145 to 840,756
_737
Pf1N1B4_738 - Electron transport complex protein RnfE, at 840,749 to 841,339
_738
Pf1N1B4_739 - Endonuclease III (EC 4.2.99.18), at 841,365 to 842,003
_739
Pf1N1B4_740 - Leucyl-tRNA synthetase, at 842,112 to 842,291
_740
Pf1N1B4_741 - DNA-binding response regulator, LuxR family, at 842,378 to 843,004
_741
Position (kb) 841
842
843 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 840.377 kb on - strand, within Pf1N1B4_737 at 840.377 kb on - strand, within Pf1N1B4_737 at 840.485 kb on + strand, within Pf1N1B4_737 at 840.485 kb on + strand, within Pf1N1B4_737 at 840.485 kb on + strand, within Pf1N1B4_737 at 840.485 kb on + strand, within Pf1N1B4_737 at 840.486 kb on - strand, within Pf1N1B4_737 at 840.555 kb on + strand, within Pf1N1B4_737 at 840.555 kb on + strand, within Pf1N1B4_737 at 840.556 kb on - strand, within Pf1N1B4_737 at 840.556 kb on - strand, within Pf1N1B4_737 at 840.706 kb on + strand at 840.706 kb on + strand at 840.707 kb on - strand at 840.707 kb on - strand at 840.754 kb on + strand at 840.754 kb on + strand at 840.754 kb on + strand at 840.754 kb on + strand at 840.754 kb on + strand at 840.754 kb on + strand at 840.755 kb on - strand at 840.755 kb on - strand at 840.755 kb on - strand at 840.755 kb on - strand at 840.755 kb on - strand at 840.755 kb on - strand at 840.755 kb on - strand at 840.755 kb on - strand at 840.769 kb on - strand at 840.788 kb on - strand at 840.866 kb on + strand, within Pf1N1B4_738 at 840.867 kb on - strand, within Pf1N1B4_738 at 840.867 kb on - strand, within Pf1N1B4_738 at 840.867 kb on - strand, within Pf1N1B4_738 at 840.879 kb on + strand, within Pf1N1B4_738 at 840.879 kb on + strand, within Pf1N1B4_738 at 840.879 kb on + strand at 840.879 kb on + strand at 840.880 kb on - strand, within Pf1N1B4_738 at 840.880 kb on - strand, within Pf1N1B4_738 at 840.933 kb on + strand, within Pf1N1B4_738 at 841.013 kb on + strand, within Pf1N1B4_738 at 841.273 kb on + strand, within Pf1N1B4_738 at 841.274 kb on - strand, within Pf1N1B4_738 at 841.281 kb on + strand at 841.288 kb on - strand at 841.440 kb on + strand, within Pf1N1B4_739 at 841.441 kb on - strand, within Pf1N1B4_739 at 841.441 kb on - strand, within Pf1N1B4_739 at 841.542 kb on - strand, within Pf1N1B4_739 at 841.587 kb on + strand, within Pf1N1B4_739 at 841.587 kb on + strand, within Pf1N1B4_739 at 841.588 kb on - strand, within Pf1N1B4_739 at 841.648 kb on + strand, within Pf1N1B4_739 at 841.649 kb on - strand, within Pf1N1B4_739 at 841.675 kb on + strand, within Pf1N1B4_739 at 841.876 kb on - strand, within Pf1N1B4_739 at 841.876 kb on - strand, within Pf1N1B4_739 at 841.918 kb on - strand, within Pf1N1B4_739 at 841.918 kb on - strand, within Pf1N1B4_739 at 841.977 kb on + strand at 841.978 kb on - strand at 841.978 kb on - strand at 841.978 kb on - strand at 841.978 kb on - strand at 841.978 kb on - strand at 842.069 kb on + strand at 842.071 kb on + strand at 842.071 kb on + strand at 842.072 kb on - strand at 842.094 kb on + strand at 842.095 kb on - strand at 842.105 kb on + strand at 842.106 kb on - strand at 842.111 kb on + strand at 842.111 kb on + strand at 842.112 kb on - strand at 842.112 kb on - strand at 842.140 kb on + strand, within Pf1N1B4_740 at 842.140 kb on + strand, within Pf1N1B4_740 at 842.140 kb on + strand, within Pf1N1B4_740 at 842.140 kb on + strand, within Pf1N1B4_740 at 842.141 kb on - strand, within Pf1N1B4_740 at 842.141 kb on - strand, within Pf1N1B4_740 at 842.287 kb on + strand at 842.287 kb on + strand at 842.287 kb on + strand at 842.287 kb on + strand at 842.288 kb on - strand at 842.296 kb on + strand at 842.296 kb on + strand at 842.296 kb on + strand at 842.296 kb on + strand at 842.297 kb on - strand at 842.324 kb on - strand at 842.327 kb on - strand at 842.327 kb on - strand at 842.327 kb on - strand at 842.382 kb on - strand at 842.459 kb on - strand, within Pf1N1B4_741 at 842.459 kb on - strand, within Pf1N1B4_741 at 842.461 kb on - strand, within Pf1N1B4_741 at 842.461 kb on - strand, within Pf1N1B4_741 at 842.461 kb on - strand, within Pf1N1B4_741 at 842.461 kb on - strand, within Pf1N1B4_741 at 842.638 kb on - strand, within Pf1N1B4_741 at 842.638 kb on - strand, within Pf1N1B4_741 at 842.638 kb on - strand, within Pf1N1B4_741 at 842.643 kb on + strand, within Pf1N1B4_741 at 842.664 kb on - strand, within Pf1N1B4_741 at 842.905 kb on + strand, within Pf1N1B4_741 at 842.950 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 840,377 - Pf1N1B4_737 0.38 +0.6 840,377 - Pf1N1B4_737 0.38 -3.2 840,485 + Pf1N1B4_737 0.56 -0.4 840,485 + Pf1N1B4_737 0.56 +1.0 840,485 + Pf1N1B4_737 0.56 +0.2 840,485 + Pf1N1B4_737 0.56 +1.0 840,486 - Pf1N1B4_737 0.56 +2.0 840,555 + Pf1N1B4_737 0.67 -0.1 840,555 + Pf1N1B4_737 0.67 +2.0 840,556 - Pf1N1B4_737 0.67 -1.6 840,556 - Pf1N1B4_737 0.67 +1.0 840,706 + +0.7 840,706 + -0.6 840,707 - -0.8 840,707 - -1.0 840,754 + -1.6 840,754 + +0.4 840,754 + +0.0 840,754 + -1.0 840,754 + +2.0 840,754 + -0.6 840,755 - +1.4 840,755 - -2.3 840,755 - +0.0 840,755 - +0.0 840,755 - -2.8 840,755 - +1.0 840,755 - +1.6 840,755 - +0.2 840,769 - -2.3 840,788 - +0.7 840,866 + Pf1N1B4_738 0.20 -1.6 840,867 - Pf1N1B4_738 0.20 +0.0 840,867 - Pf1N1B4_738 0.20 -1.3 840,867 - Pf1N1B4_738 0.20 -1.7 840,879 + Pf1N1B4_738 0.22 +0.4 840,879 + Pf1N1B4_738 0.22 +1.0 840,879 + +0.0 840,879 + +1.0 840,880 - Pf1N1B4_738 0.22 -0.7 840,880 - Pf1N1B4_738 0.22 +0.1 840,933 + Pf1N1B4_738 0.31 -0.8 841,013 + Pf1N1B4_738 0.45 +1.0 841,273 + Pf1N1B4_738 0.89 +1.3 841,274 - Pf1N1B4_738 0.89 -0.2 841,281 + -1.6 841,288 - -0.4 841,440 + Pf1N1B4_739 0.12 +1.0 841,441 - Pf1N1B4_739 0.12 -0.6 841,441 - Pf1N1B4_739 0.12 +0.0 841,542 - Pf1N1B4_739 0.28 -1.6 841,587 + Pf1N1B4_739 0.35 -2.3 841,587 + Pf1N1B4_739 0.35 -2.6 841,588 - Pf1N1B4_739 0.35 -1.0 841,648 + Pf1N1B4_739 0.44 -2.0 841,649 - Pf1N1B4_739 0.44 -1.0 841,675 + Pf1N1B4_739 0.49 +0.4 841,876 - Pf1N1B4_739 0.80 +0.0 841,876 - Pf1N1B4_739 0.80 -0.5 841,918 - Pf1N1B4_739 0.87 -1.3 841,918 - Pf1N1B4_739 0.87 -1.0 841,977 + +0.0 841,978 - -0.4 841,978 - -2.6 841,978 - -2.6 841,978 - -2.0 841,978 - -1.0 842,069 + -1.2 842,071 + -1.0 842,071 + +0.0 842,072 - +1.0 842,094 + -1.3 842,095 - -0.6 842,105 + -1.0 842,106 - -1.0 842,111 + +1.0 842,111 + -1.0 842,112 - +0.0 842,112 - -0.7 842,140 + Pf1N1B4_740 0.16 -0.8 842,140 + Pf1N1B4_740 0.16 +0.0 842,140 + Pf1N1B4_740 0.16 -0.6 842,140 + Pf1N1B4_740 0.16 +0.3 842,141 - Pf1N1B4_740 0.16 +0.5 842,141 - Pf1N1B4_740 0.16 -3.2 842,287 + +1.0 842,287 + -1.0 842,287 + +0.1 842,287 + +0.0 842,288 - +0.6 842,296 + +1.0 842,296 + -0.8 842,296 + +1.6 842,296 + +1.8 842,297 - +0.6 842,324 - +0.9 842,327 - +0.0 842,327 - +0.0 842,327 - -0.3 842,382 - -1.0 842,459 - Pf1N1B4_741 0.13 -1.0 842,459 - Pf1N1B4_741 0.13 -1.8 842,461 - Pf1N1B4_741 0.13 -2.8 842,461 - Pf1N1B4_741 0.13 -1.0 842,461 - Pf1N1B4_741 0.13 +1.4 842,461 - Pf1N1B4_741 0.13 -1.0 842,638 - Pf1N1B4_741 0.41 +1.3 842,638 - Pf1N1B4_741 0.41 +0.7 842,638 - Pf1N1B4_741 0.41 +0.5 842,643 + Pf1N1B4_741 0.42 +0.0 842,664 - Pf1N1B4_741 0.46 +0.0 842,905 + Pf1N1B4_741 0.84 +0.4 842,950 + +1.6
Or see this region's nucleotide sequence