Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_731 and Pf1N1B4_732 are separated by 55 nucleotides Pf1N1B4_732 and Pf1N1B4_733 are separated by 108 nucleotides Pf1N1B4_733 and Pf1N1B4_734 are separated by 129 nucleotides Pf1N1B4_734 and Pf1N1B4_735 overlap by 4 nucleotides
Pf1N1B4_731 - Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like, at 833,953 to 835,047
_731
Pf1N1B4_732 - hypothetical protein, at 835,103 to 835,306
_732
Pf1N1B4_733 - Methionyl-tRNA synthetase (EC 6.1.1.10), at 835,415 to 837,466
_733
Pf1N1B4_734 - Electron transport complex protein RnfA, at 837,596 to 838,165
_734
Pf1N1B4_735 - Electron transport complex protein RnfB, at 838,162 to 839,169
_735
Position (kb) 835
836
837
838 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 834.903 kb on - strand, within Pf1N1B4_731 at 834.903 kb on - strand, within Pf1N1B4_731 at 834.903 kb on - strand, within Pf1N1B4_731 at 835.090 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.106 kb on + strand at 835.106 kb on + strand at 835.106 kb on + strand at 835.106 kb on + strand at 835.106 kb on + strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.189 kb on + strand, within Pf1N1B4_732 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.465 kb on - strand at 837.465 kb on - strand at 837.558 kb on + strand at 837.758 kb on + strand, within Pf1N1B4_734 at 837.768 kb on - strand, within Pf1N1B4_734 at 837.777 kb on + strand, within Pf1N1B4_734 at 837.778 kb on - strand, within Pf1N1B4_734 at 837.778 kb on - strand, within Pf1N1B4_734 at 837.780 kb on + strand, within Pf1N1B4_734 at 837.780 kb on + strand, within Pf1N1B4_734 at 837.781 kb on - strand, within Pf1N1B4_734 at 837.984 kb on + strand, within Pf1N1B4_734 at 838.233 kb on + strand at 838.233 kb on + strand at 838.233 kb on + strand at 838.233 kb on + strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.241 kb on - strand at 838.241 kb on - strand at 838.384 kb on + strand, within Pf1N1B4_735 at 838.385 kb on - strand, within Pf1N1B4_735 at 838.385 kb on - strand, within Pf1N1B4_735
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 834,903 - Pf1N1B4_731 0.87 +0.3 834,903 - Pf1N1B4_731 0.87 +0.6 834,903 - Pf1N1B4_731 0.87 -0.8 835,090 + +0.6 835,099 + -0.6 835,099 + -0.6 835,099 + +1.0 835,099 + -0.7 835,099 + -1.0 835,099 + -0.8 835,099 + +0.6 835,099 + -0.6 835,099 + -1.3 835,100 - -2.1 835,100 - -2.3 835,100 - -0.2 835,100 - -1.6 835,100 - +1.0 835,100 - +0.0 835,100 - +0.0 835,100 - -1.0 835,106 + +1.0 835,106 + +0.4 835,106 + -1.6 835,106 + +0.0 835,106 + -0.9 835,107 - -1.3 835,107 - +0.0 835,107 - +1.6 835,107 - -0.3 835,107 - -1.0 835,107 - -0.1 835,107 - +0.0 835,189 + Pf1N1B4_732 0.42 -0.8 837,030 + Pf1N1B4_733 0.79 -0.8 837,030 + Pf1N1B4_733 0.79 -0.7 837,030 + Pf1N1B4_733 0.79 -1.6 837,465 - -1.0 837,465 - -0.2 837,558 + +1.0 837,758 + Pf1N1B4_734 0.28 +0.0 837,768 - Pf1N1B4_734 0.30 -0.6 837,777 + Pf1N1B4_734 0.32 -0.7 837,778 - Pf1N1B4_734 0.32 +0.6 837,778 - Pf1N1B4_734 0.32 -1.0 837,780 + Pf1N1B4_734 0.32 -0.6 837,780 + Pf1N1B4_734 0.32 -1.0 837,781 - Pf1N1B4_734 0.32 -1.2 837,984 + Pf1N1B4_734 0.68 -0.2 838,233 + +2.8 838,233 + -2.0 838,233 + +1.6 838,233 + -0.6 838,234 - -1.0 838,234 - -1.6 838,234 - +1.6 838,234 - +0.0 838,234 - -0.7 838,234 - +0.0 838,234 - +0.6 838,234 - +2.2 838,241 - +0.0 838,241 - +1.3 838,384 + Pf1N1B4_735 0.22 -0.4 838,385 - Pf1N1B4_735 0.22 +3.0 838,385 - Pf1N1B4_735 0.22 -2.0
Or see this region's nucleotide sequence